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RecombinantRabMAb

Recombinant Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)

Reviews (1) Submit a question References (13)
Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
  • Western blot - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
  • Western blot - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
  • OI-RD Scanning - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
  • Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8364(B)] to IMPDH2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

Overview

  • Product name

    Anti-IMPDH2 antibody [EPR8364(B)]
    See all IMPDH2 primary antibodies

  • Description

    Rabbit monoclonal [EPR8364(B)] to IMPDH2

  • Host species

    Rabbit

  • Specificity

    The immunogen used to raise this antibody shares 79% homology with Human IMPDH1.

    This antibody shows very faint band when testing IMPDH1 recombinant protein in WB test.

     

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human IMPDH2 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • K562, HepG2, HeLa and NCI-H460 cell lysates; Human colon tissue

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab131158 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IHC-P (1)
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.

Target

  • Function

    Rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors.

  • Tissue specificity

    IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.

  • Pathway

    Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.

  • Sequence similarities

    Belongs to the IMPDH/GMPR family.
    Contains 2 CBS domains.

  • Post-translational
    modifications

    The N-terminus is blocked.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • IMDH2_HUMAN antibody
    • IMP (inosine monophosphate) dehydrogenase 2 antibody
    • IMP dehydrogenase 2 antibody
    • IMP oxireductase 2 antibody
    • IMPD 2 antibody
    • IMPD2 antibody
    • IMPDH 2 antibody
    • IMPDH II antibody
    • IMPDH-II antibody
    • Impdh2 antibody
    • Inosine 5' monophosphate dehydrogenase 2 antibody
    • Inosine 5' phosphate dehydrogenase 2 antibody
    • Inosine monophosphate dehydrogenase type II antibody
    • Inosine-5''-monophosphate dehydrogenase 2 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
    Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)

    ab131158 staining IMPDH2 in K562 (human chronic myelogenous leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.

    DAPI was used as a nuclear counterstain and the negative control was PBS only.

  • Western blot - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
    Western blot - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
    All lanes : Anti-IMPDH2 antibody [EPR8364(B)] (ab131158) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : NCI-H460 cell lysate

    Predicted band size: 56 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling IMPDH2 with ab131158 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
    Western blot - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
    All lanes : Anti-IMPDH2 antibody [EPR8364(B)] (ab131158) at 1/1000 dilution

    Lanes 1 & 3 : C-Myc/DDK tagged full length IMPDH1 Human Recombinant Protein
    Lane 2 : C-Myc/DDK tagged full length IMPDH2 Human Recombinant Protein

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 56 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.


    Exposure times:

    Lane 1&2: 5 seconds

    Lane 3: 180 seconds.

    This antibody shows very faint band when testing IMPDH1 recombinant protein in WB test

  • OI-RD Scanning - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
    OI-RD Scanning - Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)
    Anti-IMPDH2 antibody [EPR8364(B)] (ab131158)

References (13)

Publishing research using ab131158? Please let us know so that we can cite the reference in this datasheet.

ab131158 has been referenced in 13 publications.

  • He S  et al. A recombinant scFv antibody-based fusion protein that targets EGFR associated with IMPDH2 downregulation and its drug conjugate show therapeutic efficacy against esophageal cancer. Drug Deliv 29:1243-1256 (2022). PubMed: 35416106
  • Chaiyawat P  et al. IMPDH2 and HPRT expression and a prognostic significance in preoperative and postoperative patients with osteosarcoma. Sci Rep 11:10887 (2021). PubMed: 34035425
  • Xu H  et al. IMPDH2 promotes cell proliferation and epithelial-mesenchymal transition of non-small cell lung cancer by activating the Wnt/ß-catenin signaling pathway. Oncol Lett 20:219 (2020). PubMed: 32963625
  • Kofuji S  et al. IMP dehydrogenase-2 drives aberrant nucleolar activity and promotes tumorigenesis in glioblastoma. Nat Cell Biol 21:1003-1014 (2019). PubMed: 31371825
  • Wolfe K  et al. Dynamic compartmentalization of purine nucleotide metabolic enzymes at leading edge in highly motile renal cell carcinoma. Biochem Biophys Res Commun 516:50-56 (2019). PubMed: 31196624
View all Publications for this product

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-IMPDH2 antibody [EPR8364(B)]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human cancer xenograft)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH9
Permeabilization
No
Specification
Human cancer xenograft
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Jun 29 2015

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