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Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)

Reviews (2)Q&A (5)References (91)
Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)
  • Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)

Key features and details

  • Mouse monoclonal [AA5H] to Influenza A Virus Nucleoprotein
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Influenza A
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Influenza A Virus Nucleoprotein antibody [AA5H]
    See all Influenza A Virus Nucleoprotein primary antibodies

  • Description

    Mouse monoclonal [AA5H] to Influenza A Virus Nucleoprotein

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Influenza A

  • Immunogen

    Tissue, cells or virus corresponding to Influenza A Influenza A Virus Nucleoprotein. Influenza A/Puerto Rico/8/34 (H1N1) and A/Bangkok/1/79 (H3N2) viruses.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    >95% pure (SDS-PAGE).

  • Clonality

    Monoclonal

  • Clone number

    AA5H

  • Myeloma

    P3x63-Ag8.653

  • Isotype

    IgG2a

  • Light chain type

    unknown

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab20343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
IHC-P
1/1000.
Notes
ICC/IF
Use at an assay dependent concentration.
IHC-P
1/1000.

Target

  • Relevance

    The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).

  • Cellular localization

    Host cell nucleus

  • Database links

    • Alternative names

      • Common flu NP antibody
      • Influenza A virus NP antibody
      • NP antibody
      • Nucleocapsid protein antibody
      • Nucleoprotein antibody
      • Protein N antibody
      • Seasonal Influenza A (H1N1) Nucleocapsid Protein antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)
      Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)

      Ab20343 positively staining formaldehyde fixed Influenza A infected human 293T cells (red) counterstained with AUF-1 (green). ab20343 was used at 1/1000 with no antigen retrieval.

      This image is courtesy of an Abreview submitted by Paul Digard on 24 August 2005. We do not have any further information relating to this image.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)Image from Dr RK Nelli at al, BMC Vet Res. 2010 Jan 27;6:4, Fig 5.
      ab20343 staining Influenza A Virus Nucleoprotein in porcine lung tissue by Immunohistochemistry (Formalin-fixed, paraffin-embedded sections).
      Host receptor binding assays with H1N1 classical swine strain (A/Sw/Iowa/15/30), a subtype closely related to the human 1918 pandemic influenza virus. Briefly, paraffin embedded 5 µm sections of lung tissues were deparaffinised in xylene and rehydrated by alcohol. Deparaffinised tissue sections were incubated with TPCK trypsin treated swine influenza virus for 24 hours at 37°C. Paradoxically, we found that mammalian H1N1 virus binds more efficiently at 37°C than at the usual 4°C. The sections were washed, blocked with goat serum for 30 minutes, and incubated with ab20343 at a 1/1000 dilution, overnight in a humidified chamber at 4°C. A secondary antibody, FITC-labelled goat anti-mouse IgG was applied at 1/500 dilution for 2 hours at room temperature. After three further washes with TBS, the sections were mounted with ProLong Gold anti-fa
    • Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)
      Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343)Image is courtesy of an AbReview submitted by Dr Maria Amorim.

      Immunocytochemical immunoflurescence analysis of Formaldehyde-fixed human kidney epithelial cells, labelling Influenza A Virus M1 matrix protein with ab20910 at a dilution of 1/500 incubated fro 1 hour at 18°C in 1% FCS PBS. Blocking was with 1% serum incubated for 30 minutes at 18°C. Secondary was a donkey anti-goat Alexa Fluor® 568 undiluted. Cells were transfected with either GFP-M1 or pCDNA2-NP, as indicated on the left hand side of the figure. 24h later cells were fixed and stained for M1 using ab20910 (red) or NP using ab20343 (grey). The abcam 20910 detected M1 in cells transfected with GFP-M1 but not pCDNA3-NP. Moreover, the level of co-localization between GFP-M1 and ab20910 was quite good.

      See Abreview

    References (91)

    Publishing research using ab20343? Please let us know so that we can cite the reference in this datasheet.

    ab20343 has been referenced in 91 publications.

    • Liu H  et al. Antiviral Effects of ABMA and DABMA against Influenza Virus In Vitro and In Vivo via Regulating the Endolysosomal Pathway and Autophagy. Int J Mol Sci 23:N/A (2022). PubMed: 35409297
    • Francese R  et al. Identification of a βCD-Based Hyper-Branched Negatively Charged Polymer as HSV-2 and RSV Inhibitor. Int J Mol Sci 23:N/A (2022). PubMed: 35955832
    • Li W  et al. XiaoEr LianHuaQinqGan alleviates viral pneumonia in mice infected by influenza A and respiratory syncytial viruses. Pharm Biol 60:2355-2366 (2022). PubMed: 36444944
    • Humphries F  et al. A diamidobenzimidazole STING agonist protects against SARS-CoV-2 infection. Sci Immunol 6:N/A (2021). PubMed: 34010139
    • Melano I  et al. Effects of Basic Amino Acids and Their Derivatives on SARS-CoV-2 and Influenza-A Virus Infection. Viruses 13:N/A (2021). PubMed: 34372507
    View all Publications for this product

    Customer reviews and Q&As

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    Submit a question

    1-5 of 5 Q&A

    Question

    I am trying to do immunoprecipitation with one of your influenza virus antibodies. Anti-Influenza A Virus Nucleoprotein antibody [AA5H] (ab20343) I have read your online protocol for immunoprecipitation https://www.abcam.com/ps/pdf/protocols/Immunoprecipitation%20protocol%20(IP).pdf and I have a doubt, at the point immunoprecipitation: Point 7. Finally, remove the last supernatant and add 25-50 μl of 2x loading buffer. Boil at 95-100°C for 5 minutes to denature the protein and separate it from the protein-A/G beads, then centrifuge and keep the supernatant where the protein is now. Once I do the elution in 2x loading buffer, do I need to further dilute the sample with distilled water 1 in 2 before loading the sample into the gel? Otherwise I will be loading the samples in 2x loading buffer..... Thank you very much for your help in advance!

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    Answer

    You will not need to dilute your sample any further after eluting from the beads. As the protocol says, you can after boiling load your sample directly onto your gel, without it affecting anything. If there is anything else I can help you with, please let me know.

    Read More

    Question

    I’ve been using the influenza A nucleoprotein antibody [AA5H] (ab20343) to stain for influenza A PR/8 (H1N1) infected cells. It’s worked great so far. However, I haven’t had success in staining cells (MDCK) with influenza A X-31 (H3N2). According to the specs, AA5H should work with H3N2 subtype. Was wondering if abCam has any information or insight on using AA5H for the X31 strain. I’ve also tried primary antibody 431-FITC on X31; however, I have not had success with that either.

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    Answer

    Thanks for your inquiry. The monoclonal antibody to Influenza A Nucleoprotein, catalog ab20343, has been tested and is reactive with the following Influenza A strains: H1N1 A/Puerto Rico/8/34A/USSR/90/77 A/England/333/80 A/Chile/1/83 A/Dunedin/6/83 A/Victoria/7/83 H2N2 A/Ann Arbor/6/60 H3N2 A/Aichi/2/68 A/Port Chambers/1/73 A/Bangkok/2/79 A/Korea/1/82 A/Philippines/2/82 A/Caen/1/84 A/Pennsylvania/27/84 For ab81126 : we used a vaccine as the immunogen of ab81126, and used the recombinant protein NP to screen positive clones. So the antibody of ab81126 can be used to detect the NP protein. We are not sure whether this antibody can react with all strains of influenza A virus, because we haven't done the relating tests. I hope this was helpful. Please feel free to contact me if you require further assistance.

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    Question

    Can this antibody detect intact influenza A virus?

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    Answer

    Thank you for your inquiry. The laboratory confirmed that they have not tested ab20343 against the intact virus.  The following link is to a published reference that describes the development and original characterizaton of this clone.  The paper includes IFA testing of cells infected with viruses reisolated from clinical Influenza A specimens. http://jcm.asm.org/cgi/reprint/23/2/240 Catalog ab20343, Clone AA5H, is described as Clone A1 in the paper.  I hope this information helps. Please contact me with any other questions.

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    Question

    My post doc would like to know if you have available the specific recognition sequence for this antibody.

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    Answer

    Thank you for your patience in this matter. I have received a reply from the originator of ab20343 (Influenza A Virus Nucleoprotein antibody [AA5H]). All the characterization information we have on this product is from the following published reference: http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=268620&blobtype=pdf Note that Clone AA5H is Clone A1 in the paper. I hope this information helps. Please don't hesitate to contact us again if you have further questions.

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    Question

    Your company offers a monoclonal antibody against influenza A-Nucleoprotein. It is offered for the application in immunocytochemistry. My question is: Is it possible to use this antibody in immunohistochemistry in formalin fixed and paraffin embedded tissue? Or only in cryostat sections?

    Read More
    Answer

    Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in either formalin-fixed paraffin-embedded or frozen sections. It has been tested in immunocytochemistry and immunofluorescence in cultured cells. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image). I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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