Recombinant Anti-Influenza A virus Nucleoprotein antibody [HL1089] (ab308393)
![Western blot - Anti-Influenza A virus Nucleoprotein antibody [HL1089] (ab308393)](https://www.abcam.com/ps/products/308/ab308393/Images/ab308393-17-AntiInfluenza-A-virus-Nucleoprotein-antibody-HL1089-WB-HEK293T-transfected-cells.jpg "Western blot - Anti-Influenza A virus Nucleoprotein antibody [HL1089] (ab308393)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [HL1089] to Influenza A virus Nucleoprotein - BSA and Azide free
- Suitable for: ICC/IF, WB, IHC-P, Indirect ELISA
- Reacts with: Influenza A
Overview
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Product name
Anti-Influenza A virus Nucleoprotein antibody [HL1089]
See all Influenza A virus Nucleoprotein primary antibodies -
Description
Rabbit monoclonal [HL1089] to Influenza A virus Nucleoprotein - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, Indirect ELISAmore details -
Species reactivity
Reacts with: Influenza A -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Influenza A viral lysates, transfected HEK293T cell lysate IHC-P: flu A_NP (Kansas/2017/H3N2) transfected 293T cell FFPE Cell Pellet Block, Indirect ELISA: recombinant influenza A virus nucleoprotein (A/Kansas/2017/H3N2),influenza A viral lysate, influenza B viral lysate, recombinant full-length Influenza A virus NP ICC/IF: Transfected HEK293T cells, Influenza A Virus infected cells
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General notes
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HL1089 -
Isotype
IgG
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab308393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100 - 1/2000.
|
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| WB |
1/1000 - 1/5000.
|
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| IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| Indirect ELISA |
Use a concentration of 1 µg/ml.
|
| Notes |
|---|
|
ICC/IF
1/100 - 1/2000. |
|
WB
1/1000 - 1/5000. |
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
Indirect ELISA
Use a concentration of 1 µg/ml. |
Target
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Relevance
Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus. -
Database links
- SwissProt: P15682 Influenza A
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Alternative names
- Nucleocapsid protein antibody
- Protein N antibody
Images
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Western blot - Anti-Influenza A virus Nucleoprotein antibody [HL1089] (ab308393)All lanes : Anti-Influenza A virus Nucleoprotein antibody [HL1089] (ab308393) at 1/5000 dilution
Lane 1 : Non-transfected (–) HEK293T cell lysate
Lane 2 : DDDDK-tag tagged flu A_NP (California/2009/H1N1) transfected (+) HEK293T cell lysate
Lane 3 : DDDDK-tag tagged flu A_NP (A/Kansas/2017/H3N2) transfected (+) HEK293T cell lysate
Lane 4 : DDDDK-tag tagged flu A_NP (A/Astrakhan/3212/2020/H5N8) transfected (+) HEK293T cell lysate
Lane 5 : DDDDK-tag tagged flu A_NP (A/Jiangsu/428/2021/H10N3) transfected (+) HEK293T cell lysate
Lane 6 : DDDDK-tag tagged flu A_NP (B/Victoria/02/1987) transfected (+) HEK293T cell lysate
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody -
![Western blot - Anti-Influenza A virus Nucleoprotein antibody [HL1089] (ab308393)](https://www.abcam.com/ps/products/308/ab308393/Images/ab308393-16-AntiInfluenza-A-virus-Nucleoprotein-antibody-HL1089-WB-HEK293T-human.jpg)
Western blot - Anti-Influenza A virus Nucleoprotein antibody [HL1089] (ab308393)All lanes : Anti-Influenza A virus Nucleoprotein antibody [HL1089] (ab308393) at 1/5000 dilution
Lane 1 : Non-transfected (–) HEK293T cell lysate
Lane 2 : DDDDK-tag tagged flu A_NP (A/Kansas/2017/H3N2) transfected (+) HEK293T cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody -
![Western blot - Anti-Influenza A virus Nucleoprotein antibody [HL1089] (ab308393)](https://www.abcam.com/ps/products/308/ab308393/Images/ab308393-15-AntiInfluenza-A-virus-Nucleoprotein-antibody-HL1089-WB--viral-lysates.jpg)
Western blot - Anti-Influenza A virus Nucleoprotein antibody [HL1089] (ab308393)All lanes : Anti-Influenza A virus Nucleoprotein antibody [HL1089] (ab308393) at 1/1000 dilution
Lane 1 : Influenza A viral lysate (A/Brisbane/59/07 (H1N1))
Lane 2 : Influenza A viral lysate (A/New Cal/20/99 (H1N1))
Lane 3 : Influenza A viral lysate (A/Puerto Rico/8/34 (H1N1))
Lane 4 : Influenza A viral lysate (A/Singapore/63/04 (H1N1))
Lane 5 : Influenza A viral lysate (A/Taiwan/42/06 (H1N1))
Lane 6 : Influenza A viral lysate (A/California/07/09 (H1N1))
Lane 7 : Influenza A viral lysate (A/Canada/6294/09 (H1N1))
Lane 8 : Influenza A viral lysate (A/Mexico/4108/09 (H1N1))
Lane 9 : Influenza A viral lysate (A/New York/01/09 (H1N1))
Lane 10 : Influenza A viral lysate (A/New York/02/09 (H1N1))
Lane 11 : Influenza A viral lysate (A/New York/03/09 (H1N1))
Lane 12 : Influenza A viral lysate (A/Brisbane/10/07 (H3N2))
Lane 13 : Influenza A viral lysate (A/Hong Kong/8/68 (H3N2))
Lane 14 : Influenza A viral lysate (A/Perth/16/09 (H3N2))
Lane 15 : Influenza A viral lysate (A/Texas/50/12 (H3N2))
Lane 16 : Influenza A viral lysate (A/Wisconsin/67/05 (H3N2))
Lysates/proteins at 1 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody -
![Indirect ELISA - Anti-Influenza A virus Nucleoprotein antibody [HL1089] (ab308393)](https://www.abcam.com/ps/products/308/ab308393/Images/ab308393-14-AntiInfluenza-A-virus-Nucleoprotein-antibody-HL1089-indirect-ELISA--viral-lysates.jpg)
Indirect ELISA - Anti-Influenza A virus Nucleoprotein antibody [HL1089] (ab308393)Indirect ELISA analysis performed by coating plate with viral lysate derived from different strains of Influenza A virus (i.e., A/Brisbane/59/07 (H1N1); A/New Cal/20/99 (H1N1); A/Puerto Rico/8/34 (H1N1); A/Taiwan/42/06 (H1N1); A/California/07/09 (H1N1); A/Mexico/4108/09 (H1N1); A/New York/01/09 (H1N1); A/New York/02/09 (H1N1); A/New York/03/09 (H1N1); A/Canada/6294/09 (H1N1); A/Singapore/63/04 (H1N1); A/Brisbane/10/07 (H3N2); A/Perth/16/09 (H3N2); A/Texas/50/12 (H3N2); A/Wisconsin/67/05 (H3N2); A/Hong Kong/8/68 (H3N2)) and Influenza B virus (i.e., B/Florida/02/06) (1-0.2 μg). Coated protein was probed with Influenza A virus NP (nucleoprotein) antibody [HL1089] (ab308393) (1 μg/mL). Rabbit IgG antibody (HRP) (1/10000) was used to detect bound primary antibody.
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![Indirect ELISA - Anti-Influenza A virus Nucleoprotein antibody [HL1089] (ab308393)](https://www.abcam.com/ps/products/308/ab308393/Images/ab308393-13-AntiInfluenza-A-virus-Nucleoprotein-antibody-HL1089-indirect-ELISA--purified-full-length-protein.jpg)
Indirect ELISA - Anti-Influenza A virus Nucleoprotein antibody [HL1089] (ab308393)Indirect ELISA analysis performed by coating plate with recombinant full-length Influenza A virus NP (nucleoprotein) antibody (ab308393) (50 ng). Coated protein was probed with Influenza A virus Nucleoprotein antibody [HL1089] (20000-4.77*10-3 pM). Rabbit IgG antibody (HRP) (1/10000) was used to detect bound primary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A virus Nucleoprotein antibody [HL1089] (ab308393)](https://www.abcam.com/ps/products/308/ab308393/Images/ab308393-12-AntiInfluenza-A-virus-Nucleoprotein-antibody-HL1089-IHCP-HEK293T-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Influenza A virus Nucleoprotein antibody [HL1089] (ab308393)Immunohistochemical analysis of flu A_NP (Kansas/2017/H3N2) transfected HEK293T cell FFPE Cell Pellet Block labeling Influenza A virus Nucleoprotein (green) using ab308393 at 1/1000 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min
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![Immunocytochemistry/ Immunofluorescence - Anti-Influenza A virus Nucleoprotein antibody [HL1089] (ab308393)](https://www.abcam.com/ps/products/308/ab308393/Images/ab308393-3-AntiInfluenza-A-virus-Nucleoprotein-antibody-HL1089-ICC-HEK293T-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Influenza A virus Nucleoprotein antibody [HL1089] (ab308393)For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of Influenza A virus Nucleoprotein using ab308393 at 1/2000 dilution (green). -
![Immunocytochemistry/ Immunofluorescence - Anti-Influenza A virus Nucleoprotein antibody [HL1089] (ab308393)](https://www.abcam.com/ps/products/308/ab308393/Images/ab308393-2-AntiInfluenza-A-virus-Nucleoprotein-antibody-HL1089-ICC--TI.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Influenza A virus Nucleoprotein antibody [HL1089] (ab308393)For immunofluorescence analysis, Influenza A and B Virus infected cells slide and permeabilized for detection of Influenza A virus Nucleoprotein using ab308393 at 1/100 dilution (green). -
![Anti-Influenza A virus Nucleoprotein antibody [HL1089] (ab308393)](https://www.abcam.com/ps/products/308/ab308393/Images/ab308393-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-Influenza A virus Nucleoprotein antibody [HL1089] (ab308393)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab308393 has not yet been referenced specifically in any publications.
