Recombinant Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
![Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)](https://www.abcam.com/ps/products/131/ab131158/Images/ab131158-263736-anti-impdh2-antibody-epr8364b-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8364(B)] to IMPDH2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IMPDH2 antibody [EPR8364(B)]
See all IMPDH2 primary antibodies -
Description
Rabbit monoclonal [EPR8364(B)] to IMPDH2 -
Host species
Rabbit -
Specificity
The immunogen used to raise this antibody shares 79% homology with Human IMPDH1. Cross-reactivity with this protein has not been confirmed experimentally. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human IMPDH2 aa 100-200. The exact sequence is proprietary.
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Positive control
- K562, HepG2, HeLa and NCI-H460 cell lysates; Human colon tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.20 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8364(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
|
|
| IHC-P | (1) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/250.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 56 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/250. |
Target
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Function
Rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. -
Tissue specificity
IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor. -
Pathway
Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1. -
Sequence similarities
Belongs to the IMPDH/GMPR family.
Contains 2 CBS domains. -
Post-translational
modificationsThe N-terminus is blocked. - Information by UniProt
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Database links
- Entrez Gene: 3615 Human
- Entrez Gene: 23918 Mouse
- Entrez Gene: 301005 Rat
- Omim: 146691 Human
- SwissProt: P12268 Human
- SwissProt: P24547 Mouse
- SwissProt: E9PU28 Rat
- Unigene: 654400 Human
see all -
Alternative names
- IMDH2_HUMAN antibody
- IMP (inosine monophosphate) dehydrogenase 2 antibody
- IMP dehydrogenase 2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
ab131158 staining IMPDH2 in K562 (human chronic myelogenous leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.
DAPI was used as a nuclear counterstain and the negative control was PBS only.
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![Western blot - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)](https://www.abcam.com/ps/products/131/ab131158/Images/ab131158-145428-anti-impdh2-antibody-epr8364b-western-blot.jpg)
Western blot - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)All lanes : Anti-IMPDH2 antibody [EPR8364(B)] (ab131158) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : NCI-H460 cell lysate
Predicted band size: 56 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)](https://www.abcam.com/ps/products/131/ab131158/Images/ab131158-145427-anti-impdh2-antibody-epr8364b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling IMPDH2 with ab131158 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)](https://www.abcam.com/ps/products/131/ab131158/Images/ab131158-145426-anti-impdh2-antibody-epr8364b.jpg)
OI-RD Scanning - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158) -
![Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)](https://www.abcam.com/ps/products/131/ab131158/Images/ab131158-5-benefits-of-recombinant-antibodies.png)
Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab131158 has been referenced in 12 publications.
- Chaiyawat P et al. IMPDH2 and HPRT expression and a prognostic significance in preoperative and postoperative patients with osteosarcoma. Sci Rep 11:10887 (2021). PubMed: 34035425
- Xu H et al. IMPDH2 promotes cell proliferation and epithelial-mesenchymal transition of non-small cell lung cancer by activating the Wnt/ß-catenin signaling pathway. Oncol Lett 20:219 (2020). PubMed: 32963625
- Kofuji S et al. IMP dehydrogenase-2 drives aberrant nucleolar activity and promotes tumorigenesis in glioblastoma. Nat Cell Biol 21:1003-1014 (2019). PubMed: 31371825
- Wolfe K et al. Dynamic compartmentalization of purine nucleotide metabolic enzymes at leading edge in highly motile renal cell carcinoma. Biochem Biophys Res Commun 516:50-56 (2019). PubMed: 31196624
- Ziegler CM et al. NEDD4 family ubiquitin ligases associate with LCMV Z's PPXY domain and are required for virus budding, but not via direct ubiquitination of Z. PLoS Pathog 15:e1008100 (2019). PubMed: 31710650