Recombinant Anti-IL-33 antibody [EPR17831] (ab187060)
![Western blot - Anti-IL-33 antibody [EPR17831] (ab187060)](https://www.abcam.com/ps/products/187/ab187060/Images/ab187060-296100-anti-il-33-antibody-epr17831-western-blot.jpg "Western blot - Anti-IL-33 antibody [EPR17831] (ab187060)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17831] to IL-33
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-IL-33 antibody [EPR17831]
See all IL-33 primary antibodies -
Description
Rabbit monoclonal [EPR17831] to IL-33 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat and mouse lung tissue lysate; RAW 264.7 treated with 50 nM Phorbol-12-myristate-13-acetate (PMA) and 5 ug/ml lipopolysaccharide (LPS) for 24 hours, whole cell lysate. IHC-P: Mouse and rat spleen tissue. ICC/IF: RAW 264.7 treated with 50 nM Phorbol-12-myristate-13-acetate (PMA) and 5 ug/ml lipopolysaccharide (LPS) for 24 hours cells. Flow Cyt (intra): RAW 264.7 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17831 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
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| WB |
1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 30 kDa).
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| IHC-P | (1) |
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Antigen retrieval performed using Universal HIER antigen retrieval reagent (10X) (ab208572). |
| ICC/IF | (1) |
1/500.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/500. |
|
WB
1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 30 kDa). |
|
IHC-P
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Antigen retrieval performed using Universal HIER antigen retrieval reagent (10X) (ab208572). |
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ICC/IF
1/500. |
Target
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Function
Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528).
In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed:21734074). This form is rapidely lost upon angiogenic or proinflammatory activation (PubMed:18787100). -
Tissue specificity
Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta. -
Sequence similarities
Belongs to the IL-1 family. Highly divergent. -
Domain
The homeodomain-like HTH domain mediates nuclear localization and heterochromatin association. -
Post-translational
modificationsThe full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase. -
Cellular localization
Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Associates with heterochromatin and mitotic chromosomes (PubMed:17185418). - Information by UniProt
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Database links
- Entrez Gene: 77125 Mouse
- Entrez Gene: 361749 Rat
- SwissProt: Q8BVZ5 Mouse
- SwissProt: Q66H70 Rat
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Alternative names
- C9orf26 antibody
- CHROMOSOME 9 OPEN READING FRAME 26 antibody
- DKFZp586H0523 antibody
see all
Images
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Western blot - Anti-IL-33 antibody [EPR17831] (ab187060)All lanes : Anti-IL-33 antibody [EPR17831] (ab187060) at 1/1000 dilution
Lane 1 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : RAW 264.7 treated with 50 nM Phorbol-12-myristate-13-acetate (PMA) and 5 ug/ml lipopolysaccharide (LPS) for 24 hours, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Developed using the ECL technique.
Predicted band size: 30 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?Blocking/Dilution: 5% NFDM/TBST
Exposure: 3 minutes
IL-33 expression is induced by LPS treatment of PMA-differentiated RAW 264.7 cells (PMID 19559631; PMID 19933859).
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![Western blot - Anti-IL-33 antibody [EPR17831] (ab187060)](https://www.abcam.com/ps/products/187/ab187060/Images/ab187060-296144-anti-il-33-antibody-epr17831-western-blot.jpg)
Western blot - Anti-IL-33 antibody [EPR17831] (ab187060)All lanes : Anti-IL-33 antibody [EPR17831] (ab187060) at 1/1000 dilution
Lane 1 : Rat lung tissue lysate at 20 µg
Lane 2 : Mouse lung tissue lysate at 10 µg
Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 30 kDa
Observed band size: 30 kDaBlocking/Dilution: 5% NFDM/TBST
Exposure: 3 minutes
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![Flow Cytometry (Intracellular) - Anti-IL-33 antibody [EPR17831] (ab187060)](https://www.abcam.com/ps/products/187/ab187060/Images/ab187060-330718-anti-il-33-antibody-epr17831-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-IL-33 antibody [EPR17831] (ab187060)Intracellular Flow Cytometry analysis ofRAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 50nM PMA and 5µg/ml LPS for 24h (Red) / Untreated control (Green) labeling IL-33 with ab187060 at 1/500 dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody. Cells were fixed with4% paraformaldehyde and permeabilised with0.1% Tween-20.Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR17831] (ab187060)](https://www.abcam.com/ps/products/187/ab187060/Images/ab187060-296145-anti-il-33-antibody-epr17831-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR17831] (ab187060)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling IL33 with ab187060 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use. Nuclear staining in endothelial cells of mouse spleen is observed (PMID: 12819012). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR17831] (ab187060)](https://www.abcam.com/ps/products/187/ab187060/Images/ab187060-296146-anti-il-33-antibody-epr17831-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-33 antibody [EPR17831] (ab187060)Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling IL33 with ab187060 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use. Nuclear staining in endothelial cells of rat spleen is observed (PMID: 12819012). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), ready to use.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-IL-33 antibody [EPR17831] (ab187060)](https://www.abcam.com/ps/products/187/ab187060/Images/ab187060-296147-anti-il-33-antibody-epr17831-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IL-33 antibody [EPR17831] (ab187060)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling IL33 with ab187060 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing increased nuclear staining in RAW 264.7 cells treated with 50 nM Phorbol-12-myristate-13-acetate (PMA) and 5 µg/ml Lipopolysaccharide for 24h.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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![Anti-IL-33 antibody [EPR17831] (ab187060)](https://www.abcam.com/ps/products/187/ab187060/Images/ab187060-7-benefits-of-recombinant-antibodies.png)
Anti-IL-33 antibody [EPR17831] (ab187060)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (5)
ab187060 has been referenced in 5 publications.
- Griffin DR et al. Activating an adaptive immune response from a hydrogel scaffold imparts regenerative wound healing. Nat Mater 20:560-569 (2021). PubMed: 33168979
- Ma Q et al. IL-33/ST2 axis deficiency exacerbates neutrophil-dominant allergic airway inflammation. Clin Transl Immunology 10:e1300 (2021). PubMed: 34178329
- Zhang K et al. Methionine enkephalin (MENK) regulates the immune pathogenesis of type 2 diabetes mellitus via the IL-33/ST2 pathway. Int Immunopharmacol 73:23-40 (2019). PubMed: 31078923
- Yang J et al. LncRNA ANRIL knockdown relieves myocardial cell apoptosis in acute myocardial infarction by regulating IL-33/ST2. Cell Cycle 18:3393-3403 (2019). PubMed: 31674275
- Liang Y et al. Elevated IL-33 promotes expression of MMP2 and MMP9 via activating STAT3 in alveolar macrophages during LPS-induced acute lung injury. Cell Mol Biol Lett 23:52 (2018). PubMed: 30410547