Recombinant Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)
![Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)](https://www.abcam.com/ps/products/124/ab124924/Images/ab124924-449294-anti-integrin-alpha-6-antibody-epr5578-western-blot-sw480-wt-sw480-itga6-knockout-hek293.jpg "Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5578] to Integrin alpha 6
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Integrin alpha 6 antibody [EPR5578]
See all Integrin alpha 6 primary antibodies -
Description
Rabbit monoclonal [EPR5578] to Integrin alpha 6 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Integrin alpha 6 aa 100-200. The exact sequence is proprietary.
Database link: P23229 -
Positive control
- SW480, HEK293, JAR, HT1376, and Human platelet lysates; Human kidney tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.29 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5578 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124924 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 126 kDa (predicted molecular weight: 126 kDa).
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 126 kDa (predicted molecular weight: 126 kDa). |
Target
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Function
Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760). -
Tissue specificity
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). -
Involvement in disease
Epidermolysis bullosa letalis, with pyloric atresia -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Post-translational
modificationsIsoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
Palmitoylation by DHHC3 enhances stability and cell surface expression. -
Cellular localization
Cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3655 Human
- Omim: 147556 Human
- SwissProt: P23229 Human
- Unigene: 133397 Human
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Alternative names
- Alpha6p antibody
- CD49 antigen-like family member F antibody
- CD49f antibody
see all
Images
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Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)All lanes : Anti-Integrin alpha 6 antibody [EPR5578] (ab124924) at 1/1000 dilution
Lane 1 : Wild-type SW480 cell lysate
Lane 2 : ITGA6 knockout SW480 cell lysate
Lane 3 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 126 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab124924 observed at 130 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab124924 was shown to react with Integrin alpha 6 in wild-type SW480 cells in western blot with loss of signal observed in ITGA6 knockout cell line ab265838 (knockout cell lysate ab256957). Wild-type and ITGA6 knockout SW480 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab124924 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)](https://www.abcam.com/ps/products/124/ab124924/Images/ab124924-254377-anti-integrin-alpha-6-antibody-epr5578-western-blot.jpg)
Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)All lanes : Anti-Integrin alpha 6 antibody [EPR5578] (ab124924) at 1/10000 dilution (purified)
Lane 1 : HT-1376 cell lysate
Lane 2 : JAR cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution
Predicted band size: 126 kDa
Observed band size: 126 kDaBlocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)](https://www.abcam.com/ps/products/124/ab124924/Images/ab124924-138938-anti-integrin-alpha-6-antibody-epr5578-western-blot.jpg)
Western blot - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)All lanes : Anti-Integrin alpha 6 antibody [EPR5578] (ab124924) at 1/1000 dilution
Lane 1 : JAR cell lysate
Lane 2 : HT1376 cell lysate
Lane 3 : Human platelet lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 126 kDaSecondary antibody - anti-rabbit HRP (ab6721)
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![OI-RD Scanning - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)](https://www.abcam.com/ps/products/124/ab124924/Images/ab124924-138936-anti-integrin-alpha-6-antibody-epr5578.jpg)
OI-RD Scanning - Anti-Integrin alpha 6 antibody [EPR5578] (ab124924) -
![Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)](https://www.abcam.com/ps/products/124/ab124924/Images/ab124924-5-benefits-of-recombinant-antibodies.png)
Anti-Integrin alpha 6 antibody [EPR5578] (ab124924)
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab124924 has been referenced in 5 publications.
- Kurita T et al. Pathological mechanisms of laryngeal papillomatosis based on laryngeal epithelial characteristics. Laryngoscope Investig Otolaryngol 4:89-94 (2019). PubMed: 30828624
- Hu T et al. Integrin a6/Akt/Erk signaling is essential for human breast cancer resistance to radiotherapy. Sci Rep 6:33376 (2016). PubMed: 27624978
- Ocampo A et al. In Vivo Amelioration of Age-Associated Hallmarks by Partial Reprogramming. Cell 167:1719-1733.e12 (2016). PubMed: 27984723
- Suprynowicz FA et al. Conditionally reprogrammed cells represent a stem-like state of adult epithelial cells. Proc Natl Acad Sci U S A 109:20035-40 (2012). WB ; Human . PubMed: 23169653
- Kariya Y & Gu J N-glycosylation of ß4 integrin controls the adhesion and motility of keratinocytes. PLoS One 6:e27084 (2011). WB . PubMed: 22073258