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  1. Link

    products/primary-antibodies/il-21-antibody-ab5978.pdf

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Immunology Innate Immunity Cytokines Interleukins
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Anti-IL-21 antibody (ab5978)

  • Datasheet
Reviews (2) Submit a question References (9)

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Western blot - Anti-IL-21 antibody (ab5978)
  • Western blot - Anti-IL-21 antibody (ab5978)
  • Western blot - Anti-IL-21 antibody (ab5978)
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-21 antibody (ab5978)

Key features and details

  • Rabbit polyclonal to IL-21
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-IL-21 antibody
    See all IL-21 primary antibodies
  • Description

    Rabbit polyclonal to IL-21
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human IL-21 aa 121-135.
    Sequence:

    TCPSCDSYEKKPPKE


    Database link: Q9HBE4
    (Peptide available as ab6025, ab6680)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Purchase matching WB positive control:Recombinant human IL-21 protein
  • General notes

    A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    A novel cytokine was recently identified in human and mouse and designated IL-21, which has significant homology to IL-2, IL-4, and IL-15. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. IL-21 and its receptor activate JAK-STAT signaling pathway. IL-21 is expressed in activated T cells, and HL-60 and THP-1 cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interleukins
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant Human IL-21 protein (ab60287)
    • THP-1 whole cell lysate (ab7913)
    • HL-60 whole cell lysate (ab7914)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab5978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 2 µg/ml.
WB (1)
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 18 kDa.
Notes
ICC/IF
Use a concentration of 2 µg/ml.
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 18 kDa.

Target

  • Function

    Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation.
  • Tissue specificity

    Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes.
  • Sequence similarities

    Belongs to the IL-15/IL-21 family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q9HBE4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 59067 Human
    • Omim: 605384 Human
    • SwissProt: Q9HBE4 Human
    • Unigene: 567559 Human
    • Alternative names

      • CVID11 antibody
      • IL 21 antibody
      • IL-21 antibody
      • Il21 antibody
      • IL21_HUMAN antibody
      • Interleukin 21 antibody
      • Interleukin-21 antibody
      • interleukin-21 isoform antibody
      • Interleukin21 antibody
      • OTTHUMP00000164088 antibody
      • Za11 antibody
      see all

    Images

    • Western blot - Anti-IL-21 antibody (ab5978)
      Western blot - Anti-IL-21 antibody (ab5978)

      All lanes :  Anti-IL-21 antibody (ab5978) at 1 µg/mL, 1h incubation at RT in 5% NFM/TBST.

      Loading: 15 μg of lysates per lane.

      Secondary: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution.

    • Western blot - Anti-IL-21 antibody (ab5978)
      Western blot - Anti-IL-21 antibody (ab5978)
      Western blot analysis of IL-21 expression in HL-60 cell lysate in the absence (lane A) or presence of blocking peptide (lane B) with ab5978 at 1 µg /ml. Western blot analysis of IL-21 expression in HL-60 cell lysate in the absence (lane A) or presence of blocking peptide (lane B) with ab5978 at 1 µg /ml.
    • Western blot - Anti-IL-21 antibody (ab5978)
      Western blot - Anti-IL-21 antibody (ab5978)
      Anti-IL-21 antibody (ab5978) at 1 µg/ml + Recombinant human IL-21 protein (ab73250) at 0.1 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Exposure time: 8 minutes
    • Immunocytochemistry/ Immunofluorescence - Anti-IL-21 antibody (ab5978)
      Immunocytochemistry/ Immunofluorescence - Anti-IL-21 antibody (ab5978)
      ab5978 at 2ug/ml staining of IL21 in K562 cells.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (9)

    Publishing research using ab5978? Please let us know so that we can cite the reference in this datasheet.

    ab5978 has been referenced in 9 publications.

    • Wang N  et al. A novel vaccinia virus enhances anti-tumor efficacy and promotes a long-term anti-tumor response in a murine model of colorectal cancer. Mol Ther Oncolytics 20:71-81 (2021). PubMed: 33575472
    • Liu Z  et al. Circulating follicular T helper cells and humoral reactivity in rheumatic heart disease. Life Sci 245:117390 (2020). PubMed: 32007574
    • Liu Z  et al. IL-21 promotes osteoblastic differentiation of human valvular interstitial cells through the JAK3/STAT3 pathway. Int J Med Sci 17:3065-3072 (2020). PubMed: 33173427
    • Sadeghpour S  et al. Effects of treatment with hydroxychloroquine on the modulation of Th17/Treg ratio and pregnancy outcomes in women with recurrent implantation failure: clinical trial. Immunopharmacol Immunotoxicol 42:632-642 (2020). PubMed: 33081532
    • Xue D  et al. IL-21/IL-21R inhibit tumor growth and invasion in non-small cell lung cancer cells via suppressing Wnt/ß-catenin signaling and PD-L1 expression. Int J Mol Med 44:1697-1706 (2019). PubMed: 31573051
    • Tan L  et al. Chitosan nanoparticle-based delivery of fused NKG2D-IL-21 gene suppresses colon cancer growth in mice. Int J Nanomedicine 12:3095-3107 (2017). WB . PubMed: 28450784
    • Liu T  et al. Upregulation of Interleukin 21 and Interleukin 21 Receptor in Patients with Dermatomyositis and Polymyositis. Chin Med J (Engl) 130:2101-2106 (2017). PubMed: 28836555
    • Qiu F  et al. A genome-wide association study identifies six novel risk loci for primary biliary cholangitis. Nat Commun 8:14828 (2017). IHC-P ; Human . PubMed: 28425483
    • Lee HK  et al. Natural allelic variation of the IL-21 receptor modulates ischemic stroke infarct volume. J Clin Invest 126:2827-38 (2016). PubMed: 27400126

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-21 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MS. Hongyan Zhao

    Verified customer

    Submitted Feb 25 2014

    Western blot abreview for Anti-IL-21 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    60 µg
    Treatment
    transfection of plasmid expressing IL21
    Specification
    293T
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 10 2012

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