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RecombinantRabMAb

Recombinant Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)

Reviews (5) Submit a question References (5)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
  • Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
  • Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
  • Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
  • Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP176] to IL-2 Receptor alpha
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-IL-2 Receptor alpha antibody [SP176]
    See all IL-2 Receptor alpha primary antibodies

  • Description

    Rabbit monoclonal [SP176] to IL-2 Receptor alpha

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MOLT-4, HDLM-2 and WEHI-231 whole cell lysate. IHC-P: Human endometrium and tonsil tissue. ICC/IF: HDLM-2 cells

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab231441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/200. Predicted molecular weight: 31 kDa.
IHC-P (2)
1/100.

Boil tissue section in EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 min. Incubate with ab231441 for 10 minutes at room temperature.
ICC/IF (1)
1/50.
Notes
WB
1/200. Predicted molecular weight: 31 kDa.
IHC-P
1/100.

Boil tissue section in EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 min. Incubate with ab231441 for 10 minutes at room temperature.
ICC/IF
1/50.

Target

  • Function

    Receptor for interleukin-2.

  • Involvement in disease

    Genetic variations in IL2RA are associated with susceptibility to diabetes mellitus insulin-dependent type 10 (IDDM10) [MIM:601942]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.

  • Sequence similarities

    Contains 2 Sushi (CCP/SCR) domains.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Interleukin 2 receptor alpha chain antibody
    • CD25 antibody
    • CD25 antigen antibody
    • IDDM10 antibody
    • IL 2 receptor alpha subunit antibody
    • IL-2 receptor subunit alpha antibody
    • IL-2-RA antibody
    • IL-2R subunit alpha antibody
    • IL2 RA antibody
    • IL2 Receptor alpha antibody
    • IL2-RA antibody
    • IL2R antibody
    • IL2R, alpha chain antibody
    • IL2RA antibody
    • IL2RA_HUMAN antibody
    • IMD41 antibody
    • Interleukin 2 receptor alpha antibody
    • Interleukin 2 receptor antibody
    • Interleukin-2 receptor subunit alpha antibody
    • p55 antibody
    • t-cell growth factor receptor antibody
    • TAC antibody
    • TAC antigen antibody
    • TCGFR antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)

    Formalin-fixed, paraffin-embedded human endometrium tissue stained for IL2 Receptor alpha using ab231441 at 1/100 dilution in immunohistochemical analysis.

  • Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
    Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
    Anti-IL-2 Receptor alpha antibody [SP176] (ab231441) at 0.433 µg/ml + HDLM-2 (Human Hodgkin lymphoma) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

    Predicted band size: 31 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking/Diluting buffer and concentration: 5% NFDM /TBST
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
    Immunocytochemistry/ Immunofluorescence - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)

    Immunocytochemistry/ Immunofluorescence analysis of HDLM-2 (Human Hodgkin lymphoma) cells labeling IL-2 Receptor alpha with purified ab231441 at 1/50 (9.5µg/mL). Cells were fixed in 100% methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
    Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
    Anti-IL-2 Receptor alpha antibody [SP176] (ab231441) at 0.433 µg/ml + WEHI-231 (Mouse B cell lymphoma B lymphocyte) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

    Predicted band size: 31 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking/Diluting buffer and concentration: 5% NFDM /TBST
  • Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
    Western blot - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
    Anti-IL-2 Receptor alpha antibody [SP176] (ab231441) at 1/200 dilution + MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate

    Developed using the ECL technique.

    Predicted band size: 31 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for IL2 Receptor alpha using ab231441 at 1/100 dilution in immunohistochemical analysis.

  • Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)
    Anti-IL-2 Receptor alpha antibody [SP176] (ab231441)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (5)

Publishing research using ab231441? Please let us know so that we can cite the reference in this datasheet.

ab231441 has been referenced in 5 publications.

  • Zhou Y  et al. Immunity and Extracellular Matrix Characteristics of Breast Cancer Subtypes Based on Identification by T Helper Cells Profiling. Front Immunol 13:859581 (2022). PubMed: 35795662
  • Tian Q  et al. The regulatory roles of T helper cells in distinct extracellular matrix characterization in breast cancer. Front Immunol 13:871742 (2022). PubMed: 36159822
  • Lee JH  et al. Cysteine-specific 89Zr-labeled anti-CD25 IgG allows immuno-PET imaging of interleukin-2 receptor-α on T cell lymphomas. Front Immunol 13:1017132 (2022). PubMed: 36591250
  • Cao Q  et al. miR-151-5p alleviates corneal allograft rejection by activating PI3K/AKT signaling pathway and balancing Th17/Treg after corneal transplantation via targeting IL-2Rɑ. Ann Transl Med 9:1410 (2021). PubMed: 34733962
  • Tillman H  et al. Morphologic and Immunohistochemical Characterization of Spontaneous Lymphoma/Leukemia in NSG Mice. Vet Pathol 57:160-171 (2020). PubMed: 31736441

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence abreview for Anti-IL-2 Receptor alpha antibody [SP176]

 Below Average
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Intestinal organoids treated with +/- H202)
Permeabilization
Yes - Triton X-100 0.5% in 1X PBS pH 7.4
Specification
Intestinal organoids treated with +/- H202
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Mahi Rahman

Verified customer

Submitted Oct 01 2021

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com