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  1. Link

    products/primary-antibodies/il-22ra1-antibody-ab5984.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-IL-22RA1 antibody (ab5984)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (19)

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Western blot - Anti-IL-22RA1 antibody (ab5984)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-22RA1 antibody (ab5984)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-22RA1 antibody (ab5984)
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-22RA1 antibody (ab5984)
  • Immunocytochemistry - Anti-IL-22RA1 antibody (ab5984)
  • Western blot - Anti-IL-22RA1 antibody (ab5984)

Key features and details

  • Rabbit polyclonal to IL-22RA1
  • Suitable for: WB, IHC-P, ICC, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-IL-22RA1 antibody
    See all IL-22RA1 primary antibodies
  • Description

    Rabbit polyclonal to IL-22RA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human IL-22RA1 aa 31-46 (N terminal).
    Sequence:

    QSSNFENILTWDSGPE


    Database link: Q8N6P7
    (Peptide available as ab6229)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: HepG2 whole cell lysate (ab7900). IHC-P: Rat kidney tissue. ICC/IF: HepG2 cells. ICC: HepG2 cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    IL-22RA1 Antibody is affinity chromatography purified via peptide column.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Signal Transduction
    • Adapters
    • Transmembrane

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab5984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 62 kDa.
IHC-P (1)
Use a concentration of 5 µg/ml.
ICC
Use a concentration of 10 µg/ml.
ICC/IF
Use a concentration of 10 mg/ml.
Notes
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 62 kDa.
IHC-P
Use a concentration of 5 µg/ml.
ICC
Use a concentration of 10 µg/ml.
ICC/IF
Use a concentration of 10 mg/ml.

Target

  • Target information above from: UniProt accession Q8N6P7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 58985 Human
    • Entrez Gene: 362629 Rat
    • Omim: 605457 Human
    • SwissProt: Q8N6P7 Human
    • Unigene: 110915 Human
    • Alternative names

      • CRF2 9 antibody
      • CRF2-9 antibody
      • Cytokine receptor class-II member 9 antibody
      • cytokine receptor classII member 9 antibody
      • Cytokine receptor family 2 member 9 antibody
      • I22R1_HUMAN antibody
      • IL-22 receptor subunit alpha-1 antibody
      • IL-22R-alpha-1 antibody
      • IL-22RA1 antibody
      • IL22 Receptor Alpha antibody
      • IL22R antibody
      • IL22R1 antibody
      • Il22ra1 antibody
      • ILTIF R1 chain antibody
      • Interleukin 22 Receptor alpha 1 antibody
      • Interleukin 22 Receptor antibody
      • Interleukin-22 receptor subunit alpha-1 antibody
      • ZcytoR11 antibody
      see all

    Images

    • Western blot - Anti-IL-22RA1 antibody (ab5984)
      Western blot - Anti-IL-22RA1 antibody (ab5984)
      Lane 1 : Anti-IL-22RA1 antibody (ab5984) at 0.5 µg/ml
      Lane 2 : Anti-IL-22RA1 antibody (ab5984) at 1 µg/ml

      All lanes : HepG2 cell lysates
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-22RA1 antibody (ab5984)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-22RA1 antibody (ab5984)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling IL-22RA1 with ab5984 at 5µg/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-22RA1 antibody (ab5984)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-22RA1 antibody (ab5984)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling IL-22RA1 with ab5984 at 20µg/ml.

    • Immunocytochemistry/ Immunofluorescence - Anti-IL-22RA1 antibody (ab5984)
      Immunocytochemistry/ Immunofluorescence - Anti-IL-22RA1 antibody (ab5984)

      Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling IL-22RA1 with ab5984 at 10µg/ml.

    • Immunocytochemistry - Anti-IL-22RA1 antibody (ab5984)
      Immunocytochemistry - Anti-IL-22RA1 antibody (ab5984)

      Immunocytochemistry of IL-22R in HepG2 cells with IL-22RA1 antibody at 10 µg/ml.

    • Western blot - Anti-IL-22RA1 antibody (ab5984)
      Western blot - Anti-IL-22RA1 antibody (ab5984)

      Western blot analysis of IL-22RA1 expression in human HepG2 cell lysate with ab5984 at 1µg /ml. Western blot analysis of IL-22R expression in human HepG2 cell lysate with ab5984 at 1µg /ml.

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (19)

    Publishing research using ab5984? Please let us know so that we can cite the reference in this datasheet.

    ab5984 has been referenced in 19 publications.

    • Zhang X  et al. Folate Receptor 4-Expressing T cell Is Associated with Disease-Free Survival in Patients with Esophageal Squamous Cell Carcinoma. Dis Markers 2022:4351949 (2022). PubMed: 35756495
    • Zhao J  et al. Tumor Cells Interleukin-22 Expression Associates with Elevated Tumor-Associated Macrophages Infiltrating and Poor Prognosis in Patients with Breast Cancer. Cancer Biother Radiopharm 36:160-166 (2021). PubMed: 33090014
    • Wang HH  et al. Interleukin-24 protects against liver injury in mouse models. EBioMedicine 64:103213 (2021). PubMed: 33508745
    • Mo Y  et al. Microbiome-Metabolomics Analysis Reveals the Protection Mechanism of α-Ketoacid on Adenine-Induced Chronic Kidney Disease in Rats. Front Pharmacol 12:657827 (2021). PubMed: 34045965
    • Liu J  et al. IL-22/IL-22R1 promotes proliferation and collagen synthesis of MRC-5 cells via the JAK/STAT3 signaling pathway and regulates airway subepithelial fibrosis. Exp Ther Med 20:2148-2156 (2020). PubMed: 32765690
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IL-22RA1 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Small intestinal tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer ph 6
    Permeabilization
    No
    Specification
    Small intestinal tissue
    Blocking step
    CAS block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 27 2019

    Western blot abreview for Anti-IL22 Receptor Alpha antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Lung)
    Gel Running Conditions
    Reduced Denaturing (12% Bis-Tris NuPAGE)
    Loading amount
    40 µg
    Specification
    Lung
    Blocking step
    Odyssey TBS Blocking Buffer (proprietary) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Pete Serbedzija

    Verified customer

    Submitted Jul 05 2016

    Western blot abreview for Anti-IL22 Receptor Alpha antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    100 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Tissue lysate - whole (Lung)
    Specification
    Lung
    Blocking step
    Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 25 2014

    Flow Cytometry abreview for Anti-IL22 Receptor Alpha antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Fixation
    Surface staining
    Permeabilization
    No
    Sample
    Human Cell (Epidermal keratinocytes)
    Specification
    Epidermal keratinocytes
    Gating Strategy
    Human epidermal keratinocytes
    Preparation
    Cell harvesting/tissue preparation method: Trypsinization
    Sample buffer: Biolegend cell staining buffer
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 27 2014

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