Recombinant Anti-IL-21 antibody [EPR22618-28] (BSA and Azide free) (ab282737)
![Flow Cytometry (Intracellular) - Anti-IL-21 antibody [EPR22618-28] (BSA and Azide free) (ab282737)](https://www.abcam.com/ps/products/282/ab282737/Images/ab282737-488272-anti-IL21antibody-EPR2261828-flow-cytometry-th17-mouse.jpg "Flow Cytometry (Intracellular) - Anti-IL-21 antibody [EPR22618-28] (BSA and Azide free) (ab282737)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22618-28] to IL-21 - BSA and Azide free
- Suitable for: Flow Cyt (Intra)
- Reacts with: Mouse
Overview
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Product name
Anti-IL-21 antibody [EPR22618-28] (BSA and Azide free)
See all IL-21 primary antibodies -
Description
Rabbit monoclonal [EPR22618-28] to IL-21 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra)more details
Unsuitable for: IHC-P or WB -
Species reactivity
Reacts with: Mouse
Does not react with: Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt (Intra): Th17 polarized Mouse splenocytes treated with ionomycin then Brefeldin A; 293T (Human embryonic kidney epithelial cell) transfected with HA tagged IL-21 construct.
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General notes
ab282737 is the carrier-free version of ab227837.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22618-28 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab282737 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/500. |
Target
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Function
Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation. -
Tissue specificity
Expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. -
Sequence similarities
Belongs to the IL-15/IL-21 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 60505 Mouse
- SwissProt: Q9ES17 Mouse
- Unigene: 157689 Mouse
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Alternative names
- CVID11 antibody
- IL 21 antibody
- IL-21 antibody
see all
Images
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Flow Cytometry (Intracellular) - Anti-IL-21 antibody [EPR22618-28] (BSA and Azide free) (ab282737)
This data was developed using ab227837, the same antibody clone in a different buffer formulation.
Flow Cytometry (Intracellular) analysis of Th17 polarized mouse splenocytes treated with 500 ng/ml PMA and 500 ng/ml ionomycin for 1 hour, followed by addition of 1 μg/ml Brefeldin A for another 4 hours. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol, and labeling IL-21 with ab282737 at a 1/500 dilution (Right). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at a 1/2000 dilution. Rabbit monoclonal IgG (ab172730) was used as an Isotype control (Left).
IL21 is mutual exclusively expressed with IFN gamma (Th1 marker).
Cells were provided by an anonymous collaborator.
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![Flow Cytometry (Intracellular) - Anti-IL-21 antibody [EPR22618-28] (BSA and Azide free) (ab282737)](https://www.abcam.com/ps/products/282/ab282737/Images/ab282737-488271-anti-IL21antibody-EPR2261828-flow-cytometry-human-293t.jpg)
Flow Cytometry (Intracellular) - Anti-IL-21 antibody [EPR22618-28] (BSA and Azide free) (ab282737)This data was developed using ab227837, the same antibody clone in a different buffer formulation.
Flow Cytometry (Intracellular) analysis of 293T (human embryonic kidney epithelial cell) transfected with HA-tagged IL-21 construct labeling IL-21 with ab282737 at a 1/500 dilution (Right). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at a 1/2000 dilution. Rabbit monoclonal IgG (ab172730) was used as an Isotype control (Left).
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![Anti-IL-21 antibody [EPR22618-28] (BSA and Azide free) (ab282737)](https://www.abcam.com/ps/products/282/ab282737/Images/ab282737-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-IL-21 antibody [EPR22618-28] (BSA and Azide free) (ab282737)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab282737 has not yet been referenced specifically in any publications.
