Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)

Submit a review Submit a question References (14)
Western blot - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)
  • Western blot - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)
  • Western blot - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)
  • Indirect ELISA - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)
  • Western blot - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)
  • Immunoprecipitation - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)
  • Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)
  • Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)
  • Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16635] to iNOS - BSA and Azide free
  • Suitable for: ICC/IF, Indirect ELISA, WB, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 647 Carrier Free Unconjugated

Overview

  • Product name

    Anti-iNOS antibody [EPR16635] - BSA and Azide free
    See all iNOS primary antibodies

  • Description

    Rabbit monoclonal [EPR16635] to iNOS - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Indirect ELISA, WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: RAW 264.7 treated with 0.1 µg/mL LPS for 6 hours, HepG2 treated with 10 µg/mL LPS for 6 hours,whole cell lysates; Human fetal brain lysate; L6 treated with 50 ng/ml IL-1 beta, 20 ng/ml TNF-alpha and 100U/ml IFN-gamma for 24 h, whole cell lysate; ICC/IF: RAW 264.7 cells treated with LPS (0.1 µg/mL), for 6 hours. IP: RAW 264.7 whole cell lysate treated with 1µg/mL LPS for 24h.

  • General notes

    ab213987 is the carrier-free version of ab178945.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab213987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/500.
Indirect ELISA
Use at an assay dependent concentration.
WB
1/1000. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa).
IP
1/100.
Notes
ICC/IF
1/500.
Indirect ELISA
Use at an assay dependent concentration.
WB
1/1000. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa).
IP
1/100.

Target

  • Function

    Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.

  • Tissue specificity

    Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.

  • Sequence similarities

    Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • HEP-NOS antibody
    • Hepatocyte NOS antibody
    • HEPNOS antibody
    • inducible antibody
    • Inducible nitric oxide synthase antibody
    • Inducible NO synthase antibody
    • Inducible NOS antibody
    • iNOS antibody
    • MAC NOS antibody
    • Macrophage NOS antibody
    • Nitric oxide synthase 2 inducible antibody
    • Nitric oxide synthase 2 inducible macrophage antibody
    • nitric oxide synthase 2A (inducible, hepatocytes) antibody
    • Nitric oxide synthase antibody
    • Nitric oxide synthase inducible antibody
    • nitric oxide synthase, macrophage antibody
    • NOS 2 antibody
    • NOS antibody
    • Nos II antibody
    • NOS type II antibody
    • nos2 antibody
    • NOS2_HUMAN antibody
    • NOS2A antibody
    • NOS2A, Inducible, Hepatocyte antibody
    • Peptidyl-cysteine S-nitrosylase NOS2 antibody
    see all

Images

  • Western blot - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)
    Western blot - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)
    All lanes : Anti-iNOS antibody [EPR16635] (ab178945) at 1/1000 dilution

    Lane 1 : Mouse hippocampus tissue lysate
    Lane 2 : Mouse colon tissue lysate
    Lane 3 : Mouse colon cancer tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 131 kDa
    Observed band size: 131 kDa


    Exposure time: 180 seconds


    This data was developed using the same antibody clone in a different buffer formulation (ab178945).

    Blocking and diluting buffer 5% NFDM/TBST.

    iNOS is not normally expressed in the brain, but can be induced in the brain after inflammatory, infectious, or other damages (PMID: 11138926, PMID: 16156895, PMID: 10322315).

  • Western blot - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)
    Western blot - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)
    All lanes : Anti-iNOS antibody [EPR16635] (ab178945) at 1/1000 dilution

    Lane 1 : Untreated L6 (rat skeletal muscle myoblast) whole cell lysate
    Lane 2 : L6 treated with 50 ng/ml IL-1 beta, 20 ng/ml TNF-alpha and 100U/ml IFN-gamma for 24 h, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 131 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178945).

  • Western blot - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)
    Western blot - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)
    All lanes : Anti-iNOS antibody [EPR16635] (ab178945) at 1/20000 dilution

    Lane 1 : Untreated RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 2 : RAW 264.7 whole cell lysate treated with 0.1 µg/mL LPS for 6 hours

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 131 kDa
    Observed band size: 131 kDa


    Exposure time: 30 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178945).

  • Indirect ELISA - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)
    Indirect ELISA - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)

    ELISA analysis of House mouse iNOS recombinant protein at 1000 ng/mL with ab178945. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178945).

  • Western blot - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)
    Western blot - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)
    All lanes : Anti-iNOS antibody [EPR16635] (ab178945) at 1/500 dilution

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
    Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) treated with 10ug/ml lipopolysaccharides for 6 hours whole cell lysates
    Lane 3 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates
    Lane 4 : THP-1 (Human monocytic leukemia monocyte) treated with 100ng/ml lipopolysaccharides for 3 hours whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/200000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 131 kDa


    Exposure time: 3 minutes


    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178945).

  • Immunoprecipitation - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)
    Immunoprecipitation - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)

    iNOS was immunoprecipitated from 1mg of RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate treated with 1 µg/ml LPS for 24h with ab178945 at 1/100 dilution.

    Lane 1: RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 24h,10ug (Input).

    Lane 2: ab178945 IP in RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 24h.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab178945 in RAW 264.7 whole cell lysate treated with 1 µg/ml LPS for 24h.

    Western blot was performed from the immunoprecipitate using ab178945 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178945).

  • Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)
    Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)

    Clone EPR16635 (ab213987) has been successfully conjugated by Abcam. This image was generated using Anti-iNOS antibody [EPR16635] (Alexa Fluor® 647). Please refer to ab209027 for protocol details.

    Ab209027 staining iNOS in Raw264.7 cells. The lower panel shows cells treated with 1ug/ml Lipopolysaccharides (24 hr).  The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209027at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in SW480 cells fixed with 4% formaldehyde (10 min).

  • Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)
    Immunocytochemistry/ Immunofluorescence - Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)

    Immunocytochemistry/Immunofluorescence analysis of RAW 264.7 non-treated and LPS treated (0.1 µg/mL) cells labelling iNOS with ab178945 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889, Alexa Fluor® 594 conjugated anti-alpha tubulin (1/200). Nuclei counterstained with DAPI (blue).

    Secondary antibody only controls performed on non-treated and treated cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab178945).

  • Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)
    Anti-iNOS antibody [EPR16635] - BSA and Azide free (ab213987)

References (14)

Publishing research using ab213987? Please let us know so that we can cite the reference in this datasheet.

ab213987 has been referenced in 14 publications.

  • Yao M  et al. Exosomal miR-21 secreted by IL-1ß-primed-mesenchymal stem cells induces macrophage M2 polarization and ameliorates sepsis. Life Sci 264:118658 (2021). PubMed: 33115604
  • Wang L  et al. The protective role of the miR-25-mediated notch signaling pathway in the memory capacity and brain tissue of mice with central nervous system infections. Am J Transl Res 13:4835-4843 (2021). PubMed: 34150065
  • McDowell SAC  et al. Neutrophil oxidative stress mediates obesity-associated vascular dysfunction and metastatic transmigration. Nat Cancer 2:545-562 (2021). PubMed: 35122017
  • Zhou Z  et al. Withaferin A alleviates traumatic brain injury induced secondary brain injury via suppressing apoptosis in endothelia cells and modulating activation in the microglia. Eur J Pharmacol 874:172988 (2020). PubMed: 32032599
  • Zhang Z  et al. Avicularin Reduces the Expression of Mediators of Inflammation and Oxidative Stress in Bradykinin-Treated MG-63 Human Osteoblastic Osteosarcoma Cells. Med Sci Monit 26:e921957 (2020). PubMed: 32463805
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab213987.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com