Recombinant Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)
![Western blot - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)](https://www.abcam.com/ps/products/181/ab181046/Images/ab181046-393580-anti-importin4imp4-antibody-epr13660-western-blot-human-knockout-lysate.jpg "Western blot - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13660] to Importin4/Imp4
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Importin4/Imp4 antibody [EPR13660]
See all Importin4/Imp4 primary antibodies -
Description
Rabbit monoclonal [EPR13660] to Importin4/Imp4 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment within Human Importin4/Imp4. The exact sequence is proprietary.
Database link: Q8TEX9 -
Positive control
- WB: HepG2, HeLa, A549 and Human seminoma lysates. IHC-P: Human breast carcinoma tissue and Human testis tissue. ICC/IF: A549 cells. Flow Cyt (intra): Permeabilized HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13660 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Perform heat mediated antigen retrieval, heat up to 98°C, below boiling, and then let cool for 10-20 min, before commencing with IHC staining. |
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| WB |
1/10000 - 1/50000. Predicted molecular weight: 119 kDa.
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| ICC/IF |
1/50 - 1/100.
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| IP |
1/10 - 1/100.
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| Notes |
|---|
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Perform heat mediated antigen retrieval, heat up to 98°C, below boiling, and then let cool for 10-20 min, before commencing with IHC staining. |
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WB
1/10000 - 1/50000. Predicted molecular weight: 119 kDa. |
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ICC/IF
1/50 - 1/100. |
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IP
1/10 - 1/100. |
Target
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Function
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of RPS3A. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. -
Sequence similarities
Belongs to the importin beta family.
Contains 6 HEAT repeats.
Contains 1 importin N-terminal domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 79711 Human
- Entrez Gene: 75751 Mouse
- Entrez Gene: 290228 Rat
- SwissProt: Q8TEX9 Human
- SwissProt: Q8VI75 Mouse
- Unigene: 411865 Human
- Unigene: 27546 Mouse
- Unigene: 474981 Mouse
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Alternative names
- Imp4 antibody
- Imp4b antibody
- Importin 4 antibody
see all
Images
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Western blot - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)All lanes : Anti-Importin4/Imp4 antibody [EPR13660] (ab181046) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : IPO4 knockout HEK293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 119 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab181046 observed at 130 kDa. Red - loading control ab8245 observed at 36 kDa.
ab181046 Anti-Importin4/Imp4 antibody [EPR13660] was shown to specifically react with Importin4/Imp4 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266175 (knockout cell lysate ab258005) was used. Wild-type and Importin4/Imp4 knockout samples were subjected to SDS-PAGE. ab181046 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)](https://www.abcam.com/ps/products/181/ab181046/Images/ab181046-212091-anti-importin4-antibody-epr13660-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Importin4/Imp4 using ab181046 at a 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)](https://www.abcam.com/ps/products/181/ab181046/Images/ab181046-212090-anti-importin4-antibody-epr13660-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Importin4/Imp4 using ab181046 at a 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)](https://www.abcam.com/ps/products/181/ab181046/Images/ab181046-212092-anti-importin4-antibody-epr13660-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)Immunofluorescent staining of A549 cells labeling Importin4/Imp4 using ab181046 at a 1/50 dilution (red) and DAPI nuclear staining (blue).
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![Flow Cytometry (Intracellular) - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)](https://www.abcam.com/ps/products/181/ab181046/Images/ab181046-212093-anti-importin4-antibody-epr13660-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)Intracellular flow cytometric analysis of permeabilized HepG2 cells labeling Importin4/Imp4 using ab181046 at a 1/10 dilution (red) or a rabbit IgG control (green).
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![Western blot - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)](https://www.abcam.com/ps/products/181/ab181046/Images/ab181046-212089-anti-importin4-antibody-epr13660-western-blot.jpg)
Western blot - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)All lanes : Anti-Importin4/Imp4 antibody [EPR13660] (ab181046) at 1/100000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Human seminoma lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 119 kDa -
![Immunoprecipitation - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)](https://www.abcam.com/ps/products/181/ab181046/Images/ab181046-212094-anti-importin4-antibody-epr13660-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate labelling Importin4/Imp4 immunoprecipitated using ab181046 at a 1/10 dilution.
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![Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)](https://www.abcam.com/ps/products/181/ab181046/Images/ab181046-8-benefits-of-recombinant-antibodies.png)
Anti-Importin4/Imp4 antibody [EPR13660] (ab181046)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab181046 has been referenced in 2 publications.
- Li R et al. IMP4 Silencing Inhibits the Malignancy of Lung Adenocarcinoma via ERK Pathway. J Oncol 2022:8545441 (2022). PubMed: 36317123
- Kalita J et al. On the asymmetric partitioning of nucleocytoplasmic transport - recent insights and open questions. J Cell Sci 134:N/A (2021). PubMed: 33912945