Recombinant Anti-IMP3 antibody [EPR12022] (ab177942)
![Western blot - Anti-IMP3 antibody [EPR12022] (ab177942)](https://www.abcam.com/ps/products/177/ab177942/Images/ab177942-286146-anti-imp3-antibody-epr12022-western-blot.jpg "Western blot - Anti-IMP3 antibody [EPR12022] (ab177942)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12022] to IMP3
- Suitable for: WB, ICC/IF, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IMP3 antibody [EPR12022]
See all IMP3 primary antibodies -
Description
Rabbit monoclonal [EPR12022] to IMP3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Recombinant fragment within Human IMP3. The exact sequence is proprietary.
Database link: O00425 -
Positive control
- HeLa, 293T, K562 and HT-29 cell lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12022 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab177942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 69 kDa (predicted molecular weight: 63 kDa).
|
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| ICC/IF |
1/50 - 1/100.
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| IP |
1/10 - 1/100.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 69 kDa (predicted molecular weight: 63 kDa). |
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ICC/IF
1/50 - 1/100. |
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IP
1/10 - 1/100. |
Target
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Function
RNA-binding protein that act as a regulator of mRNA translation and stability. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binds to sequences in the 3'-UTR of CD44 mRNA. -
Tissue specificity
Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary and gonocytes of testis, growing oocytes, spermatogonia and semen (at protein level). Expressed in cervix adenocarcinoma, in testicular, pancreatic and renal-cell carcinomas (at protein level). Expressed ubiquitously during fetal development at 8 and 14 weeks of gestation. Expressed in ovary, testis, brain, placenta, pancreatic cancer tissues and pancreatic cancer cell lines. -
Sequence similarities
Belongs to the RRM IMP/VICKZ family.
Contains 4 KH domains.
Contains 2 RRM (RNA recognition motif) domains. -
Domain
The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA. -
Cellular localization
Nucleus. Cytoplasm. Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Localized at the connecting piece and the tail of the spermatozoa. Colocalized with CD44 mRNA in RNP granules. - Information by UniProt
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Database links
- Entrez Gene: 10643 Human
- Entrez Gene: 312320 Rat
- Omim: 608259 Human
- SwissProt: O00425 Human
- Unigene: 700696 Human
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Alternative names
- Cancer/testis antigen 98 antibody
- CT98 antibody
- DKFZp686F1078 antibody
see all
Images
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Western blot - Anti-IMP3 antibody [EPR12022] (ab177942)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: IGF2BP3 (IMP3) knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: HEK293 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab177942 observed at 70 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab177942 was shown to specifically react with IGF2BP3 (IMP3) in wild-type HAP1 cells as signal was lost in IGF2BP3 (IMP3) knockout cells. Wild-type and IGF2BP3 (IMP3) knockout samples were subjected to SDS-PAGE. Ab177942 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-IMP3 antibody [EPR12022] (ab177942)](https://www.abcam.com/ps/products/177/ab177942/Images/ab177942-205960-anti-imp3-antibody-epr12022-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IMP3 antibody [EPR12022] (ab177942)Immunofluorescent analysis of HeLa cells labeling IMP3 with ab177942 at 1/50 dilution (green). DAPI nuclear staining (blue).
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![Immunoprecipitation - Anti-IMP3 antibody [EPR12022] (ab177942)](https://www.abcam.com/ps/products/177/ab177942/Images/ab177942-205959-anti-imp3-antibody-epr12022-immunoprecipitation.jpg)
Immunoprecipitation - Anti-IMP3 antibody [EPR12022] (ab177942)Western blot analysis on immunoprecipitation pellet from 293T cell lysate using ab177942.
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![Western blot - Anti-IMP3 antibody [EPR12022] (ab177942)](https://www.abcam.com/ps/products/177/ab177942/Images/ab177942-205958-anti-imp3-antibody-epr12022-western-blot.jpg)
Western blot - Anti-IMP3 antibody [EPR12022] (ab177942)All lanes : Anti-IMP3 antibody [EPR12022] (ab177942) at 1/1000 dilution
Lane 1 : Hela cell lysate
Lane 2 : 293T cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HT-29 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 63 kDa
Observed band size: 69 kDa why is the actual band size different from the predicted? -
![Anti-IMP3 antibody [EPR12022] (ab177942)](https://www.abcam.com/ps/products/177/ab177942/Images/ab177942-5-benefits-of-recombinant-antibodies.png)
Anti-IMP3 antibody [EPR12022] (ab177942)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab177942 has been referenced in 1 publication.
- Mizutani R et al. Oncofetal protein IGF2BP3 facilitates the activity of proto-oncogene protein eIF4E through the destabilization of EIF4E-BP2 mRNA. Oncogene 35:3495-502 (2016). PubMed: 26522719