Recombinant Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16800] to Integrin alpha V
- Suitable for: WB, IP, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Integrin alpha V antibody [EPR16800]
See all Integrin alpha V primary antibodies -
Description
Rabbit monoclonal [EPR16800] to Integrin alpha V -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HUVEC, HT-29, A549, C6 and NIH/3T3 whole cell lysates; Human fetal kidney and fetal brain lysates; Mouse brain, kidney and spleen lysates; Rat brain and kidney lysates. IHC: Human kidney, human transitional cell carcinoma of bladder and mouse kidney tissues. ICC/IF: A549 cells. IP: A549 whole cell extract.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16800 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 647 Anti-Integrin alpha V antibody [EPR16800] (ab204684)
- HRP Anti-Integrin alpha V antibody [EPR16800] (ab205474)
- Alexa Fluor® 594 Anti-Integrin alpha V antibody [EPR16800] (ab207286)
- PE Anti-Integrin alpha V antibody [EPR16800] (ab211845)
- Anti-Integrin alpha V antibody [EPR16800] - Low endotoxin, Azide free (ab222222)
- Anti-Integrin alpha V antibody [EPR16800] - BSA and Azide free (ab271932)
- Alexa Fluor® 555 Anti-Integrin alpha V antibody [EPR16800] (ab275351)
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179475 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
1/5000. Detects a band of approximately 125, 135 kDa (predicted molecular weight: 116 kDa).
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IP |
1/40.
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ICC/IF | (3) |
1/500.
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IHC-P | (3) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/5000. Detects a band of approximately 125, 135 kDa (predicted molecular weight: 116 kDa). |
IP
1/40. |
ICC/IF
1/500. |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3685 Human
- Entrez Gene: 16410 Mouse
- Entrez Gene: 296456 Rat
- Omim: 193210 Human
- SwissProt: P06756 Human
- SwissProt: P43406 Mouse
- Unigene: 436873 Human
- Unigene: 227 Mouse
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Alternative names
- antigen identified by monoclonal antibody L230 antibody
- CD 51 antibody
- CD51 antibody
see all
Images
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All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/1000 dilution
Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate. with NFDM/TBST
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate treated with PNGase F. with NFDM/TBST
Lysates/proteins at 15 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated.)
Predicted band size: 116 kDa
Observed band size: 116.135 kDa why is the actual band size different from the predicted?
Exposure time: 20 seconds -
All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/1000 dilution
Lane 1 : Untreated K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate. with NFDM/TBST
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) treated with PNGase F whole cell lysate. with NFDM/TBST
Lysates/proteins at 15 µg per lane.
Blocking peptides at 5 % per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated.)
Predicted band size: 116 kDa
Observed band size: 116.135 kDa why is the actual band size different from the predicted?
Exposure time: 80 seconds -
All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/50000 dilution
Lane 1 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysates
Lane 2 : HT-29 (Human colorectal adenocarcinoma cells) whole cell lysates
Lane 3 : A549 (Human lung carcinoma) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 116 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?Blocking/dilution buffer: 5% NFDM/TBST.
ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary antibody at 1/500 dilution.
Membrane and weak cytoplasm staining on human transitional cell carcinoma of bladder is observed. Counterstained with hematoxylin.
Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Integrin alpha V was immunoprecipitated from 1 mg of A549 (Human lung carcinoma) whole cell extract with ab179475 at 1/40 dilution. Western blot analysis was performed from the immunoprecipitate using ab179475 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: A549 whole cell extract.
Lane 2: PBS instead of A549 whole cell extract.
Blocking/Dilution buffer: 5% NFDM/TBST.ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.
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Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma) cells labeling Integrin alpha V with ab179475 at 1/500 dilution, followed by Goat anti-rabbit Alexa Fluor® 488 (IgG) (ab150077) secondary antibody at 1/400 dilution (green).
Confocal image showing membrane and cytoplasm staining on A549 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse AlexaFluor®594 secondary antibody) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab179475 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
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All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/5000 dilution
Lane 1 : Human fetal kidney lysates
Lane 2 : Human fetal brain lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 116 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?Blocking/dilution buffer: 5% NFDM/TBST.
ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary antibody at 1/500 dilution.
Membrane and cytoplasm staining on human kidney tubules is observed. Counterstained with hematoxylin.
Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/5000 dilution
Lane 1 : Mouse brain lysates
Lane 2 : Mouse kidney lysates
Lane 3 : Mouse spleen lysates
Lane 4 : Rat brain lysates
Lane 5 : Rat kidney lysates
Lane 6 : C6 (Rat glial tumor cells) whole cell lysates
Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates
Lane 8 : Raji (Human Burkitt's lymphoma cell line) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 116 kDa
Observed band size: 125,135 kDa why is the actual band size different from the predicted?Blocking/dilution buffer: 5% NFDM/TBST.
ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary at 1/500 dilution.
Membrane and cytoplasm staining on mouse kidney tubule and glomerulus is observed. Counterstained with hematoxylin.
Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)This image is courtesy of an anonymous Abreview.All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/2000 dilution (8 hours at 4°C)
All lanes : Mouse skin whole cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG(H+L)-HRP at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 116 kDa
Exposure time: 25 secondsDiluent: 2% BSA in 1X TBST.
Blocked using 5% BSA for 1 hour at RT.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (57)
ab179475 has been referenced in 57 publications.
- Liu JJ et al. RGD-functionalised melanin nanoparticles for intraoperative photoacoustic imaging-guided breast cancer surgery. Eur J Nucl Med Mol Imaging 49:847-860 (2022). PubMed: 34505945
- Xue Y et al. Myokine Irisin promotes osteogenesis by activating BMP/SMAD signaling via αV integrin and regulates bone mass in mice. Int J Biol Sci 18:572-584 (2022). PubMed: 35002510
- Yang RQ et al. Biodegradable Nanoprobe for NIR-II Fluorescence Image-Guided Surgery and Enhanced Breast Cancer Radiotherapy Efficacy. Adv Sci (Weinh) 9:e2104728 (2022). PubMed: 35170876
- Shen R et al. Long noncoding RNA LINC00518 contributes to proliferation and metastasis in lung adenocarcinoma via the miR-335-3p/CTHRC1 Axis. Cell Death Discov 8:98 (2022). PubMed: 35246517
- Sato N et al. Yin Yang 1 regulates ITGAV and ITGB1, contributing to improved prognosis of colorectal cancer. Oncol Rep 47:N/A (2022). PubMed: 35266011