Recombinant Anti-IL-2RG antibody [EPR24561-27] (ab281845)
![Immunocytochemistry/ Immunofluorescence - Anti-IL-2RG antibody [EPR24561-27] (ab281845)](https://www.abcam.com/ps/products/281/ab281845/Images/ab281845-1-anti-il2rg-antibody-epr2456127-immunocytochemistry-ctll2.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IL-2RG antibody [EPR24561-27] (ab281845)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24561-27] to IL-2RG
- Suitable for: ICC/IF, Flow Cyt, IP
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-IL-2RG antibody [EPR24561-27]
See all IL-2RG primary antibodies -
Description
Rabbit monoclonal [EPR24561-27] to IL-2RG -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt, IPmore details
Unsuitable for: IHC-P or WB -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: CTLL-2, U-937 cells. Flow Cyt: Human PBMCs, U937, HT-29, Mouse blood cells. IP: U-937 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24561-27 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab281845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100.
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| Flow Cyt |
1/50.
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| IP |
1/30.
|
| Notes |
|---|
|
ICC/IF
1/100. |
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Flow Cyt
1/50. |
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IP
1/30. |
Target
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Function
Common subunit for the receptors for a variety of interleukins. -
Involvement in disease
Defects in IL2RG are the cause of severe combined immunodeficiency X-linked T-cell-negative/B-cell-positive/NK-cell-negative (XSCID) [MIM:300400]; also known as agammaglobulinemia Swiss type. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Defects in IL2RG are the cause of X-linked combined immunodeficiency (XCID) [MIM:312863]. XCID is a less severe form of X-linked immunodeficiency with a less severe degree of deficiency in cellular and humoral immunity than that seen in XSCID. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 5 subfamily.
Contains 1 fibronectin type-III domain. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3561 Human
- Entrez Gene: 16186 Mouse
- Omim: 308380 Human
- SwissProt: P31785 Human
- SwissProt: P34902 Mouse
- Unigene: 84 Human
- Unigene: 2923 Mouse
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Alternative names
- CD132 antibody
- CD132 antigen antibody
- CIDX antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-IL-2RG antibody [EPR24561-27] (ab281845)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized CTLL-2 cells labelling IL-2RG with ab281845 at 1/100 (5.59 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in CTLL-2 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-IL-2RG antibody [EPR24561-27] (ab281845)](https://www.abcam.com/ps/products/281/ab281845/Images/ab281845-2-anti-il2rg-antibody-epr2456127-immunocytochemistry-u937.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IL-2RG antibody [EPR24561-27] (ab281845)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-937 cells labelling IL-2RG with ab281845 at 1/100 (5.59 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in U-937 cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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![Flow Cytometry - Anti-IL-2RG antibody [EPR24561-27] (ab281845)](https://www.abcam.com/ps/products/281/ab281845/Images/ab281845-3-anti-il2rg-antibody-epr2456127-flow-cytometry-human-peripheral-blood-mononuclear-cell.jpg)
Flow Cytometry - Anti-IL-2RG antibody [EPR24561-27] (ab281845)Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling IL-2RG with ab281845 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab281845. Then stained with anti-CD4 conjugated to Alexa Fluor® 647. Gated on viable cells.
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![Flow Cytometry - Anti-IL-2RG antibody [EPR24561-27] (ab281845)](https://www.abcam.com/ps/products/281/ab281845/Images/ab281845-4-anti-il2rg-antibody-epr2456127-flow-cytometry-ht29.jpg)
Flow Cytometry - Anti-IL-2RG antibody [EPR24561-27] (ab281845)Flow cytometric analysis of HT-29 (Human colorectal adenocarcinoma epithelial cell, Left)/ U937 (human histiocytic lymphoma monocyte, Right) cells labelling IL-2RG with ab281845 at 1/50 dilution (1ug)/ (red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: HT-29 (PMID: 8530527) Gated on viable cells.
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![Flow Cytometry - Anti-IL-2RG antibody [EPR24561-27] (ab281845)](https://www.abcam.com/ps/products/281/ab281845/Images/ab281845-5-anti-il2rg-antibody-epr2456127-flow-cytometry-mouse-blood-cells.jpg)
Flow Cytometry - Anti-IL-2RG antibody [EPR24561-27] (ab281845)Flow cytometric analysis of Mouse blood cells cells labelling IL-2RG with ab281845 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab281845. Then stained with anti-CD4 conjugated to Alexa Fluor® 647. Gated on viable cells.
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![Immunoprecipitation - Anti-IL-2RG antibody [EPR24561-27] (ab281845)](https://www.abcam.com/ps/products/281/ab281845/Images/ab281845-6-anti-il2rg-antibody-epr2456127-immunoprecipitation-u937.jpg)
Immunoprecipitation - Anti-IL-2RG antibody [EPR24561-27] (ab281845)IL-2RG was immunoprecipitated from 0.35 mg U-937 (human histiocytic lymphoma monocyte) whole cell lysate 10 ug with ab281845 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab281845 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: U-937 (human histiocytic lymphoma monocyte) whole cell lysate 10 ug
Lane 2: ab281845 IP in U-937 whole cell lysate 10 ug
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab281845 in U-937 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
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![Anti-IL-2RG antibody [EPR24561-27] (ab281845)](https://www.abcam.com/ps/products/281/ab281845/Images/ab281845-7-Benefits-of-Recombinant-antibodies.jpg)
Anti-IL-2RG antibody [EPR24561-27] (ab281845)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab281845 has not yet been referenced specifically in any publications.