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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-IMP3 antibody [EPR12021-114] (ab179807)

Submit a review Submit a question References (2)
Western blot - Anti-IMP3 antibody [EPR12021-114] (ab179807)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (ab179807)
  • Western blot - Anti-IMP3 antibody [EPR12021-114] (ab179807)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (ab179807)
  • Western blot - Anti-IMP3 antibody [EPR12021-114] (ab179807)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (ab179807)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (ab179807)
  • Anti-IMP3 antibody [EPR12021-114] (ab179807)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12021-114] to IMP3
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-IMP3 antibody [EPR12021-114]
    See all IMP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR12021-114] to IMP3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human IMP3 aa 1-200. The exact sequence is proprietary.
    Database link: O00425

  • Positive control

    • IHC-P: Human lung cancer and fetal kidney tissues. WB: HeLa, 293T and BxPC-3 cell lysates. HeLa, HEK-293 and BxPC-3 whole cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 63 kDa.

For unpurified use at 1/1000 - 1/5000.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.
Notes
WB
1/1000. Predicted molecular weight: 63 kDa.

For unpurified use at 1/1000 - 1/5000.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.

Target

  • Function

    RNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3'-UTR of CD44 mRNA.

  • Tissue specificity

    Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic and renal-cell carcinomas (at protein level). Expressed ubiquitously during fetal development at 8 and 14 weeks of gestation. Expressed in ovary, testis, brain, placenta, pancreatic cancer tissues and pancreatic cancer cell lines.

  • Sequence similarities

    Belongs to the RRM IMP/VICKZ family.
    Contains 4 KH domains.
    Contains 2 RRM (RNA recognition motif) domains.

  • Domain

    The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA.

  • Cellular localization

    Nucleus. Cytoplasm. Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Localized at the connecting piece and the tail of the spermatozoa. Colocalized with CD44 mRNA in RNP granules.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cancer/testis antigen 98 antibody
      • CT98 antibody
      • DKFZp686F1078 antibody
      • hKOC antibody
      • IF2B3_HUMAN antibody
      • IGF II mRNA binding protein 3 antibody
      • IGF-II mRNA-binding protein 3 antibody
      • IGF2 mRNA binding protein 3 antibody
      • IGF2 mRNA-binding protein 3 antibody
      • IGF2BP3 antibody
      • IMP 3 antibody
      • IMP-3 antibody
      • Insulin like growth factor 2 mRNA binding protein 3 antibody
      • Insulin-like growth factor 2 mRNA-binding protein 3 antibody
      • KH domain containing protein overexpressed in cancer antibody
      • KH domain-containing protein overexpressed in cancer antibody
      • KOC 1 antibody
      • KOC1 antibody
      • VICKZ 3 antibody
      • VICKZ family member 3 antibody
      • VICKZ3 antibody
      see all

    Images

    • Western blot - Anti-IMP3 antibody [EPR12021-114] (ab179807)
      Western blot - Anti-IMP3 antibody [EPR12021-114] (ab179807)
      Lanes 1-2 : Anti-IMP3 antibody [EPR12021-114] (ab179807) at 1/1000 dilution (Purified)
      Lane 3 : Anti-IMP3 antibody [EPR12021-114] (ab179807) at 1/10000 dilution

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
      Lane 3 : BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
      Lane 3 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

      Predicted band size: 63 kDa
      Observed band size: 69 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (ab179807)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (ab179807)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue sections labeling IMP3 with purified ab179807 at 1/1000 dilution (0.10 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Western blot - Anti-IMP3 antibody [EPR12021-114] (ab179807)
      Western blot - Anti-IMP3 antibody [EPR12021-114] (ab179807)

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: IGF2BP3 (IMP3) knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)

      Lanes 1 - 3: Merged signal (red and green). Green - Unpurified ab179807 observed at 70 kDa. Red - loading control, ab9484, observed at 37 kDa.

      Unpurified ab179807 was shown to specifically react with IGF2BP3 (IMP3) in wild-type HAP1 cells as signal was lost in IGF2BP3 (IMP3) knockout cells. Wild-type and IGF2BP3 (IMP3) knockout samples were subjected to SDS-PAGE. Ab179807 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (ab179807)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (ab179807)

      Unpurified ab179807 at 1:100 staining IMP3 antibody in human lung cancer tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling IMP3 with ab179807 at 1/100 dilution. Counter stained with hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Western blot - Anti-IMP3 antibody [EPR12021-114] (ab179807)
      Western blot - Anti-IMP3 antibody [EPR12021-114] (ab179807)
      All lanes : Anti-IMP3 antibody [EPR12021-114] (ab179807) at 1/1000 dilution (unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
      Lane 3 : BxPC-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 63 kDa
      Observed band size: 69 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (ab179807)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (ab179807)

      Unpurified ab179807 at 1:100 staining IMP3 antibody in human fetal kidney tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded human fetal kidney tissue labeling IMP3 with ab179807 at 1/100 dilution. Counter stained with hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (ab179807)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (ab179807)

      Negative control: Immunohistochemical analysis of paraffin-embedded human adult kidney tissue labeling IMP3 with unpurified ab179807 at 1/100 dilution. Counter stained with hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Anti-IMP3 antibody [EPR12021-114] (ab179807)
      Anti-IMP3 antibody [EPR12021-114] (ab179807)

    References (2)

    Publishing research using ab179807? Please let us know so that we can cite the reference in this datasheet.

    ab179807 has been referenced in 2 publications.

    • Yang Y  et al. Dynamic Patterns of N6-Methyladenosine Profiles of Messenger RNA Correlated with the Cardiomyocyte Regenerability during the Early Heart Development in Mice. Oxid Med Cell Longev 2021:5537804 (2021). PubMed: 34413927
    • Zhang X  et al. IMP3 accelerates the progression of prostate cancer through inhibiting PTEN expression in a SMURF1-dependent way. J Exp Clin Cancer Res 39:190 (2020). PubMed: 32938489

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