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RecombinantRabMAb

Recombinant Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)

Submit a review Submit a question References (15)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)
  • Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6452] to IL-2 Receptor alpha
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

Overview

  • Product name

    Anti-IL-2 Receptor alpha antibody [EPR6452]
    See all IL-2 Receptor alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR6452] to IL-2 Receptor alpha

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human IL-2 Receptor alpha aa 1-250.
    Database link: P01589

  • Positive control

    • IHC-P: Hodgkin's lymphoma, human tonsil, spleen, liver, and thymus tissues. ICC/IF: Jurkat cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab128955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
ICC/IF
1/100 - 1/250.
Notes
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
ICC/IF
1/100 - 1/250.
Application notes
Is unsuitable for IP.

Target

  • Function

    Receptor for interleukin-2.

  • Involvement in disease

    Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.

  • Sequence similarities

    Contains 2 Sushi (CCP/SCR) domains.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Interleukin 2 receptor alpha chain antibody
      • CD25 antibody
      • CD25 antigen antibody
      • IDDM10 antibody
      • IL 2 receptor alpha subunit antibody
      • IL-2 receptor subunit alpha antibody
      • IL-2-RA antibody
      • IL-2R subunit alpha antibody
      • IL2 RA antibody
      • IL2 Receptor alpha antibody
      • IL2-RA antibody
      • IL2R antibody
      • IL2R, alpha chain antibody
      • IL2RA antibody
      • IL2RA_HUMAN antibody
      • IMD41 antibody
      • Interleukin 2 receptor alpha antibody
      • Interleukin 2 receptor antibody
      • Interleukin-2 receptor subunit alpha antibody
      • p55 antibody
      • t-cell growth factor receptor antibody
      • TAC antibody
      • TAC antigen antibody
      • TCGFR antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)
      Immunohistochemical staining of paraffin embedded human Hodgkin lymphoma with purified ab128955 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Immunocytochemistry/ Immunofluorescence - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)
      Immunocytochemistry/ Immunofluorescence - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)

      Immunofluorescence staining of Jurkat cells with purified ab128955 at a working dilution of 1/200, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab128955 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)

      Unpurified ab128955, at 1/250 dilution, staining IL2 Receptor alpha in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)
      Immunohistochemical staining of paraffin embedded human skeletal muscle with purified ab128955 at a working dilution of 1/100. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)

      Unpurified ab128955 showing positive staining in Hodgkin's lymphoma tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)

      Unpurified ab128955 showing negative staining in Human liver tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)

      Unpurified ab128955 showing positive staining in Human thymus tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)

      Unpurified ab128955 showing positive staining in Human spleen tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)

      Tissue Microarrays stained for "Anti-IL-2 Receptor alpha antibody [EPR6452]” using "ab128955" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab128955 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    • Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)
      Anti-IL-2 Receptor alpha antibody [EPR6452] (ab128955)

    References (15)

    Publishing research using ab128955? Please let us know so that we can cite the reference in this datasheet.

    ab128955 has been referenced in 15 publications.

    • Kemper K  et al. Mechanistic and pharmacodynamic studies of DuoBody-CD3x5T4 in preclinical tumor models. Life Sci Alliance 5:N/A (2022). PubMed: 36271507
    • Zadka Ł  et al. Interplay of stromal tumor-infiltrating lymphocytes, normal colonic mucosa, cancer-associated fibroblasts, clinicopathological data and the immunoregulatory molecules of patients diagnosed with colorectal cancer. Cancer Immunol Immunother 70:2681-2700 (2021). PubMed: 33625532
    • Liang Q  et al. RANK promotes colorectal cancer migration and invasion by activating the Ca2+-calcineurin/NFATC1-ACP5 axis. Cell Death Dis 12:336 (2021). PubMed: 33795653
    • Gong L  et al. CD4+CD25+ Regulatory T Cells in Intracranial Thrombi Are Inversely Correlated with Hemorrhagic Transformation after Thrombectomy: A Clinical-Immunohistochemical Analysis of Acute Ischemic Stroke. Oxid Med Cell Longev 2021:3143248 (2021). PubMed: 34055193
    • Orlovic Vlaho M  et al. CD25+ FOXP3+ and CD4+ CD25+ cells distribution in decidual departments of women with severe and mild pre-eclampsia: Comparison with healthy pregnancies. Am J Reprod Immunol 84:e13281 (2020). PubMed: 32485016
    View all Publications for this product

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