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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)

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Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
  • Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
  • Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
  • Immunoprecipitation - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
  • Flow Cytometry (Intracellular) - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
  • Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17338] to Integrin alpha 2 - Low endotoxin, Azide free
  • Suitable for: WB, Flow Cyt (Intra), IHC-P, IP, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free
    See all Integrin alpha 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR17338] to Integrin alpha 2 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt (Intra), IHC-P, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549, A431, 293T,T-47D, C6 and NIH/3T3 whole cell lysates, human fetal brain and fetal heart, mouse heart and kidney, and rat spleen tissue lysates. IHC-P: Human colon tissue, human squamous cell carcinoma of cervix tissue, mouse kidney tissue and rat colon tissues. ICC/IF: Wild-type HAP1, PC-3 and MCF7 cells. Flow Cyt (intra): A549 cells. IP: T-47D whole cell extract.

  • General notes

    ab222377 is the carrier-free version of ab181548.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab222377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

This product gave a positive signal in HAP1-ITGA2 wild-type cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).
Notes
WB
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

This product gave a positive signal in HAP1-ITGA2 wild-type cells fixed with 4% formaldehyde (10 min) and 100% methanol (5 min).

Target

  • Function

    Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.

  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.

  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • BR antibody
      • CD 49b antibody
      • CD49 antigen like family member B antibody
      • CD49 antigen-like family member B antibody
      • CD49b antibody
      • CD49b antigen antibody
      • Collagen receptor antibody
      • DX5 antibody
      • Glycoprotein Ia deficiency included antibody
      • GP Ia antibody
      • GP Ia deficiency, included antibody
      • GPIa antibody
      • HPA 5 included antibody
      • HPA5 included antibody
      • Human platelet alloantigen system 5 antibody
      • Integrin alpha 2 antibody
      • Integrin alpha II antibody
      • Integrin alpha-2 antibody
      • Integrin, alpha 2 (CD49B alpha 2 subunit of VLA 2 receptor) antibody
      • ITA2_HUMAN antibody
      • ITGA2 antibody
      • Platelet alloantigen Br(a), included antibody
      • Platelet antigen Br antibody
      • Platelet glycoprotein GPIa antibody
      • Platelet glycoprotein Ia antibody
      • Platelet glycoprotein Ia/IIa antibody
      • Platelet membrane glycoprotein Ia antibody
      • Platelet receptor for collagen, deficiency of, included antibody
      • Very late activation protein 2 receptor alpha 2 subunit antibody
      • VLA 2 alpha chain antibody
      • VLA 2 antibody
      • VLA 2 subunit alpha antibody
      • VLA-2 subunit alpha antibody
      • VLA2 antibody
      • VLA2 receptor alpha 2 subunit antibody
      • VLAA2 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
      Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)

      ab181548 staining Integrin α2 in wild-type HAP1 cells (top panel) and Integrin α2 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab181548 at 1μg/ml concentration and ab7291 at 1ug/ml concentration overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to Mouse IgG (Alexa Fluor® 594) (ab150120) at 2ug/ml (shown in pseudo-color red). Nuclear DNA was labelled in blue with DAPI.


      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181548).

    • Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
      Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling integrin alpha 2 with ab181548 at 1/100 dilution, followed by Goat anti-rabbit IAlexa Fluor® 488 (IgG) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing membrane staining on MCF7 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse AlexaFluor®594 secondary antibody) at 1/500 dilution (red).
      The negative controls are as follows:-
      -ve control 1 - ab181548 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

       

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181548).

    • Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
      Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-3 (Human prostate adenocarcinoma cell line) cells labeling integrin alpha 2 with ab181548 at 1/100 dilution, followed by Goat anti-rabbit IAlexa Fluor® 488 (IgG) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing membrane and weakly cytoplasmic staining on PC-3 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse AlexaFluor®594 secondary antibody) at 1/500 dilution (red).

      The negative controls are as follows:-
      -ve control 1 - ab181548 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

       

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181548).

    • Immunoprecipitation - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
      Immunoprecipitation - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)

      Integrin alpha 2 was immunoprecipitated from 1mg of T-47D (Human ductal breast epithelial tumor cell line) whole cell extract with ab181548 at 1/150 dilution. Western blot was performed using ab181548 at 1/20,000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: T-47D whole cell extract  Lane 2: PBS instead of T-47D whole cell extract.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181548).

    • Flow Cytometry (Intracellular) - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
      Flow Cytometry (Intracellular) - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)

      Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed A549 (Human lung carcinoma) cells labeling integrin alpha 2 with ab181549 at 1/160 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181548). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)

      Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Integrin alpha 2 with ab181548 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) at 1/500 dilution. Membrane staining on epithelial cells of Rat colon tissue is observed. Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181548).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)

      Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Integrin alpha 2 with ab181548 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) at 1/500 dilution. Membrane and weak cytoplasmic staining on epithelial cells of Mouse kidney tubule is observed. Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181548).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)

      Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling Integrin alpha 2 with ab181548 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) at 1/500 dilution. Membrane and weak cytoplasmic staining on epithelial cells of human squamous cell carcinoma of cervix tissue is observed. Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181548).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Integrin alpha 2 with ab181548 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) at 1/500 dilution. Membrane and weak cytoplasmic staining on epithelial cells of human colon is observed. Counter stained with Hematoxylin.

      Negative control: Used PBS instead of primary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181548).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)
      Anti-Integrin alpha 2 antibody [EPR17338] - Low endotoxin, Azide free (ab222377)

    References (1)

    Publishing research using ab222377? Please let us know so that we can cite the reference in this datasheet.

    ab222377 has been referenced in 1 publication.

    • Ghosh S  et al. Endoplasmic Reticulum Resident Heat Shock Protein 90 (Hsp90) Isoform Glucose-Regulated Protein 94 (Grp94) Regulates Cell Polarity and Cancer Cell Migration by Affecting Intracellular Transport. J Biol Chem N/A:N/A (2016). WB ; Human . PubMed: 26872972

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