Anti-IMPG1 antibody (ab113493)
Key features and details
- Rabbit polyclonal to IMPG1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IMPG1 antibody -
Description
Rabbit polyclonal to IMPG1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment, corresponding to amino acids 217-437 of Human IMPG1 (BC117450).
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Positive control
- Human fetal liver, fetal muscle, Fetal kidney, HepG2, 97 H, and LO2 cell lysates; Human fetal liver tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary. -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab113493 is purified by a peptide affinity column. -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 89 kDa.
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| IHC-P |
1/100 - 1/500.
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| Notes |
|---|
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WB
1/500 - 1/1000. Predicted molecular weight: 89 kDa. |
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IHC-P
1/100 - 1/500. |
Target
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Function
May interact with hyaluronan which may serve to form a basic macromolecular scaffold comprising the insoluble interphotoreceptor matrix. -
Tissue specificity
Abundantly expressed in retina where it is specifically expressed by cone and rod photoreceptor cells. Localizes to cone and rod photoreceptor cells surrounding the interphotoreceptor matrix of the retina. -
Sequence similarities
Contains 2 SEA domains. -
Post-translational
modificationsThe N-terminus is blocked.
Highly glycosylated (N- and O-linked carbohydrates and sialic acid). -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3617 Human
- Entrez Gene: 63859 Mouse
- Entrez Gene: 66014 Rat
- Omim: 602870 Human
- SwissProt: Q17R60 Human
- SwissProt: Q8R1W8 Mouse
- SwissProt: Q9ET62 Rat
- Unigene: 590893 Human
see all -
Alternative names
- IPM150 antibody
- GP147 antibody
- IMPG1 antibody
see all
Images
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Western blot - Anti-IMPG1 antibody (ab113493)All lanes : Anti-IMPG1 antibody (ab113493) at 1/500 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal muscle lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : HepG2 cell lysate
Lane 5 : 97 H cell lysate
Lane 6 : LO2 cell lysate
Predicted band size: 89 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMPG1 antibody (ab113493)ab113493 at 1/100 dilution staining IMPG1 in Formalin-fixed, Paraffin-embedded Human fetal liver tissue by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab113493 has been referenced in 1 publication.
- Felemban M et al. Extracellular matrix component expression in human pluripotent stem cell-derived retinal organoids recapitulates retinogenesis in vivo and reveals an important role for IMPG1 and CD44 in the development of photoreceptors and interphotoreceptor matrix. Acta Biomater 74:207-221 (2018). PubMed: 29777959