Recombinant Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)
![Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)](https://www.abcam.com/ps/products/81/ab81280/Images/ab81280-253282-ab81280KOWB.jpg "Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1355Y] to Integrin alpha 4/CD49D
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Integrin alpha 4/CD49D antibody [EPR1355Y]
See all Integrin alpha 4/CD49D primary antibodies -
Description
Rabbit monoclonal [EPR1355Y] to Integrin alpha 4/CD49D -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human Integrin alpha 4/CD49D aa 1000-1100 (C terminal). The exact sequence is proprietary.
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Positive control
- Jurkat whole cell lysate (ab7899)
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1355Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/2000. Predicted molecular weight: 150 kDa.
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| Notes |
|---|
|
WB
1/1000 - 1/2000. Predicted molecular weight: 150 kDa. |
Target
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Function
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Domain
The SG1 motif is involved in binding to chondroitin sulfate glycosaminoglycan and cell adhesion. -
Post-translational
modificationsPhosphorylation on Ser-1027 inhibits PXN binding. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3676 Human
- Entrez Gene: 16401 Mouse
- Omim: 192975 Human
- SwissProt: P13612 Human
- SwissProt: Q00651 Mouse
- Unigene: 440955 Human
- Unigene: 31903 Mouse
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Alternative names
- 269C wild type antibody
- Antigen CD49D, alpha 4 subunit of VLA 4 receptor antibody
- CD49 antigen like family member D antibody
see all
Images
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Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)
Lane 1: Integrin alpha 4 knockout HAP1 cell lysate (20 µg)
Lane 2: Wild-type HAP1 cell lysate (20 µg)
Lanes 1 and 2: Merged signal (red and green). Green - ab81280 observed at 150 kDa. Red - loading control, ab8226, observed at 42 kDa.
ab81280 was shown to recognize Integrin alpha 4 in wild-type HAP1 cells along with additional cross-reactive bands. No bands were observed when Integrin alpha 4 knockout samples were used. Wild-type and Integrin alpha 4 knockout samples were subjected to SDS-PAGE. ab81280 and ab8226 (loading control to beta actin) were diluted 1/2000 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging. -
![Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)](https://www.abcam.com/ps/products/81/ab81280/Images/ab81280-282047-ab81280.jpg)
Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)All lanes : Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280) at 1/1000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Mouse spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 150 kDa
Observed band size: 150 kDaLanes 1 - 2: Merged signal (red and green). Green - ab81280 observed at 150 kDa. Red - loading control, ab8245, observed at 37 kDa.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab81280 and ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at a 1:10000 dilution for 1hr at room temperature and then imaged.
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![Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)](https://www.abcam.com/ps/products/81/ab81280/Images/Integrin-alpha-4-Primary-antibodies-ab81280-1.jpg)
Western blot - Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280) at 1/2000 dilution + Jurkat cell lysate at 10 µg
Secondary
HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 150 kDa
Observed band size: 150 kDa -
![Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)](https://www.abcam.com/ps/products/81/ab81280/Images/ab81280-5-benefits-of-recombinant-antibodies.png)
Anti-Integrin alpha 4/CD49D antibody [EPR1355Y] (ab81280)
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab81280 has been referenced in 9 publications.
- Yan J et al. Integrative transcriptomic and proteomic analysis reveals CD9/ITGA4/PI3K-Akt axis mediates trabecular meshwork cell apoptosis in human glaucoma. J Cell Mol Med 24:814-829 (2020). PubMed: 31680442
- Ohkuro M et al. Calreticulin and integrin alpha dissociation induces anti-inflammatory programming in animal models of inflammatory bowel disease. Nat Commun 9:1982 (2018). PubMed: 29773794
- Mishra P et al. ADHFE1 is a breast cancer oncogene and induces metabolic reprogramming. J Clin Invest 128:323-340 (2018). PubMed: 29202474
- Zhang YL et al. SPON2 Promotes M1-like Macrophage Recruitment and Inhibits Hepatocellular Carcinoma Metastasis by Distinct Integrin-Rho GTPase-Hippo Pathways. Cancer Res 78:2305-2317 (2018). PubMed: 29440144
- van de Weijer ML et al. Multiple E2 ubiquitin-conjugating enzymes regulate human cytomegalovirus US2-mediated immunoreceptor downregulation. J Cell Sci 130:2883-2892 (2017). PubMed: 28743740
- de la Rica L et al. NF-?B-direct activation of microRNAs with repressive effects on monocyte-specific genes is critical for osteoclast differentiation. Genome Biol 16:2 (2015). WB . PubMed: 25601191
- Bing T et al. Facile Discovery of Cell-Surface Protein Targets of Cancer Cell Aptamers. Mol Cell Proteomics 14:2692-700 (2015). WB ; Human . PubMed: 26199357
- Buggins AG et al. Evidence for a macromolecular complex in poor prognosis CLL that contains CD38, CD49d, CD44 and MMP-9. Br J Haematol 154:216-22 (2011). IF, WB ; Human . PubMed: 21569005
- Conant CG et al. Well plate-coupled microfluidic devices designed for facile image-based cell adhesion and transmigration assays. J Biomol Screen 15:102-6 (2010). PubMed: 19965806