Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)](https://www.abcam.com/ps/products/9/ab9324/Images/ab9324-454107-anti-il-6-antibody-immunohistochemistry-liver-tumor-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)")
Key features and details
- Mouse monoclonal [1.2-2B11-2G10] to IL-6
- Suitable for: IHC-P, Sandwich ELISA, WB
- Reacts with: Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-IL-6 antibody [1.2-2B11-2G10]
See all IL-6 primary antibodies -
Description
Mouse monoclonal [1.2-2B11-2G10] to IL-6 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, Sandwich ELISA, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
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Positive control
- IHC-P: Human liver tumor and prostate tumor tissues; WB: Human spleen and lung tissue lysates, Rat spleen and lung tissue lysates, Recombinant human IL6 protein (ab9627).
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General notes
Although some customers have been successful in IHC we no longer batch test in this application.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 500µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
1.2-2B11-2G10 -
Isotype
IgG2a -
Research areas
Associated products
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ELISA pair antibody
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (11) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Sandwich ELISA |
Use at an assay dependent concentration.
In a sandwich ELISA (assuming 100μl/well), a concentration of 1.0-2.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Human IL-6 when used with ab271236 detection antibody at a concentration of approximately 0.10-0.20 μg/ml. |
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| WB | (3) |
Use at an assay dependent concentration.
To detect human IL-6 by Western blot analysis this antibody can be used at a concentration of 0.50-1.0 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant human IL-6 is 1.0-2.0 ng/lane, under reducing conditions and non-reducing conditions. |
| Notes |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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Sandwich ELISA
Use at an assay dependent concentration. In a sandwich ELISA (assuming 100μl/well), a concentration of 1.0-2.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Human IL-6 when used with ab271236 detection antibody at a concentration of approximately 0.10-0.20 μg/ml. |
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WB
Use at an assay dependent concentration. To detect human IL-6 by Western blot analysis this antibody can be used at a concentration of 0.50-1.0 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant human IL-6 is 1.0-2.0 ng/lane, under reducing conditions and non-reducing conditions. |
Target
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Function
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
Involvement in disease
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3569 Human
- Entrez Gene: 24498 Rat
- Omim: 147620 Human
- SwissProt: P05231 Human
- SwissProt: P20607 Rat
- Unigene: 654458 Human
- Unigene: 9873 Rat
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Alternative names
- Interleukin BSF 2 antibody
- B cell differentiation factor antibody
- B cell stimulatory factor 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tumor tissue labelling IL-6 with ab9324 at 1 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
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![Western blot - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)](https://www.abcam.com/ps/products/9/ab9324/Images/ab9324-251202-anti-il-6-antibody-western-blot.jpg)
Western blot - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)All lanes : Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324) at 1/2000 dilution
Lane 1 : Human Spleen
Lane 2 : Human Lung
Lane 3 : Rat Spleen
Lane 4 : Rat Lung
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : 800CW Goat Anti-Mouse IgG at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 17,25 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa (possible multimer)This antibody was raised against an immunogen that is predicted to recognize the glycosylated form of IL6 as well as the IL6delta4 splice variant. The predicted molecular weights are 25 kDa and 17 kDa respectively. The band observed at 50 kDa may represent multimers of IL6 as reported in the literature.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)](https://www.abcam.com/ps/products/9/ab9324/Images/ab9324-454110-anti-il-6-antibody-immunohistochemistry-prostate-tumor-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tumor tissue labelling IL-6 with ab9324 at 1 µg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.
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![Sandwich ELISA - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)](https://www.abcam.com/ps/products/9/ab9324/Images/ab9324-400102-Western-blot--AntiIL6-antibody-ab9324.png)
Sandwich ELISA - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)ab9324 detecting IL-6 by sandwich ELISA.
Datasheets and documents
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SDS download
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Datasheet download
References (265)
ab9324 has been referenced in 265 publications.
- Ye T et al. Large extracellular vesicles secreted by human iPSC-derived MSCs ameliorate tendinopathy via regulating macrophage heterogeneity. Bioact Mater 21:194-208 (2023). PubMed: 36101856
- Wang XK et al. TNAP-a potential cytokine in the cerebral inflammation in spastic cerebral palsy. Front Mol Neurosci 15:926791 (2022). PubMed: 36187348
- Chen Y et al. Chinese herbal formula (GCNY)-medicated serum alleviates peroxidation induced by H2O2 in human microglial cells. Front Neurosci 16:990040 (2022). PubMed: 36188472
- Liu L et al. Hepatitis B virus promotes hepatocellular carcinoma development by activating GP73 to repress the innate immune response. Infect Agent Cancer 17:52 (2022). PubMed: 36195933
- Tang D et al. Network pharmacology-based prediction of active compounds in the Wenyang Jiedu Huayu formula acting on acute-on-chronic liver failure with experimental support in vitro and in vivo. Front Pharmacol 13:1003479 (2022). PubMed: 36339606