Recombinant Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)
![Western blot - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)](https://www.abcam.com/ps/products/264/ab264557/Images/ab264557-358931-anti-il2-receptor-alpha-antibody-epr22816-65-western-blot-recombinant-protein-mouse.jpg "Western blot - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22816-65] to IL-2 Receptor alpha
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-IL-2 Receptor alpha antibody [EPR22816-65]
See all IL-2 Receptor alpha primary antibodies -
Description
Rabbit monoclonal [EPR22816-65] to IL-2 Receptor alpha -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: His-tagged mouse IL-2 Receptor alpha recombinant protein, Mouse thymus and WEHI-231 lysates. Flow Cyt: Mouse splenocytes cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22816-65 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 31 kDa).
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| Flow Cyt |
1/500.
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 31 kDa). |
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Flow Cyt
1/500. |
Target
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Function
Receptor for interleukin-2. -
Involvement in disease
Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
Sequence similarities
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3559 Human
- Entrez Gene: 16184 Mouse
- Omim: 147730 Human
- SwissProt: P01589 Human
- SwissProt: P01590 Mouse
- Unigene: 231367 Human
- Unigene: 915 Mouse
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Alternative names
- Interleukin 2 receptor alpha chain antibody
- CD25 antibody
- CD25 antigen antibody
see all
Images
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Western blot - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557) at 1/1000 dilution + His-tagged mouse IL-2 Receptor alpha recombinant protein (aa22-236) 10 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 31 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 5 seconds.
This recombinant protein with LIF-tag (LIF tag MW is ~40KD) expressed from a mammalian - HEK-293 expression system.
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![Flow Cytometry - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)](https://www.abcam.com/ps/products/264/ab264557/Images/ab264557-1-anti-il2-receptor-alpha-antibody-epr22816-65-flowcytometry-splenocytes-mouse.jpg)
Flow Cytometry - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)Flow cytometric analysis of Mouse splenocytes cells labelling IL-2 Receptor alpha with ab264557 at 1/500 (Right) as compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG (Left) or ab264557 (Right). Then stained with anti-CD4 conjugated to Alexa Fluor® 647.
Gated on viable cells.
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![Western blot - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)](https://www.abcam.com/ps/products/264/ab264557/Images/ab264557-358930-anti-il2-receptor-alpha-antibody-epr22816-65-western-blot-mouse.jpg)
Western blot - Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)All lanes : Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557) at 1/1000 dilution
Lane 1 : Mouse thymus lysate
Lane 2 : WEHI-231 (mouse B cell lymphoma B lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 31 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lane 1: 3 minutes; Lane 2: 81 seconds.
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![Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)](https://www.abcam.com/ps/products/264/ab264557/Images/ab264557-4-benefits-of-recombinant-antibodies.png)
Anti-IL-2 Receptor alpha antibody [EPR22816-65] (ab264557)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab264557 has not yet been referenced specifically in any publications.