Anti-Insulin degrading enzyme / IDE antibody (ab32216)
Key features and details
- Rabbit polyclonal to Insulin degrading enzyme / IDE
- Suitable for: WB, IHC-FoFr
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Insulin degrading enzyme / IDE antibody
See all Insulin degrading enzyme / IDE primary antibodies -
Description
Rabbit polyclonal to Insulin degrading enzyme / IDE -
Host species
Rabbit -
Specificity
Replenishment batches of our polyclonal antibody, ab32216 are tested in WB. Previous batches were additionally validated in IHC-FoFr. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab133561.
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Tested applications
Suitable for: WB, IHC-FoFrmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Cat, Dog -
Immunogen
Synthetic peptide corresponding to Human Insulin degrading enzyme/ IDE aa 950 to the C-terminus.
(Peptide available asab32215) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).Can be blocked with Human Insulin degrading enzyme / IDE peptide (ab32215).
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IHC-FoFr | (2) |
1/100.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 118 kDa (predicted molecular weight: 118 kDa).Can be blocked with Human Insulin degrading enzyme / IDE peptide (ab32215). |
IHC-FoFr
1/100. |
Target
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Function
Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia. -
Sequence similarities
Belongs to the peptidase M16 family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform. - Information by UniProt
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Database links
- Entrez Gene: 523752 Cow
- Entrez Gene: 477768 Dog
- Entrez Gene: 3416 Human
- Entrez Gene: 15925 Mouse
- Entrez Gene: 25700 Rat
- Omim: 146680 Human
- SwissProt: Q24K02 Cow
- SwissProt: P14735 Human
see all -
Alternative names
- Abeta-degrading protease antibody
- FLJ35968 antibody
- Ide antibody
see all
Images
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Insulin degrading enzyme / IDE knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32216 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32216 was shown to specifically react with Insulin degrading enzyme / IDE in wild-type HAP1 cells. No band was observed when Insulin degrading enzyme / IDE knockout samples were examined. Wild-type and Insulin degrading enzyme / IDE knockout samples were subjected to SDS-PAGE. ab32216 and ab8245 (loading control to GAPDH) were diluted at 1μg/ml and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Insulin degrading enzyme / IDE antibody (ab32216)This image is courtesy of Sophie Pezet, CNRS, Paris, FranceImmunofluorescent staining for Insulin degrading enzyme/IDE in rat brain rat hippocampus using Rabbit polyclonal to Insulin degrading enzyme/IDE (ab32216). . The staining is located in the neuronal soma and is finely punctuated. The picture was acquired using the X20 objective. Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, and cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut by cryostat for use in fre floating IHC. Primary antibody ab32216 was incubated overnight at 1/100 at room temperature. Secondary antibody Alexa fluor 488 1/1000 was incubated for 2 hours at room temperature.
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All lanes : Anti-Insulin degrading enzyme / IDE antibody (ab32216) at 1 µg/ml
Lane 1 : Mouse Brain at 20 µg/ml
Lane 2 : Brain (Rat) Whole Cell Lysate - normal tissue at 20 µg
Lane 3 : Mouse Hippocampus Lysate at 20 µg
Lane 4 : Rat Hippocampus Lysate at 20 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 118 kDa
Observed band size: 118 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (70)
ab32216 has been referenced in 70 publications.
- Wang T et al. Locomotor Hyperactivity in the Early-Stage Alzheimer's Disease-like Pathology of APP/PS1 Mice: Associated with Impaired Polarization of Astrocyte Aquaporin 4. Aging Dis 13:1504-1522 (2022). PubMed: 36186142
- Zangerolamo L et al. The bile acid TUDCA reduces age-related hyperinsulinemia in mice. Sci Rep 12:22273 (2022). PubMed: 36564463
- Marmentini C et al. Aging Reduces Insulin Clearance in Mice. Front Endocrinol (Lausanne) 12:679492 (2021). PubMed: 34054736
- Lee HJ et al. Idebenone Decreases Aβ Pathology by Modulating RAGE/Caspase-3 Signaling and the Aβ Degradation Enzyme NEP in a Mouse Model of AD. Biology (Basel) 10:N/A (2021). PubMed: 34571815
- Huang HJ et al. Lactobacillus plantarum PS128 prevents cognitive dysfunction in Alzheimer's disease mice by modulating propionic acid levels, glycogen synthase kinase 3 beta activity, and gliosis. BMC Complement Med Ther 21:259 (2021). PubMed: 34627204