Anti-Influenza A Virus M2 Protein antibody [14C2] (ab5416)

Thank you very much for your patience.

Finally, I have a price quote and custom made confirmation from laboratory. We are happy to provide this product at lower cost than ab5416. The price would be £194 per vial or £582 for 3 vials of this product, excluding shipping charges which will be £16 for UK and 21 Euros for Germany.

Could you let me know if you want to go ahead with the order? I will then be happy to publish the datasheet of this custom made product.

Please note for proper quotation I would need the billing and shipping address.

I will look forward to hearing from you soon.

Thank you for your email.

I have sent a request to laboratory for quote; I will send you the details soon.

Many thanks for having patience!

Thank you for your email.

I just spoken to my colleague in laboratory; they have suggested that in order to justify the production cost for making it BSA and azide free at least 3 vials should be ordered. Could you let me know if you will buy 3 vials and then I will send you a quote.

I will look forward to hearing from you soon. Let me know if you have any questions and concerns.

Thank you for contacting us.

We can provide this product, BSA and azide free as a special request. Could you let me know how many vials you would like to buy?

Looking forward to hearing from you soon.

Thank you for contacting us.

Our Influenza A M2 Monoclonal Antibody (14C2) - product #ab5416 was shown to detect influenza A virus M2 strain A/PR/8/34 in the following reference:



Zebedee SL, Lamb RA"Influenza A virus M2 protein: monoclonal antibody restriction of virus growth and detection of M2 in virions."J Virol. 1988 Aug;62(8):2762-72.
(http://www.ncbi.nlm.nih.gov/pubmed/2455818)

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you foryour reply and forkindly providing the requested information.

I have investigated this with our source for the antibody and reviewed the details. It is worth consideringthat the Nandrop will be unable to distinguish between IgG and BSA. Also the OD280 nm values are very low and a little variable.It is difficult to draw an absolute conclusion but this does indicate that the antibody did not bind to the column. However, this can be confirmed with further investigation.

Unfortunately, experience has shown that the measurement of antibody recovery using the nanodrop is not always very accurate.We would therefore recommendto confirmrecovery of the antibody by an alternative method. For example, a 1D non reducing gel would provide an indication of the amount of antibody and its quality. There shouldbe asingle antibody band of approximately 160 kDa. The intensity of this band will be related to the amount of antibody recovered.

If the recovery is in fact found to be low then it means that for some reason the antibody has failed to bind to the resin. The antibody will therefore be in the flow through. Again a 1D non reducing gel of the flow through sample could confirm this (the material will have two bands IgG and BSA). In this case you could repeat the binding to the resin after adding a second volume of binding buffer. The second addition of binding buffer will make sure that the pH of the antibody is definitely correct (pH 8.0) and that the ionic strength of the antibody buffer is sufficiently raised to promote binding. The kits are provided with sufficient excess buffers to enablethis procedure. The addition of more binding buffer has proved very successful in the past when dealing with troublesome mouse antibodies.

I hope this will be helpful to you. Please dolet me know how you get on. If you still have some concerns, please do not hesitate to contact me with further information and details of how you would like to proceed.

Thank you for contacting Abcam regarding this,
I have had the chance to speak with the lab about your question. We have not tested whether this antibody can be used as a neutralizing antibody. The authors in the following paper used this antibody in a blocking assay. They found out that "The level of M2 surface expression in A/Udorn virus-infected MDCK cells was found to be reduced to ∼60% of control levels in cells incubated with the 14C2 antibody. In contrast, M2 surface expression levels in A/WSN virus-infected cells were decreased by only ∼5-15%, and A/WSN virus assembly appeared to be unaffected by the M2 antibody treatment." It appears that the blocking is also virus strain dependent.

http://www.sciencedirect.com/science/article/pii/S0042682285714985

I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

I would suggest to prepare buffers that are recommended in the following Western Blotting protocol : https://www.abcam.com/index.html?pageconfig=resource&rid=13045 .

Thank you very much for your inquiry. I have contacted the laboratory for information on the ab5416. However the laboratory has not tested this antibody neither on your strain. I did a sequence alignement of the immunogen and your sequence. Please see the results below. Indeed, the alignement is very good. However as this is a monoclonal antibody, we do not know the epitope and can therefore not guarantee crossreactivity. http://www.ebi.ac.uk/Tools/services/web_clustalw2/toolresult.ebi?tool=clustalw2&jobId=clustalw2-I20111223-121219-0395-63524695-pg We do have two other antibodies against the Influenza A Virus M2 protein, the ab56086 and the ab94925. I have already done the alignement of the immunogen of ab56086 with your protein of interest. The overlapp is 100%. I would therefore expect that this antibody does recognize your protein as well. xxx I am checking with the laboratory for the alingment of ab94925 with your protein (http://www.uniprot.org/uniprot/A5GX42) and will get back to you. If the customer would like to test the ab56086 on the Jersey Strain, I would be pleased to provide also a testing discount (www.abcam.com/collaborationdiscount). Please let me know if you would like to know more about this testing discount. Please do not hesitate to contact me, should you or the customer have any other question or concern.

Thank you for your enquiry. This product was created against whole M2 protein purified from A/WSN/33-infected CV1 cell lysate. Unfortunately, the exact epitope for this polyclonal antibody is not known at this time. The antibody was not tested against all subtypes, but the M2 protein should be identical in the influenza A virus subtypes.