Anti-Integrin alpha V antibody [272-17E6] - BSA and Azide free (ab16821)

Vielen Dank für Ihre Anfrage.

Das Labor, von welchem wir diesen Antikörper beziehen, hat uns tatsächlich informiert, dass dieser Antikörper nicht auf Paraffinschnitten funktionieren sollte. Dies ist eine Information von dem Labor, welches den Antikörper ursprünglich hergestellt hat.

Ich entschuldige mich für diese Umstände, und danke Ihnen, unsere Aufmerksamkeit auf dies gelenkt zu haben. Ich habe das Datenblatt aktualisiert und betroffene Kunden werden informiert.

Kann ich Ihnen vielleicht andersweitig helfen? Wir haben zwei polyklonale Antikörper, welche auf Paraffinschnitten validiert wurden: ab76609 und ab117611.
https://www.abcam.com/Integrin-alpha-V-antibody-ab76609.html (or use the following: https://www.abcam.com/Integrin-alpha-V-antibody-ab76609.html).
https://www.abcam.com/Integrin-alpha-V-antibody-ab117611.html (or use the following: https://www.abcam.com/Integrin-alpha-V-antibody-ab117611.html).

Bitte zögern Sie nicht, uns wieder zu kontaktieren, sollten Sie weitere Fragen oder Bedenken haben. Ich wünsche Ihnen ein schönes Wochenende.

The exact target epitope was not characterized in-house. Please see the references listed above for more details.
We do not have information on this product's reactivity with mouse as this antibody has, to our knowledge, not been tested on mouse samples.

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I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

I am sorry to confirm that we don't have any different lots in stock at the moment. I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this batch. Regrettably, I can suggest you have received a bad vial on this occasion.

Therefore, we would be pleased to provide a replacement from the same lot, which will still be covered by our guarantee. Alternatively, I fully understand your concerns and you prefer a credit note in this case, I will be pleased to arrange this for you.

I hope this will be helpful. I look forward to hearing from you with details of how you would like to proceed.

I am sorry about the issues you have been having. Thank you for bringing this to our attention.

I would very much like to help you with this. However to do that I need the particulars of your protocol. This information is important not only in helping us understand the problems that you are experiencing but in our internal testing as well.

Could you tell me the tissue types and lysis buffer that you have used?

Have you reduced and denatured your samples? Which method/buffers did you use?

How much total protein did you use per well?

Did you load a loading control? Which LC did you use? What were the results?

Did you load a positive control? Which PC did you use? What were the results?

What membrane type did you transfer to?

What blocking steps did you use?

What dilutions of this product did you use? How long and at what temperature was this antibody incubated?

What was the secondary antibody that you have used? How long and at what temperature was this antibody incubated?

Have you run a no primary antibody control blot? What were the results?

What is the wash buffer that you use?

Could you provide the Abcam order confirmation number or PO number used to purchase this?

Thank you very much for taking the time to answer these questions. Please contact me if you have any questions or concerns.

Thank you very much for contacting us - and sending the enquiry as a table. That clear overview was very helpful :-) As you may know, we have not tested the individual samples for their expression of the mentioned targets. However, according to the literature and other sources such as http://www.proteinatlas.org/ these tissues exhibit high expression of these proteins and thus are suitable controls. Cat# Product Positive control Slide ab27526 Anti-Notch1 antibody Breast carcinoma ab4697, ab4698 (but also ab4701, ab4700, ab4696) ab7970 Anti-NFkB p65 antibody Spleen (or skin) ab4372 (or ab4365, ab4417)　 ab10800 Anti-Integrin alpha 2 antibody Bladder, gall bladder, kidney　 ab4287, ab4330, ab4347　 ab20142 Anti-Integrin alpha 6 antibody Bladder, gall bladder, oesophagus　 ab4287, ab4330, ab4404　 ab16821 Anti-Integrin alpha V antibody Human breast carcinoma ab4697, ab4698 (but also ab4701, ab4700, ab4696)　 ab52971 Anti-Integrin beta 1 antibody Human breast carcinoma ab4697, ab4698 (but also ab4701, ab4700, ab4696)　 ab75872 Anti-Integrin beta 3 antibody Human spleen lysate and human tonsil tissue. ab4372, ab5146 ab79007 Anti-Integrin alpha 1 + beta 1 antibody Rat gut, heart and lung tissue. ab4620, ab4617　 ab30483 Anti-Integrin alpha 2+beta 1 antibody Bladder, gall bladder,breast carcinoma ab4287, ab4330, ab4697, ab4698 (but also ab4701, ab4700, ab4696)　 ab16822 Anti-Integrin alpha 2b + beta 3 antibody Spleen, kidney　 ab4372, ab4347　 ab78289 Anti-Integrin alpha V beta 3 antibody Bladder, oesophagus　 ab4287, ab4404　 All the above mentioned antibodies are guaranteed to work in immunohistochemistry on paraffin-embedded sections as stated on the datasheet. However, we do not currently have an image of ab10800, ab16821, ab30483, ab16822, and ab78289 in IHC-P. Therefore, I can offer a discount off a future purchase if you buy one of these antibodies now, use it IHC-P and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody each. If you decide to go ahead and purchase this product, please contact our Scientific Support team prior to the purchase for more details. I hope this information is helpful to you. Please do not hesitate to contact me if you need any further advice or information in this regard.