Anti-IL-21R antibody (ab5980)
Key features and details
- Rabbit polyclonal to IL-21R
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL-21R antibody
See all IL-21R primary antibodies -
Description
Rabbit polyclonal to IL-21R -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human IL-21R aa 97-111.
Sequence:NITDQSGNYSQECGS
(Peptide available asab6225) -
Positive control
- WB: HepG2 cell lysate. IHC-P: Rat lung tissue. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
A novel cytokine related to IL-2 and IL-15 was recently identified and designated IL-21. The receptor for IL-21 (IL-21R, also termed NILR for novel Interleukin receptor) is a new member of the class I cytokine receptor family. IL-21R forms a complex with the common cytokine receptor g chain, gc, and mediates IL-21 signaling. Both IL-21R and the gc are necessary for IL-21 function. IL-21 and its receptor activate the JAK-STAT signaling pathway. IL-21R is expressed in spleen, thymus, natural killer (NK), T and B cell lines. IL-21 plays a role in the proliferation and maturation of NK, B and T cell populations. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5980 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 20 µg/ml.
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| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 60 kDa.
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| Notes |
|---|
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ICC/IF
Use a concentration of 20 µg/ml. |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 60 kDa. |
Target
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Function
This is a receptor for interleukin-21. -
Tissue specificity
Selectively expressed in lymphoid tissues. Most highly expressed in thymus and spleen. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 2 fibronectin type-III domains. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Post-translational
modificationsC-mannosylated at Trp-214 in the WSXWS motif, the sugar chain makes extensive hydrogen bonds with Asn-73 sugar, and bridges the two fibronectin domains transforming the V-shaped receptor into an A-frame. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 50615 Human
- Omim: 605383 Human
- SwissProt: Q9HBE5 Human
- Unigene: 210546 Human
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Alternative names
- CD360 antibody
- IL 21R antibody
- IL-21 receptor antibody
see all
Images
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Western blot - Anti-IL-21R antibody (ab5980)Lane 1 : Anti-IL-21R antibody (ab5980) at 1 µg/ml
Lane 2 : Anti-IL-21R antibody (ab5980) at 2 µg/ml
All lanes : HepG2 cell lysate -
Immunocytochemistry/ Immunofluorescence - Anti-IL-21R antibody (ab5980)Immunocytochemistry/Immunofluorescence labelling A431 cells with ab5980 at 20µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab5980 has been referenced in 8 publications.
- Long ZY et al. Expression and Correlation of IgG4 and IL-21 in Collagen-Induced Arthritis Rats. J Inflamm Res 14:5051-5058 (2021). PubMed: 34629885
- Liu Z et al. IL-21 promotes osteoblastic differentiation of human valvular interstitial cells through the JAK3/STAT3 pathway. Int J Med Sci 17:3065-3072 (2020). PubMed: 33173427
- Guo L et al. Elevated serum IL-21 levels are associated with stable immune status in kidney transplant recipients and a mouse model of kidney transplantation. Aging (Albany NY) 12:18396-18414 (2020). PubMed: 32991326
- Yan L et al. IL-21R functions as an oncogenic factor and is regulated by the lncRNA MALAT1/miR-125a-3p axis in gastric cancer. Int J Oncol 54:7-16 (2019). PubMed: 30387833
- Xue D et al. IL-21/IL-21R inhibit tumor growth and invasion in non-small cell lung cancer cells via suppressing Wnt/ß-catenin signaling and PD-L1 expression. Int J Mol Med 44:1697-1706 (2019). PubMed: 31573051
- Zhang LL et al. Genome profiling revealed the activation of IL2RG/JAK3/STAT5 in peripheral T-cell lymphoma expressing the ITK-SYK fusion gene. Int J Oncol 55:1077-1089 (2019). PubMed: 31545408
- Xing R et al. Interleukin-21 promotes osteoclastogenesis in RAW264.7 cells through the PI3K/AKT signaling pathway independently of RANKL. Int J Mol Med 38:1125-34 (2016). PubMed: 27599586
- Wang T et al. Endothelial interleukin-21 receptor up-regulation in peripheral artery disease. Vasc Med 21:99-104 (2016). PubMed: 26705256