Anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416)
Key features and details
- Mouse monoclonal [14C2] to Influenza A Virus M2 Protein
- Suitable for: ICC
- Reacts with: Influenza A
- Isotype: IgG1
Product nameAnti-Influenza A Virus M2 Protein antibody [14C2]
See all Influenza A Virus M2 Protein primary antibodies
DescriptionMouse monoclonal [14C2] to Influenza A Virus M2 Protein
Tested applicationsSuitable for: ICCmore details
Species reactivityReacts with: Influenza A
Full length protein corresponding to Influenza A Virus M2 Protein. Full length M2 protein from A/WSN/33-infected CV1 cell lysate.
EpitopeDetects the N-terminal of the Influenza A Virus M2 Protein.
- ICC/IF: Infected MDCK cells with Influenza A.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Concentration information loading...
PurityProtein G purified
Primary antibody notesInfluenza A virus is an enveloped virus encoding 10 polypeptides. RNA segment 7 encodes for two proteins: M1 (matrix 1) and M2 (matrix 2). M1 protein is encoded by an mRNA that is colinear, while M2 protein is synthesized from spliced mRNA. M2 protein is a transmembrane protein composed of three Domains: 1) 24 residues representing the N-terminal region, 2) 19 hydro-phobic residues that serve as a membrane anchor, and 3) 54 residues near the C-terminal in the cytoplasmic domain. The M2 protein has been found to play a role in Influenza replication and assembly of virion particles. Further experimentation has demonstrated that this protein is an acid-activated ion channel for virus replication.
Light chain typekappa
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
Use a concentration of 1 µg/ml.
RelevanceThe Matrix protein M2 forms a protons channel. When the environmental pH is lower than a threshold, the M2 channel is activated and selectively transports protons accross the membrane from the extracellular side to the cytoplasmic side. It is crucial for the uncoating process. When the virion is internalized into the endosome the channel can acidify the virion interior, promoting the dissociation of the viral matrix protein (M1) from the ribonucleoprotein (RNP) thus allowing the transport of the RNP from the virion into the cell’s nucleus. For some influenza virus subtypes, the M2 channel can elevate the intravesicular pH of the trans Golgi network, preventing the viral protein haemagglutinin, which is transported to the cell surface through the trans Golgi network, from incorrect maturation in an otherwise low pH environment.
Cellular localizationVirion membrane. Apical cell membrane; Single-pass type III membrane protein.
- Entrez Gene: 1461002 Influenza A
- Entrez Gene: 956528 Influenza A
- SwissProt: A0A2R3YRM7 Influenza A
- SwissProt: P06821 Influenza A
- SwissProt: Q67179 Influenza A
- Influenza A virus matrix protein M2 antibody
- M antibody
- M2 antibody
Datasheets and documents
ab5416 has been referenced in 62 publications.
- Pila-Castellanos I et al. Mitochondrial morphodynamics alteration induced by influenza virus infection as a new antiviral strategy. PLoS Pathog 17:e1009340 (2021). PubMed: 33596274
- Colant N et al. Escherichia Coli-Based Cell-Free Protein Synthesis for Iterative Design of Tandem-Core Virus-Like Particles. Vaccines (Basel) 9:N/A (2021). PubMed: 33669126
- Zhang W et al. Trojan Horse Delivery of 4,4'-Dimethoxychalcone for Parkinsonian Neuroprotection. Adv Sci (Weinh) 8:2004555 (2021). PubMed: 33977069
- Durgan J et al. Non-canonical autophagy drives alternative ATG8 conjugation to phosphatidylserine. Mol Cell 81:2031-2040.e8 (2021). PubMed: 33909989
- Blokhina EA et al. Plant-Produced Recombinant Influenza A Virus Candidate Vaccine Based on Flagellin Linked to Conservative Fragments of M2 Protein and Hemagglutintin. Plants (Basel) 9:N/A (2020). PubMed: 32013187