Anti-IL-21 antibody (ab154767)
Key features and details
- Rabbit polyclonal to IL-21
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL-21 antibody
See all IL-21 primary antibodies -
Description
Rabbit polyclonal to IL-21 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Monkey
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Immunogen
Recombinant fragment corresponding to Human IL-21 aa 1-155.
Database link: Q9HBE4 -
Positive control
- HL60 whole cell lysate; Human colon carcinoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154767 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 17 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 17 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. -
Tissue specificity
Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. -
Sequence similarities
Belongs to the IL-15/IL-21 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 59067 Human
- Omim: 605384 Human
- SwissProt: Q9HBE4 Human
- Unigene: 567559 Human
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Alternative names
- CVID11 antibody
- IL 21 antibody
- IL-21 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-21 antibody (ab154767)Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling IL21 with ab154767 at 1/500 dilution.
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Western blot - Anti-IL-21 antibody (ab154767)Anti-IL-21 antibody (ab154767) at 1/1000 dilution + HL60 whole cell lysate at 30 µg
Predicted band size: 17 kDa
12% SDS PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab154767 has been referenced in 1 publication.
- Hu J et al. [Immunoexpression and clinical significance of interleukin-21 and receptor activator of nuclear factor ?B ligand in human periapical granulomas and radicular cysts]. Hua Xi Kou Qiang Yi Xue Za Zhi 33:244-8 (2015). PubMed: 26281250