Recombinant Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)
![Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)](https://www.abcam.com/ps/products/76/ab76157/Images/ab76157-469658-anti-indoleamine-2-3-dioxygenase-antibody-epr1230y-western-blot-wildtype-a549-vehicle-control-ifn-gamma-0-ngml-48-h-wildtype-a549-trea.jpg "Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1230Y] to Indoleamine 2, 3-dioxygenase
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y]
See all Indoleamine 2, 3-dioxygenase primary antibodies -
Description
Rabbit monoclonal [EPR1230Y] to Indoleamine 2, 3-dioxygenase -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Indoleamine 2, 3-dioxygenase aa 100-200.
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Positive control
- WB: Wild-type A549 Treated IFN gamma, SK-OV-3 and HeLa treated with 10ng/ml IFN-gamma for 24 hours whole cell lysate,
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1230Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
Target
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Function
Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. -
Pathway
Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2. -
Sequence similarities
Belongs to the indoleamine 2,3-dioxygenase family. - Information by UniProt
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Database links
- Entrez Gene: 3620 Human
- Omim: 147435 Human
- SwissProt: P14902 Human
- Unigene: 840 Human
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Alternative names
- 3-dioxygenase antibody
- I23O1_HUMAN antibody
- IDO 1 antibody
see all
Images
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Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)All lanes : Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157) at 1/2500 dilution
Lane 1 : Wild-type A549 Vehicle Control IFN gamma (0 ng/ml, 48 h) cell lysate
Lane 2 : Wild-type A549 Treated IFN gamma (25 ng/ml, 48 h) cell lysate
Lane 3 : IDO1 knockout A549 Vehicle Control IFN gamma (0 ng/ml, 48 h) cell lysate
Lane 4 : IDO1 knockout A549 Treated IFN gamma (25 ng/ml, 48 h) cell lysate
Lane 5 : SK-OV-3 cell lysate
Lane 6 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Lanes 1 - 6: Merged signal (red and green). Green - ab76157 observed at 40 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab76157 was shown to react with Indoleamine 2, 3-dioxygenase in treated wild-type A549 cells in Western blot with loss of signal observed in treated IDO1 knockout cell line ab266949 (IDO1 knockout cell lysate ab256948). Wild-type A549 and IDO1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab76157 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 2500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)](https://www.abcam.com/ps/products/76/ab76157/Images/ab76157-449571-anti-indoleamine-2-3-dioxygenase-antibody-epr1230y-westernblot-hela-human.jpg)
Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)Lane 1 : Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157) at 1/1000 dilution
Lane 2 : Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10ng/ml IFN-gamma for 24 hours whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 45 kDaThe expression of Indoleamine 2, 3-dioxygenase can be upregulated by IFN-gamma (PMID: 29685162). We are unsure how to define the extra bands.
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![Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)](https://www.abcam.com/ps/products/76/ab76157/Images/ab76157-3-benefits-of-recombinant-antibodies.png)
Anti-Indoleamine 2, 3-dioxygenase antibody [EPR1230Y] (ab76157)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab76157 has been referenced in 7 publications.
- McNulty DE et al. A High-Throughput Dose-Response Cellular Thermal Shift Assay for Rapid Screening of Drug Target Engagement in Living Cells, Exemplified Using SMYD3 and IDO1. SLAS Discov 23:34-46 (2018). PubMed: 28957646
- Wu X et al. Intrinsic Immunity Shapes Viral Resistance of Stem Cells. Cell 172:423-438.e25 (2018). PubMed: 29249360
- Xie Z et al. Immunotolerant indoleamine-2,3-dioxygenase is increased in condyloma acuminata. Br J Dermatol 177:809-817 (2017). PubMed: 28132413
- Takeshita K et al. Xenotransplantation of interferon-gamma-pretreated clumps of a human mesenchymal stem cell/extracellular matrix complex induces mouse calvarial bone regeneration. Stem Cell Res Ther 8:101 (2017). WB, ICC/IF ; Human . PubMed: 28446226
- Aldajani WA et al. Expression and regulation of immune-modulatory enzyme indoleamine 2,3-dioxygenase (IDO) by human airway epithelial cells and its effect on T cell activation. Oncotarget 7:57606-57617 (2016). PubMed: 27613847
- Yamazaki T et al. Effect of Ureaplasma parvum co-incubation on Chlamydia trachomatis maturation in human epithelial HeLa cells treated with interferon-?. J Infect Chemother 20:460-4 (2014). Human . PubMed: 24855914
- De Ravin SS et al. Tryptophan/kynurenine metabolism in human leukocytes is independent of superoxide and is fully maintained in chronic granulomatous disease. Blood 116:1755-60 (2010). PubMed: 20511543