Recombinant Anti-IL-6R antibody [EPR26370-132] (ab300581)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26370-132] to IL-6R
- Suitable for: WB, IHC-P
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-IL-6R antibody [EPR26370-132]
See all IL-6R primary antibodies -
Description
Rabbit monoclonal [EPR26370-132] to IL-6R -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr or IP -
Species reactivity
Reacts with: Mouse
Does not react with: Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate. Untreated mouse spleen tissue lysate, Mouse spleen tissue lysate treated with Protein Deglycosylation Mix II. IHC: Mouse thymus and spleen.
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General notes
ab300581 does not react in IHC-Fr, immunocytochemistry, flow cytometry and immunoprecipitation.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR26370-132 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab300581 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000.
|
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IHC-P |
1/500.
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Notes |
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WB
1/1000. |
IHC-P
1/500. |
Target
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Function
Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis.
Low concentration of a soluble form of IL6 receptor acts as an agonist of IL6 activity. -
Tissue specificity
Isoform 2 is expressed in peripheral blood mononuclear cells and weakly found in urine and serum. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 3 subfamily.
Contains 1 fibronectin type-III domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Domain
The two fibronectin type-III-like domains, contained in the N-terminal part, form together a cytokine-binding domain.
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. -
Post-translational
modificationsA short soluble form may also be released from the membrane by proteolysis. -
Cellular localization
Secreted and Basolateral cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 16194 Mouse
- SwissProt: P22272 Mouse
- Unigene: 2856 Mouse
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Alternative names
- CD 126 antibody
- CD126 antibody
- CD126 antigen antibody
see all
Images
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All lanes : Anti-IL-6R antibody [EPR26370-132] (ab300581) at 1/1000 dilution
Lane 1 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 2 : EHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 70-80 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: WEHI-231(PMID: 2112585)
The identity of the lower MW band at approximately 40 kDa (in lane 1) is unknown. -
All lanes : Anti-IL-6R antibody [EPR26370-132] (ab300581) at 1/1000 dilution
Lane 1 : Untreated mouse spleen tissue lysate
Lane 2 : Mouse spleen tissue lysate treated with Protein Deglycosylation Mix II
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 55, 70-80 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Target is a glycoprotein of approximately 75 kDa and detected as a 55kDa band after treated with Protein Deglycosylation MIX II.
Exposure time: 180 seconds.
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Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling Interleukin-6 receptor subunit alpha with ab300581 at 1/500 dilution (5.04 µg/mL) followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse thymus is observed. The section was incubated with ab300581 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Interleukin-6 receptor subunit alpha with ab300581 at 1/500 dilution (5.04 µg/mL) followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse spleen is observed (PMID: 23378927). The section was incubated with ab300581 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead od primary antibody, followed by a ready to use secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab300581 has been referenced in 1 publication.
- Cui L et al. Circadian gene Rev-erbα influenced by sleep conduces to pregnancy by promoting endometrial decidualization via IL-6-PR-C/EBPβ axis. J Biomed Sci 29:101 (2022). PubMed: 36419076