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Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)

Submit a review Q&A (5)References (33)
Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)
  • Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)
  • Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)
  • Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)

Key features and details

  • Mouse monoclonal [C43] to Influenza A Virus Nucleoprotein
  • Suitable for: WB, ELISA, ICC/IF, Flow Cyt
  • Reacts with: Influenza A
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

HRP

Overview

  • Product name

    Anti-Influenza A Virus Nucleoprotein antibody [C43]
    See all Influenza A Virus Nucleoprotein primary antibodies

  • Description

    Mouse monoclonal [C43] to Influenza A Virus Nucleoprotein

  • Host species

    Mouse

  • Specificity

    Reacts with NP of all influenza A viruses so far tested, including seasonal H2N2, H3N2(A/Sydney/5/1997), and H5N1(A/crow/Kyoto53/2004), H5N1 (A/duck/Egypt/D2br10/07), H5N1(A/duck/HK/342/78), H5N2(A/crow/Kyoto/53/04), H9N1, H9N2 (A/Turkey/Wisconsin/1/66) and H1N1 (seasonal: A/New Caledonia/20/99. Pandemic: A/Suita/01/2009 and swine: A/PuertoRico/8/34).

    No cross reactivity with influenza B viruses.

  • Tested applications

    Suitable for: WB, ELISA, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Influenza A

  • Immunogen

    Tissue, cells or virus corresponding to Influenza A Virus Nucleoprotein. Human Influenza A Virus H3N2 strain
    Database link: P69291

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 6
    Constituents: 50% Glycerol, 49% PBS
  • Concentration information loading...

  • Purity

    Proprietary Purification

  • Purification notes

    Produced in serum-free medium and purified by proprietary chromatography procedure under mild conditions. 90~95% pure by SDS-PAGE

  • Clonality

    Monoclonal

  • Clone number

    C43

  • Isotype

    IgG2a

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab128193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/300 - 1/1000.
ELISA
Use at an assay dependent concentration.
ICC/IF
1/200.
Flow Cyt
Use at an assay dependent concentration. PubMed: 24067955

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Notes
WB
1/300 - 1/1000.
ELISA
Use at an assay dependent concentration.
ICC/IF
1/200.
Flow Cyt
Use at an assay dependent concentration. PubMed: 24067955

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • Relevance

    The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).

  • Cellular localization

    Host cell nucleus

  • Database links

    • Alternative names

      • Common flu NP antibody
      • Influenza A virus NP antibody
      • NP antibody
      • Nucleocapsid protein antibody
      • Nucleoprotein antibody
      • Protein N antibody
      • Seasonal Influenza A (H1N1) Nucleocapsid Protein antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)
      Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)
      Immunofluorescence assay of MDCK cells derived from canine kidney cells, and A549 cells derived from human lung carcinoma cells, that were infected with H1N1 influenza virus (A/PuertoRico/8/34). Samples were taken at 3, 9, and 24 hours post-infection. Influenza A Virus Nucleoprotein antibody (ab128193) efficiently detected virus-infected MDCK and A549 cells as early as 3 h after infection. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an green flurescent secondary antibody.
    • Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)
      Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)
      Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) at 1/1000 dilution + MDCK cells infected with Influenza A virus (H1N1) PuertoRico/8/34

      Secondary
      Rabbit Anti-Mouse IgG H&L (HRP) (ab97046) at 1/10000 dilution
    • Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)
      Immunocytochemistry/ Immunofluorescence - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)
      Immunofluorescence assay of 293T cells expressing HA or NP of pandemic (H1N1) 2009 influenza A virus (A/Suita/1/2009). Influenza A Virus Nucleoprotein antibody (ab128193) specifically recognized NP-expressing cells while a proprietary mouse anti-HA monoclonal antibody specifically recognized HA.
    • Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)
      Western blot - Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193)
      All lanes : Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) at 1/300 dilution

      Lanes 1 & 6 & 11 : MDCK cells mock infected
      Lane 2 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 3 hrs post-infection
      Lane 3 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 9 hrs post-infection
      Lane 4 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 24 hrs post-infection
      Lane 5 : MDCK cells infected with H1N1 (A/PuertoRico/8/34) collected 48 hrs post-infection
      Lane 7 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 3 hrs post-infection
      Lane 8 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 9 hrs post-infection
      Lane 9 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 24 hrs post-infection
      Lane 10 : MDCK cells infected with H5N2 (A/crow/Kyoto/53/04) collected 48 hrs post-infection
      Lane 12 : MDCK cells infected H5N1
      (A/duck/HK/342/78)collected 3 hrs post-infection
      Lane 13 : MDCK cells infected with H5N1
      (A/duck/HK/342/78) collected 9 hrs post-infection
      Lane 14 : MDCK cells infected with H5N1
      (A/duck/HK/342/78) collected 24 hrs post-infection
      Lane 15 : MDCK cells infected with H5N1
      (A/duck/HK/342/78) collected 48 hrs post-infection


      Tubulin was used as loading control.

    References (33)

    Publishing research using ab128193? Please let us know so that we can cite the reference in this datasheet.

    ab128193 has been referenced in 33 publications.

    • Liu HY  et al. Single-virus tracking with quantum dots in live cells. Nat Protoc 18:458-489 (2023). PubMed: 36451053
    • Wu W  et al. MicroRNA let-7 Suppresses Influenza A Virus Infection by Targeting RPS16 and Enhancing Type I Interferon Response. Front Cell Infect Microbiol 12:904775 (2022). PubMed: 35873150
    • John SP  et al. Small-molecule screening identifies Syk kinase inhibition and rutaecarpine as modulators of macrophage training and SARS-CoV-2 infection. Cell Rep 41:111441 (2022). PubMed: 36179680
    • Kelly JN  et al. Comprehensive single cell analysis of pandemic influenza A virus infection in the human airways uncovers cell-type specific host transcriptional signatures relevant for disease progression and pathogenesis. Front Immunol 13:978824 (2022). PubMed: 36268025
    • Pila-Castellanos I  et al. Mitochondrial morphodynamics alteration induced by influenza virus infection as a new antiviral strategy. PLoS Pathog 17:e1009340 (2021). PubMed: 33596274
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Question

    Inquiry: I would like to know the peptide sequence to which this antibody binds to in the Influenza A nucleoprotein.

    Read More
    Answer

    The immunogen of this antibody is human Influenza A Virus (H2N2) Okada strain.

    Unfortunately, we have not determined the epitope recognized by this antibody.

    Read More

    Question

    Thank you for response Abcam Order # was 1206990.

    Best Regards.

    Read More
    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1230375.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Question

    Thank you for your reply on this complaint.

    Please see below response from customer on your additional queries-

    Customer's Reply -

    I am surprised to know the information provided by us still not sufficient to troubleshoot.
    1. By the way the chemicon antibody is only for IFA applications. If chemicon has antibody with western blot and immunoprecipitation applications, perhaps I wouldn't be getting abcam antibody.

    2. the construct contains full NP sequence without any tag.

    3. We use H1N1, H3 N2 strains, in addition I have several seasonal isolated. all the strains been confirmed. But for this assay we used H1N1 and H3N2 strains.

    4. For your kind information in my lab every student does western blot with same detection system. We do not have any problem with detection method.

    5. I do not have purified NP protein. If I have purified protein probably I would have tried to raise antibody in the lab itself.

    Hope this information is sufficient.

    Kindly check and advise urgently as this customer is not comfortable providing additional information.

    Read More
    Answer

    Thank you for your response to my further questions. It is very much appreciated.

    When experiments are carried out using transfected cells, it is very important to provide information about the construct and any positive controls used to make sure that the experiments are nicely controlled. Without these details and the exact strains of the virus, it is hard to troubleshoot and to provide sufficient technical support.

    We have not received any similar complaint about ab128193, thus not aware of any QC issues. Currently, we have the same batch in stock your customer has tried. I could offer you a new vial of ab128193 or one vial of any alternative antibodies against the same target protein (Influenza A Virus Nucleoprotein).

    We have the following antibodies which are suitable for Western blot application:

    - https://www.abcam.com/ab43821: Anti-Influenza A Virus Nucleoprotein antibody [9G8]

    - https://www.abcam.com/ab66191: Anti-Influenza A Virus Nucleoprotein antibody [B247M]

    - https://www.abcam.com/ab81126: Anti-Influenza A Virus Nucleoprotein antibody [15]

    Please could you discuss it with your customer and let me know how he/she would like to proceed.

    I look forward to hearing from you soon.

    Read More

    Question

    Dear Sir：



    We are interested in ab128193,



    Please help us confirm which epitope are recognize by ab128193？



    Best wishes ,



    Wang, Jianfei(王剑飞)

    Product Support Specialist(产品支持专家)

    -----------



    艾美捷科技有限公司 Amyjet Sci, Inc.

    电话/Phone：+86-027-84454412 +86-027-59846291
    传真/Fax：+86-027-84454876
    网址/Website：http://www.amyjet.com/
    生物实验室产品解决方案供应商，为您提供最合适的实验解决方案
    众多国际知名品牌中国区域代理，为您提供最有保障的产品和服务

    Read More
    Answer

    Thank you for contacting us.

    The immunogen of ab128193 is whole virus of Human Influenza AVirus (H2N2) OKada strain. We, however, have not mapped the epitope of this antibody.

    I am sorry for not being able to provide you any useful information this time. Please do not hesitate to contact us if you need any more advice or information in other aspects.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Dear All,
    I ordered the Anti-Influenza A Virus Nucleoprotein antibody [C43] (ab128193) as you stated (https://www.abcam.com/Influenza-A-Virus-Nucleoprotein-antibody-C43-ab128193.html) that it would have reacted with NP of all influenza A subtypes and the Mouse monoclonal [B247M] to influenza A virus Nucleoprotein (ab66191). I have been testing both of them in Western Blot analysis and none of them worked with the H7N3 avian influenza subtype that I am currently using for my studies. Therefore I had to use another Mab (AbCam) from a different lab. I would ask you to give me you best quotation for this Mab: “ Mouse monoclonal [9G8] to influenza A virus Nucleoprotein (ab43821)”.
    Looking forward to hear from you soon
    Best wishes,

    Read More
    Answer

    Thank you for contacting us.

    I am sorry if ab128193 did not work as you expected.

    H7N3 subtype of avian Influenza A virus has not been tested with this antibody. I would recommend you to test IF with the virus-infected cells or ELISA with inactivated purified antigen of N7H3 to examine the reactivity of C43 antibody with N7H3 subtype.

    In regards to ab43821, it should recognize H7N3 influenza; however, it was not tested on this viral strain.

    You may have received the quotation in a separate email.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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