Anti-IL-22RA1 antibody (ab5984)
Key features and details
- Rabbit polyclonal to IL-22RA1
- Suitable for: WB, IHC-P, ICC, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-IL-22RA1 antibody
See all IL-22RA1 primary antibodies -
Description
Rabbit polyclonal to IL-22RA1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
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Positive control
- WB: HepG2 whole cell lysate (ab7900). IHC-P: Rat kidney tissue. ICC/IF: HepG2 cells. ICC: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
IL-22RA1 Antibody is affinity chromatography purified via peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5984 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 62 kDa.
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| IHC-P | (1) |
Use a concentration of 5 µg/ml.
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| ICC |
Use a concentration of 10 µg/ml.
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| ICC/IF |
Use a concentration of 10 mg/ml.
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| Notes |
|---|
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 62 kDa. |
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IHC-P
Use a concentration of 5 µg/ml. |
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ICC
Use a concentration of 10 µg/ml. |
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ICC/IF
Use a concentration of 10 mg/ml. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 58985 Human
- Entrez Gene: 362629 Rat
- Omim: 605457 Human
- SwissProt: Q8N6P7 Human
- Unigene: 110915 Human
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Alternative names
- CRF2 9 antibody
- CRF2-9 antibody
- Cytokine receptor class-II member 9 antibody
see all
Images
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Western blot - Anti-IL-22RA1 antibody (ab5984)Lane 1 : Anti-IL-22RA1 antibody (ab5984) at 0.5 µg/ml
Lane 2 : Anti-IL-22RA1 antibody (ab5984) at 1 µg/ml
All lanes : HepG2 cell lysates -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-22RA1 antibody (ab5984)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling IL-22RA1 with ab5984 at 5µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-22RA1 antibody (ab5984)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling IL-22RA1 with ab5984 at 20µg/ml.
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Immunocytochemistry/ Immunofluorescence - Anti-IL-22RA1 antibody (ab5984)Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling IL-22RA1 with ab5984 at 10µg/ml.
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Immunocytochemistry - Anti-IL-22RA1 antibody (ab5984)Immunocytochemistry of IL-22R in HepG2 cells with IL-22RA1 antibody at 10 µg/ml.
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Western blot - Anti-IL-22RA1 antibody (ab5984)Western blot analysis of IL-22RA1 expression in human HepG2 cell lysate with ab5984 at 1µg /ml. Western blot analysis of IL-22R expression in human HepG2 cell lysate with ab5984 at 1µg /ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab5984 has been referenced in 19 publications.
- Zhang X et al. Folate Receptor 4-Expressing T cell Is Associated with Disease-Free Survival in Patients with Esophageal Squamous Cell Carcinoma. Dis Markers 2022:4351949 (2022). PubMed: 35756495
- Zhao J et al. Tumor Cells Interleukin-22 Expression Associates with Elevated Tumor-Associated Macrophages Infiltrating and Poor Prognosis in Patients with Breast Cancer. Cancer Biother Radiopharm 36:160-166 (2021). PubMed: 33090014
- Wang HH et al. Interleukin-24 protects against liver injury in mouse models. EBioMedicine 64:103213 (2021). PubMed: 33508745
- Mo Y et al. Microbiome-Metabolomics Analysis Reveals the Protection Mechanism of α-Ketoacid on Adenine-Induced Chronic Kidney Disease in Rats. Front Pharmacol 12:657827 (2021). PubMed: 34045965
- Liu J et al. IL-22/IL-22R1 promotes proliferation and collagen synthesis of MRC-5 cells via the JAK/STAT3 signaling pathway and regulates airway subepithelial fibrosis. Exp Ther Med 20:2148-2156 (2020). PubMed: 32765690