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RecombinantRabMAb

Recombinant Anti-IMPDH2 antibody [EPR8365(B)] (ab129165)

Submit a review Submit a question References (3)
Western blot - Anti-IMPDH2 antibody [EPR8365(B)] (ab129165)
  • Flow Cytometry (Intracellular) - Anti-IMPDH2 antibody [EPR8365(B)] (ab129165)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR8365(B)] (ab129165)
  • Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR8365(B)] (ab129165)
  • OI-RD Scanning - Anti-IMPDH2 antibody [EPR8365(B)] (ab129165)
  • Anti-IMPDH2 antibody [EPR8365(B)] (ab129165)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8365(B)] to IMPDH2
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

APC Carrier Free HRP PE

Overview

  • Product name

    Anti-IMPDH2 antibody [EPR8365(B)]
    See all IMPDH2 primary antibodies

  • Description

    Rabbit monoclonal [EPR8365(B)] to IMPDH2

  • Host species

    Rabbit

  • Specificity

    The immunogen used to raise this antibody shares 86% homology with Human IMPDH1. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human IMPDH2 aa 500-600 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HepG2, A549, MCF7, HeLa and K562 cell lysates; Human breast carcinoma and kidney tissues; MCF7 cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Notes
Flow Cyt (Intra)
1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Application notes
Is unsuitable for IP.

Target

  • Function

    Rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors.

  • Tissue specificity

    IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor.

  • Pathway

    Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.

  • Sequence similarities

    Belongs to the IMPDH/GMPR family.
    Contains 2 CBS domains.

  • Post-translational
    modifications

    The N-terminus is blocked.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • IMDH2_HUMAN antibody
    • IMP (inosine monophosphate) dehydrogenase 2 antibody
    • IMP dehydrogenase 2 antibody
    • IMP oxireductase 2 antibody
    • IMPD 2 antibody
    • IMPD2 antibody
    • IMPDH 2 antibody
    • IMPDH II antibody
    • IMPDH-II antibody
    • Impdh2 antibody
    • Inosine 5' monophosphate dehydrogenase 2 antibody
    • Inosine 5' phosphate dehydrogenase 2 antibody
    • Inosine monophosphate dehydrogenase type II antibody
    • Inosine-5''-monophosphate dehydrogenase 2 antibody
    see all

Images

  • Western blot - Anti-IMPDH2 antibody [EPR8365(B)] (ab129165)
    Western blot - Anti-IMPDH2 antibody [EPR8365(B)] (ab129165)
    All lanes : Anti-IMPDH2 antibody [EPR8365(B)] (ab129165) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 56 kDa
    Observed band size: 56 kDa

  • Flow Cytometry (Intracellular) - Anti-IMPDH2 antibody [EPR8365(B)] (ab129165)
    Flow Cytometry (Intracellular) - Anti-IMPDH2 antibody [EPR8365(B)] (ab129165)

    Overlay histogram showing K562 cells stained with ab129165 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab129165, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR8365(B)] (ab129165)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPDH2 antibody [EPR8365(B)] (ab129165)

    ab129165, at 1/250 dilution, staining IMPDH2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR8365(B)] (ab129165)
    Immunocytochemistry/ Immunofluorescence - Anti-IMPDH2 antibody [EPR8365(B)] (ab129165)
    ab129165, at 1/250 dilution, staining IMPDH2 in MCF7 cells by Immunofluorescence.
  • OI-RD Scanning - Anti-IMPDH2 antibody [EPR8365(B)] (ab129165)
    OI-RD Scanning - Anti-IMPDH2 antibody [EPR8365(B)] (ab129165)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-IMPDH2 antibody [EPR8365(B)] (ab129165)
    Anti-IMPDH2 antibody [EPR8365(B)] (ab129165)

References (3)

Publishing research using ab129165? Please let us know so that we can cite the reference in this datasheet.

ab129165 has been referenced in 3 publications.

  • Duong-Ly KC  et al. T cell activation triggers reversible inosine-5'-monophosphate dehydrogenase assembly. J Cell Sci 131:N/A (2018). PubMed: 30154209
  • Anthony SA  et al. Reconstituted IMPDH polymers accommodate both catalytically active and inactive conformations. Mol Biol Cell N/A:N/A (2017). WB ; Human . PubMed: 28794265
  • Barfeld SJ  et al. Myc-dependent purine biosynthesis affects nucleolar stress and therapy response in prostate cancer. Oncotarget 6:12587-602 (2015). IHC-P ; Human . PubMed: 25869206

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