Anti-Insulin Receptor beta antibody [C18C4] (ab69508)
Key features and details
- Mouse monoclonal [C18C4] to Insulin Receptor beta
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Insulin Receptor beta antibody [C18C4]
See all Insulin Receptor beta primary antibodies -
Description
Mouse monoclonal [C18C4] to Insulin Receptor beta -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human Insulin Receptor beta.
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Positive control
- MCF-7, Rat-2 and L-929 cells
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General notes
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
C18C4 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt | (1) |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
(developed by ECL) |
Notes |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa). (developed by ECL) |
Target
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Relevance
Insulin receptor mediates the biological activities of insulin by regulating multiple signaling pathways through activation of a series of phosphorylation cascades. The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a ß-a-a-ß configuration. The ß-subunit (95kDa) possesses a single transmembrane domain with tyrosine kinase acivity, whereas the a-subunit (135kDa) is completely extracellular. The alpha subunits each contain insulin binding sites and are entirely extracellular in localization. The beta subunits each possess an extracellular domain, a single transmembrane domain, and a cytoplasmic tyrosine kinase domain. Binding of insulin to the alpha subunits induces a conformation change in the receptor which activates the kinase domain, stimulating tyrosine autophosphorylation of the receptor and tyrosine phosphorylation of at least five different insulin receptor substrates designated IRS-1-4, and Shc. -
Cellular localization
Membrane; Single pass type I membrane protein. -
Database links
- Entrez Gene: 3643 Human
- Entrez Gene: 16337 Mouse
- Entrez Gene: 24954 Rat
- Omim: 147670 Human
- SwissProt: P06213 Human
- SwissProt: P15208 Mouse
- SwissProt: P15127 Rat
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Alternative names
- CD220 antibody
- HHF5 antibody
- HIR B antibody
see all
Images
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All lanes : Anti-Insulin Receptor beta antibody [C18C4] (ab69508) at 1/1000 dilution
Lane 2 : HeLa lysate (Human epithelial cell line from cervix adenocarcinoma)
Lane 3 : MCF-7 lysate (Human breast adenocarcinoma cell line)
Lane 4 : PC-12 lysate (Rat adrenal gland pheochromocytoma cell line)
Lane 5 : 3T3-L1 lysate (Mouse embryonic fibroblast cell line)
Developed using the ECL technique.
Predicted band size: 95 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?This image was generated using the ascites version of the product.
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IHC image of ab69508 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab69508, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.This image was generated using the ascites version of the product.
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Overlay histogram showing HepG2 cells stained with ab69508 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69508, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (35)
ab69508 has been referenced in 35 publications.
- Zhou Q et al. Phenylalanine impairs insulin signaling and inhibits glucose uptake through modification of IRβ. Nat Commun 13:4291 (2022). PubMed: 35879296
- Gibson C et al. Insulin-like growth factor system components expressed at the conceptus-maternal interface during the establishment of equine pregnancy. Front Vet Sci 9:912721 (2022). PubMed: 36176700
- Lin L et al. Upregulation of Klotho Aggravates Insulin Resistance in Gestational Diabetes Mellitus Trophoblast Cells. Genet Res (Camb) 2022:1500768 (2022). PubMed: 36325267
- Wang W et al. Pten Regulates Cardiomyocyte Differentiation by Modulating Non-CG Methylation via Dnmt3. Adv Sci (Weinh) 8:e2100849 (2021). PubMed: 34247447
- Stewart CM et al. Ebola virus triggers receptor tyrosine kinase-dependent signaling to promote the delivery of viral particles to entry-conducive intracellular compartments. PLoS Pathog 17:e1009275 (2021). PubMed: 33513206