Anti-ILP2 antibody (ab9664)
Key features and details
- Rabbit polyclonal to ILP2
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-ILP2 antibody -
Description
Rabbit polyclonal to ILP2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
TGYEARLITFGT
, corresponding to amino acids 2-13 of Human ILP2. (Peptide available as ab9665.) -
Positive control
- WB: HepG2 and MOLT4 cell lysate. ICC: HepG2 cells
-
General notes
Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family including IAP, XIAP/ILP-1/MIHA, and Livin/KIAP that bind to and inhibits specific proteases. A novel member in the IAP protein family was recently identified and designated ILP-2 for IAP-like protein-2 (1). ILP-2 has high homology to ILP-1, but is encoded by a distinct gene that is solely expressed in testis of tested normal human tissues (1). ILP-2, unlike ILP-1, has no inhibitory effect on Fas and TNF induced apoptosis, but potently inhibits apoptosis induced by overexpression of Bax or by coexpression of caspase-9 with Apaf-1. ILP-2 interacts with the processed caspase-9. These results suggest that ILP-2 is a novel IAP family member with restricted specificity for caspase-9.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family including IAP, XIAP/ILP-1/MIHA, and Livin/KIAP that bind to and inhibits specific proteases. A novel member in the IAP protein family was recently identified and designated ILP-2 for IAP-like protein-2 (1). ILP-2 has high homology to ILP-1, but is encoded by a distinct gene that is solely expressed in testis of tested normal human tissues (1). ILP-2, unlike ILP-1, has no inhibitory effect on Fas and TNF induced apoptosis, but potently inhibits apoptosis induced by overexpression of Bax or by coexpression of caspase-9 with Apaf-1. ILP-2 interacts with the processed caspase-9. These results suggest that ILP-2 is a novel IAP family member with restricted specificity for caspase-9. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9664 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 33 kDa.
|
|
| ICC |
Use a concentration of 10 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 33 kDa. |
|
ICC
Use a concentration of 10 µg/ml. |
Target
-
Function
Protects against apoptosis mediated by BAX. -
Tissue specificity
Testis specific in normal tissues. -
Sequence similarities
Belongs to the IAP family.
Contains 1 BIR repeat.
Contains 1 RING-type zinc finger. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 112401 Human
- SwissProt: Q96P09 Human
- Unigene: 348263 Human
-
Alternative names
- Baculoviral IAP repeat containing 8 antibody
- Baculoviral IAP repeat containing protein 8 antibody
- Baculoviral IAP repeat-containing protein 8 antibody
see all
Images
-
Western blot - Anti-ILP2 antibody (ab9664)All lanes : Anti-ILP2 antibody (ab9664) at 1 µg/ml
Lane 1 : HepG2 cell lysate
Lane 2 : MOLT4 cell lysate -
Immunocytochemistry - Anti-ILP2 antibody (ab9664)ab9664 at 10µg/ml staining ILP2 in HepG2 cells by Immunocytochemistry.
Datasheets and documents
-
Datasheet download
References (6)
ab9664 has been referenced in 6 publications.
- Wang F et al. Deficient spermiogenesis in mice lacking Rlim. Elife 10:N/A (2021). PubMed: 33620316
- Mukhtar T et al. Tead transcription factors differentially regulate cortical development. Sci Rep 10:4625 (2020). PubMed: 32170161
- Charish J et al. Neogenin neutralization prevents photoreceptor loss in inherited retinal degeneration. J Clin Invest 130:2054-2068 (2020). PubMed: 32175920
- Jaén RI et al. BML-111 treatment prevents cardiac apoptosis and oxidative stress in a mouse model of autoimmune myocarditis. FASEB J 34:10531-10546 (2020). PubMed: 32543747
- Kong D et al. Protective effects of Asiatic acid against pelvic inflammatory disease in rats. Exp Ther Med 17:4687-4692 (2019). PubMed: 31086602
- Zhu L et al. Inhibitor of apoptosis protein-like protein-2: A novel growth accelerator for breast cancer cells. Oncol Rep 40:2047-2055 (2018). PubMed: 30106449
