Anti-Insulin degrading enzyme / IDE antibody (ab228720)
Key features and details
- Rabbit polyclonal to Insulin degrading enzyme / IDE
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Insulin degrading enzyme / IDE antibody
See all Insulin degrading enzyme / IDE primary antibodies -
Description
Rabbit polyclonal to Insulin degrading enzyme / IDE -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Zebrafish, Rhesus monkey
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Immunogen
Recombinant fragment within Human Insulin degrading enzyme/ IDE (internal sequence). The exact sequence is proprietary.
Database link: P14735 -
Positive control
- WB: HeLa cell extract; Mouse and rat brain extracts; A431, NCI-H1299, HeLa and HepG2 whole cell lysates. ICC/IF: HeLa cells. IHC-P: U87 xenograft tissue; Rat brain stem tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/500 - 1/20000. Predicted molecular weight: 118 kDa.
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| IHC-P |
1/100 - 1/1000.
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| ICC/IF |
1/100 - 1/1000.
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| Notes |
|---|
|
WB
1/500 - 1/20000. Predicted molecular weight: 118 kDa. |
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IHC-P
1/100 - 1/1000. |
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ICC/IF
1/100 - 1/1000. |
Target
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Function
Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia. -
Sequence similarities
Belongs to the peptidase M16 family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform. - Information by UniProt
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Database links
- Entrez Gene: 523752 Cow
- Entrez Gene: 477768 Dog
- Entrez Gene: 3416 Human
- Entrez Gene: 15925 Mouse
- Entrez Gene: 25700 Rat
- Entrez Gene: 698640 Rhesus monkey
- Entrez Gene: 561390 Zebrafish
- Omim: 146680 Human
see all -
Alternative names
- Abeta-degrading protease antibody
- FLJ35968 antibody
- Ide antibody
see all
Images
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Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab228720)All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab228720) at 1/2500 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Insulin degrading enzyme / IDE Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 118 kDa -
Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab228720)All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab228720) at 1/2500 dilution
Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : Insulin degrading enzyme / IDE knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 118 kDa7.5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-Insulin degrading enzyme / IDE antibody (ab228720)Ice-cold methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Insulin degrading enzyme / IDE (green) using ab228720 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin degrading enzyme / IDE antibody (ab228720)Paraffin-embedded U87 xenograft tissue stained for Insulin degrading enzyme / IDE using ab228720 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab228720)All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab228720) at 1/1000 dilution
Lane 1 : Mouse brain extract
Lane 2 : Rat brain extract
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 118 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-Insulin degrading enzyme / IDE antibody (ab228720)All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab228720) at 1/10000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 118 kDa7.5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin degrading enzyme / IDE antibody (ab228720)Paraffin-embedded rat brain stem tissue stained for Insulin degrading enzyme / IDE using ab228720 at 1/500 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab228720 has not yet been referenced specifically in any publications.