Recombinant Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP176] to IL-2 Receptor alpha
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-IL-2 Receptor alpha antibody [SP176]
See all IL-2 Receptor alpha primary antibodies -
Description
Rabbit monoclonal [SP176] to IL-2 Receptor alpha -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MOLT-4, HDLM-2 and WEHI-231 whole cell lysate. IHC-P: Human endometrium and tonsil tissue. ICC/IF: HDLM-2 cells
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP176 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231441 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/200. Predicted molecular weight: 31 kDa.
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IHC-P | (2) |
1/100.
Boil tissue section in EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 min. Incubate with ab231441 for 10 minutes at room temperature. |
ICC/IF | (1) |
1/50.
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Notes |
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WB
1/200. Predicted molecular weight: 31 kDa. |
IHC-P
1/100. Boil tissue section in EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 min. Incubate with ab231441 for 10 minutes at room temperature. |
ICC/IF
1/50. |
Target
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Function
Receptor for interleukin-2. -
Involvement in disease
Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels. -
Sequence similarities
Contains 2 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3559 Human
- Entrez Gene: 16184 Mouse
- Entrez Gene: 25704 Rat
- Omim: 147730 Human
- SwissProt: P01589 Human
- SwissProt: P01590 Mouse
- SwissProt: P26897 Rat
- Unigene: 231367 Human
see all -
Alternative names
- Interleukin 2 receptor alpha chain antibody
- CD25 antibody
- CD25 antigen antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
Formalin-fixed, paraffin-embedded human endometrium tissue stained for IL2 Receptor alpha using ab231441 at 1/100 dilution in immunohistochemical analysis.
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Anti-IL-2 Receptor alpha antibody [SP176] (ab231441) at 0.433 µg/ml + HDLM-2 (Human Hodgkin lymphoma) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 31 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Blocking/Diluting buffer and concentration: 5% NFDM /TBST -
Immunocytochemistry/ Immunofluorescence analysis of HDLM-2 (Human Hodgkin lymphoma) cells labeling IL-2 Receptor alpha with purified ab231441 at 1/50 (9.5µg/mL). Cells were fixed in 100% methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Anti-IL-2 Receptor alpha antibody [SP176] (ab231441) at 0.433 µg/ml + WEHI-231 (Mouse B cell lymphoma B lymphocyte) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 31 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Blocking/Diluting buffer and concentration: 5% NFDM /TBST -
Anti-IL-2 Receptor alpha antibody [SP176] (ab231441) at 1/200 dilution + MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Developed using the ECL technique.
Predicted band size: 31 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for IL2 Receptor alpha using ab231441 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab231441 has been referenced in 5 publications.
- Zhou Y et al. Immunity and Extracellular Matrix Characteristics of Breast Cancer Subtypes Based on Identification by T Helper Cells Profiling. Front Immunol 13:859581 (2022). PubMed: 35795662
- Tian Q et al. The regulatory roles of T helper cells in distinct extracellular matrix characterization in breast cancer. Front Immunol 13:871742 (2022). PubMed: 36159822
- Lee JH et al. Cysteine-specific 89Zr-labeled anti-CD25 IgG allows immuno-PET imaging of interleukin-2 receptor-α on T cell lymphomas. Front Immunol 13:1017132 (2022). PubMed: 36591250
- Cao Q et al. miR-151-5p alleviates corneal allograft rejection by activating PI3K/AKT signaling pathway and balancing Th17/Treg after corneal transplantation via targeting IL-2Rɑ. Ann Transl Med 9:1410 (2021). PubMed: 34733962
- Tillman H et al. Morphologic and Immunohistochemical Characterization of Spontaneous Lymphoma/Leukemia in NSG Mice. Vet Pathol 57:160-171 (2020). PubMed: 31736441