Recombinant Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)
![Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)](https://www.abcam.com/ps/products/133/ab133561/Images/ab133561-254641-anti-insulin-degrading-enzyme-ide-antibody-epr60982-western-blot.jpg "Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6098(2)] to Insulin degrading enzyme / IDE
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)]
See all Insulin degrading enzyme / IDE primary antibodies -
Description
Rabbit monoclonal [EPR6098(2)] to Insulin degrading enzyme / IDE -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human Insulin degrading enzyme/ IDE aa 50-150. The exact sequence is proprietary.
Database link: P14735 -
Positive control
- HeLa, HepG2, A375, and K562 cell lysates, Human colon tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.31 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6098(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 118 kDa).
|
| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 118 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Plays a role in the cellular breakdown of insulin, IAPP, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Degrades amyloid formed by APP and IAPP. May play a role in the degradation and clearance of naturally secreted amyloid beta-protein by neurons and microglia. -
Sequence similarities
Belongs to the peptidase M16 family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Cytoplasm. Cell surface. Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform. - Information by UniProt
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Database links
- Entrez Gene: 3416 Human
- Entrez Gene: 15925 Mouse
- Entrez Gene: 25700 Rat
- Omim: 146680 Human
- SwissProt: P14735 Human
- SwissProt: Q9JHR7 Mouse
- SwissProt: P35559 Rat
- Unigene: 500546 Human
see all -
Alternative names
- Abeta-degrading protease antibody
- FLJ35968 antibody
- Ide antibody
see all
Images
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Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: IDE knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133561 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab133561 was shown to specifically react with IDE in wild-type HAP1 cells. No band was observed when IDE knockout samples were examined. Wild-type and IDE knockout samples were subjected to SDS-PAGE. ab133561 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)](https://www.abcam.com/ps/products/133/ab133561/Images/ab133561-147320-anti-insulin-degrading-enzyme-ide-antibody-epr60982-western-blot.jpg)
Western blot - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)All lanes : Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A375 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 118 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)](https://www.abcam.com/ps/products/133/ab133561/Images/ab133561-147319-anti-insulin-degrading-enzyme-ide-antibody-epr60982-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Insulin degrading enzyme / IDE with ab133561 antibody at a dilution of 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)](https://www.abcam.com/ps/products/133/ab133561/Images/ab133561-147318-anti-insulin-degrading-enzyme-ide-antibody-epr60982.jpg)
OI-RD Scanning - Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561) -
![Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)](https://www.abcam.com/ps/products/133/ab133561/Images/ab133561-5-benefits-of-recombinant-antibodies.png)
Anti-Insulin degrading enzyme / IDE antibody [EPR6098(2)] (ab133561)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab133561 has been referenced in 17 publications.
- Jiang H et al. CART mitigates oxidative stress and DNA damage in memory deficits of APP/PS1 mice via upregulating ß-amyloid metabolism-associated enzymes. Mol Med Rep 23:N/A (2021). PubMed: 33604684
- Phitthayaphong P et al. Blockage of Fc Gamma Receptors Alleviates Neuronal and Microglial Toxicity Induced by Palmitic Acid. J Alzheimers Dis 82:1315-1332 (2021). PubMed: 34151811
- Lu MH et al. Intranasal Transplantation of Human Neural Stem Cells Ameliorates Alzheimer's Disease-Like Pathology in a Mouse Model. Front Aging Neurosci 13:650103 (2021). PubMed: 33776747
- Li YZ et al. Combined treatment with valproic acid and estrogen has neuroprotective effects in ovariectomized mice with Alzheimer's disease. Neural Regen Res 16:2078-2085 (2021). PubMed: 33642397
- Su Q et al. Trichostatin A ameliorates Alzheimer's disease-related pathology and cognitive deficits by increasing albumin expression and Aß clearance in APP/PS1 mice. Alzheimers Res Ther 13:7 (2021). PubMed: 33397436