Recombinant Anti-IL-2 Receptor alpha antibody [EPR6452] - BSA and Azide free (ab215378)

Clone EPR6452 (ab215378) has been successfully conjugated by Abcam. This image was generated using Anti-IL-2 Receptor alpha antibody [EPR6452] (Alexa Fluor® 647). Please refer to ab205859 for protocol details.

ab205859 staining IL2 Receptor in K562 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab205859 at 1/100 dilution (shown in red). Cell membranes were labelled with WGA (Alexa Fluor^® 488, shown in green). Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

This product also gave a positive signal under the same testing conditions in K562 cells fixed with 4% formaldehyde (10 min).

Immunohistochemical analysis of paraffin-embedded Human Hodgkin’s lymphoma tissue labeling Anti-IL-2 Receptor alpha with ab215378 at 1/250 dilution (10 μg/ml), followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on human Hodgkin’s lymphoma. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Immunofluorescence staining of Jurkat cells with purified ab128955 at a working dilution of 1/200, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 100% methanol and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab128955 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128955).

Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Anti-IL-2 Receptor alpha with ab215378 at 1/250 dilution (10 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on stromal cells in human lung carcinoma. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128955).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128955).

Unpurified ab128955, at 1/250 dilution, staining IL2 Receptor alpha in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128955).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unpurified ab128955 showing positive staining in Hodgkin's lymphoma tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128955).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unpurified ab128955 showing negative staining in Human liver tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128955).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unpurified ab128955 showing positive staining in Human thymus tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128955).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unpurified ab128955 showing positive staining in Human spleen tissue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128955).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.