Recombinant Anti-IL22 RA2/IL-22BP antibody [EPRR23006-70] (ab267467)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL22 RA2/IL-22BP antibody [EPRR23006-70] (ab267467)](https://www.abcam.com/ps/products/267/ab267467/Images/ab267467-1-anti-il22ra2-il22bp-antibody-epr23006-70-immunohistochemistry-tonsil-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL22 RA2/IL-22BP antibody [EPRR23006-70] (ab267467)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPRR23006-70] to IL22 RA2/IL-22BP
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IL22 RA2/IL-22BP antibody [EPRR23006-70]
See all IL22 RA2/IL-22BP primary antibodies -
Description
Rabbit monoclonal [EPRR23006-70] to IL22 RA2/IL-22BP -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human tonsil, Human colon, Human carcinoma and Human liver tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Preadsorbed IgG fraction -
Clonality
Monoclonal -
Clone number
EPRR23006-70 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab267467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Isoform 2 is a receptor for IL22. Binds to IL22, prevents interaction with the functional IL-22R complex and blocks the activity of IL22 (in vitro). May play an important role as an IL22 antagonist in the regulation of inflammatory responses.
Isoform 1 may play a role in establishing and maintaining successful pregnancy. -
Tissue specificity
Expressed in placenta, spleen, breast, skin and lung. Also detected in intestinal tract, testis, brain, heart and thymus. No expression found in prostate, bladder, kidney, ovary, muscle, bone marrow, liver and uterus. Isoform 1 is expressed only in placenta. Isoform 2 is expressed in placenta and breast and at lower level in spleen, skin, thymus and stomach. -
Sequence similarities
Belongs to the type II cytokine receptor family.
Contains 3 fibronectin type-III domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 116379 Human
- Omim: 606648 Human
- SwissProt: Q969J5 Human
- Unigene: 126891 Human
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Alternative names
- IL 22BP antibody
- IL 22RA2 antibody
- IL22RA2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL22 RA2/IL-22BP antibody [EPRR23006-70] (ab267467)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling IL22 RA2/IL-22BP with ab267467 at 1/500 dilution (1.09 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil (PMID: 28512157) is observed. The section was incubated with ab267467 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL22 RA2/IL-22BP antibody [EPRR23006-70] (ab267467)](https://www.abcam.com/ps/products/267/ab267467/Images/ab267467-3-anti-il22ra2-il22bp-antibody-epr23006-70-immunohistochemistry-colon-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL22 RA2/IL-22BP antibody [EPRR23006-70] (ab267467)Immunohistochemical analysis of paraffin-embedded Human carcinoma tissue labeling IL22 RA2/IL-22BP with ab267467 at 1/500 dilution (1.09 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells in human carcinoma (PMID: 26329427) is observed. The section was incubated with ab267467 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL22 RA2/IL-22BP antibody [EPRR23006-70] (ab267467)](https://www.abcam.com/ps/products/267/ab267467/Images/ab267467-2-anti-il22ra2-il22bp-antibody-epr23006-70-immunohistochemistry-colon-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL22 RA2/IL-22BP antibody [EPRR23006-70] (ab267467)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling IL22 RA2/IL-22BP with ab267467 at 1/500 dilution (1.09 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells in human colon (PMID: 26329427) is observed. The section was incubated with ab267467 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL22 RA2/IL-22BP antibody [EPRR23006-70] (ab267467)](https://www.abcam.com/ps/products/267/ab267467/Images/ab267467-4-anti-il22ra2-il22bp-antibody-epr23006-70-immunohistochemistry-liver-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL22 RA2/IL-22BP antibody [EPRR23006-70] (ab267467)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling IL22 RA2/IL-22BP with ab267467 at 1/500 dilution (1.09 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: no staining on human liver (PMID: 21674558, PMID: 23653115) is observed. The section was incubated with ab267467 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Anti-IL22 RA2/IL-22BP antibody [EPRR23006-70] (ab267467)](https://www.abcam.com/ps/products/267/ab267467/Images/ab267467-5-benefits-of-recombinant-antibodies.png)
Anti-IL22 RA2/IL-22BP antibody [EPRR23006-70] (ab267467)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab267467 has not yet been referenced specifically in any publications.