Recombinant Anti-Inhibin beta A antibody [EPR2785(2)] (ab128958)
![OI-RD Scanning - Anti-Inhibin beta A antibody [EPR2785(2)] (ab128958)](https://www.abcam.com/ps/products/128/ab128958/Images/ab128958-142858-anti-inhibin-beta-a-antibody-epr27852.jpg "OI-RD Scanning - Anti-Inhibin beta A antibody [EPR2785(2)] (ab128958)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2785(2)] to Inhibin beta A
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Inhibin beta A antibody [EPR2785(2)]
See all Inhibin beta A primary antibodies -
Description
Rabbit monoclonal [EPR2785(2)] to Inhibin beta A -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
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Positive control
- Recombinant Human Inhibin beta A protein
Fetal brain, fetal liver and mouse brain lysates
- Recombinant Human Inhibin beta A protein
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.93 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2785(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 47 kDa).
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 47 kDa). |
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
Target
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Function
Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3624 Human
- Entrez Gene: 16323 Mouse
- Entrez Gene: 29200 Rat
- Omim: 147290 Human
- SwissProt: P08476 Human
- SwissProt: Q04998 Mouse
- SwissProt: P18331 Rat
- Unigene: 583348 Human
see all -
Alternative names
- Activin beta-A chain antibody
- EDF antibody
- Erythroid differentiation factor antibody
see all
Images
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OI-RD Scanning - Anti-Inhibin beta A antibody [EPR2785(2)] (ab128958)
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![Western blot - Anti-Inhibin beta A antibody [EPR2785(2)] (ab128958)](https://www.abcam.com/ps/products/128/ab128958/Images/ab128958-142860-anti-inhibin-beta-a-antibody-epr27852-western-blot.jpg)
Western blot - Anti-Inhibin beta A antibody [EPR2785(2)] (ab128958)Anti-Inhibin beta A antibody [EPR2785(2)] (ab128958) at 1/1000 dilution + Human Inhibin beta A recombinant protein at 0.001 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 47 kDa -
![Western blot - Anti-Inhibin beta A antibody [EPR2785(2)] (ab128958)](https://www.abcam.com/ps/products/128/ab128958/Images/ab128958-142859-anti-inhibin-beta-a-antibody-epr27852-western-blot.jpg)
Western blot - Anti-Inhibin beta A antibody [EPR2785(2)] (ab128958)All lanes : Anti-Inhibin beta A antibody [EPR2785(2)] (ab128958) at 1/1000 dilution
Lane 1 : Fetal brain lysate
Lane 2 : Fetal liver lysate
Lane 3 : Mouse brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 47 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted? -
![Anti-Inhibin beta A antibody [EPR2785(2)] (ab128958)](https://www.abcam.com/ps/products/128/ab128958/Images/ab128958-4-benefits-of-recombinant-antibodies.png)
Anti-Inhibin beta A antibody [EPR2785(2)] (ab128958)
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab128958 has been referenced in 2 publications.
- Xian S et al. Activation of activin/Smad2 and 3 signaling pathway and the potential involvement of endothelial-mesenchymal transition in the valvular damage due to rheumatic heart disease. Mol Med Rep 23:N/A (2021). PubMed: 33179113
- Wu Y et al. Autocrine transforming growth factor-ß/activin A-Smad signaling induces hepatic progenitor cells undergoing partial epithelial-mesenchymal transition states. Biochimie 148:87-98 (2018). PubMed: 29544731