Name: Anti-Integrin alpha V antibody [EPR16800]
Brand: RabMAb
SKU: ab179475
Rating: 5 (10 reviews)

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Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16800] to Integrin alpha V
Suitable for: WB, IP, ICC/IF, IHC-P
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free HRP PE

Product name

Anti-Integrin alpha V antibody [EPR16800]
See all Integrin alpha V primary antibodies
Description

Rabbit monoclonal [EPR16800] to Integrin alpha V
Host species

Rabbit
Tested applications

Suitable for: WB, IP, ICC/IF, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HUVEC, HT-29, A549, C6 and NIH/3T3 whole cell lysates; Human fetal kidney and fetal brain lysates; Mouse brain, kidney and spleen lysates; Rat brain and kidney lysates. IHC: Human kidney, human transitional cell carcinoma of bladder and mouse kidney tissues. ICC/IF: A549 cells. IP: A549 whole cell extract.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16800
Isotype

IgG
Research areas

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(4)	1/5000. Detects a band of approximately 125, 135 kDa (predicted molecular weight: 116 kDa).
IP		1/40.
ICC/IF	(3)	1/500.
IHC-P	(3)	1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Notes
WB 1/5000. Detects a band of approximately 125, 135 kDa (predicted molecular weight: 116 kDa).
IP 1/40.
ICC/IF 1/500.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Function

The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Sequence similarities

Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Cellular localization

Membrane.
Target information above from: UniProt accession P06756 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 3685 Human
- Entrez Gene: 16410 Mouse
- Entrez Gene: 296456 Rat
- Omim: 193210 Human
- SwissProt: P06756 Human
- SwissProt: P43406 Mouse
- Unigene: 436873 Human
- Unigene: 227 Mouse
Alternative names
- antigen identified by monoclonal antibody L230 antibody
- CD 51 antibody
- CD51 antibody
- DKFZp686A08142 antibody
- Integrin alpha five antibody
- integrin alpha V beta 3 antibody
- Integrin alpha-5 antibody
- integrin alpha-V antibody
- Integrin alpha-V light chain antibody
- integrin alphaVbeta3 antibody
- integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) antibody
- ITAV_HUMAN antibody
- ITGAV antibody
- MSK 8 antibody
- Msk8 antibody
- Vitronectin receptor subunit alpha antibody
- VNRA antibody
- VTNR antibody
see all

Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/1000 dilution

Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate. with NFDM/TBST
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate treated with PNGase F. with NFDM/TBST

Lysates/proteins at 15 µg per lane.

Blocking peptides at 5 % per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated.)

Predicted band size: 116 kDa
Observed band size: 116.135 kDa why is the actual band size different from the predicted?

Exposure time: 20 seconds
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/1000 dilution

Lane 1 : Untreated K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate. with NFDM/TBST
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) treated with PNGase F whole cell lysate. with NFDM/TBST

Lysates/proteins at 15 µg per lane.

Blocking peptides at 5 % per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated.)

Predicted band size: 116 kDa
Observed band size: 116.135 kDa why is the actual band size different from the predicted?

Exposure time: 80 seconds
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/50000 dilution

Lane 1 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysates
Lane 2 : HT-29 (Human colorectal adenocarcinoma cells) whole cell lysates
Lane 3 : A549 (Human lung carcinoma) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 116 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?

Blocking/dilution buffer: 5% NFDM/TBST.

ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary antibody at 1/500 dilution.

Membrane and weak cytoplasm staining on human transitional cell carcinoma of bladder is observed. Counterstained with hematoxylin.

Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

Integrin alpha V was immunoprecipitated from 1 mg of A549 (Human lung carcinoma) whole cell extract with ab179475 at 1/40 dilution. Western blot analysis was performed from the immunoprecipitate using ab179475 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1: A549 whole cell extract.

Lane 2: PBS instead of A549 whole cell extract.

Blocking/Dilution buffer: 5% NFDM/TBST.

ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.
Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma) cells labeling Integrin alpha V with ab179475 at 1/500 dilution, followed by Goat anti-rabbit Alexa Fluor^® 488 (IgG) (ab150077) secondary antibody at 1/400 dilution (green).

Confocal image showing membrane and cytoplasm staining on A549 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse AlexaFluor^®594 secondary antibody) at 1/500 dilution (red).

The negative controls are as follows:

-ve control 1: ab179475 at 1/500 dilution followed by ab150120 (AlexaFluor^®594 Goat anti-Mouse secondary) at 1/500 dilution.

-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor^®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/5000 dilution

Lane 1 : Human fetal kidney lysates
Lane 2 : Human fetal brain lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 116 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?

Blocking/dilution buffer: 5% NFDM/TBST.

ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary antibody at 1/500 dilution.

Membrane and cytoplasm staining on human kidney tubules is observed. Counterstained with hematoxylin.

Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/5000 dilution

Lane 1 : Mouse brain lysates
Lane 2 : Mouse kidney lysates
Lane 3 : Mouse spleen lysates
Lane 4 : Rat brain lysates
Lane 5 : Rat kidney lysates
Lane 6 : C6 (Rat glial tumor cells) whole cell lysates
Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates
Lane 8 : Raji (Human Burkitt's lymphoma cell line) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 116 kDa
Observed band size: 125,135 kDa why is the actual band size different from the predicted?

Blocking/dilution buffer: 5% NFDM/TBST.

ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary at 1/500 dilution.

Membrane and cytoplasm staining on mouse kidney tubule and glomerulus is observed. Counterstained with hematoxylin.

Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)This image is courtesy of an anonymous Abreview.

All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/2000 dilution (8 hours at 4°C)

All lanes : Mouse skin whole cell lysate

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG(H+L)-HRP at 1/5000 dilution

Developed using the ECL technique.

Predicted band size: 116 kDa

Exposure time: 25 seconds

Diluent: 2% BSA in 1X TBST.

Blocked using 5% BSA for 1 hour at RT.

See Abreview
Anti-Integrin alpha V antibody [EPR16800] (ab179475)

Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab179475? Please let us know so that we can cite the reference in this datasheet.

ab179475 has been referenced in 57 publications.

Liu JJ et al. RGD-functionalised melanin nanoparticles for intraoperative photoacoustic imaging-guided breast cancer surgery. Eur J Nucl Med Mol Imaging 49:847-860 (2022). PubMed: 34505945
Xue Y et al. Myokine Irisin promotes osteogenesis by activating BMP/SMAD signaling via αV integrin and regulates bone mass in mice. Int J Biol Sci 18:572-584 (2022). PubMed: 35002510
Yang RQ et al. Biodegradable Nanoprobe for NIR-II Fluorescence Image-Guided Surgery and Enhanced Breast Cancer Radiotherapy Efficacy. Adv Sci (Weinh) 9:e2104728 (2022). PubMed: 35170876
Shen R et al. Long noncoding RNA LINC00518 contributes to proliferation and metastasis in lung adenocarcinoma via the miR-335-3p/CTHRC1 Axis. Cell Death Discov 8:98 (2022). PubMed: 35246517
Sato N et al. Yin Yang 1 regulates ITGAV and ITGB1, contributing to improved prognosis of colorectal cancer. Oncol Rep 47:N/A (2022). PubMed: 35266011

View all Publications for this product

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1-10 of 10 Abreviews or Q&A

Application

Western blot

Sample

Mouse Tissue lysate - whole (heart)

Gel Running Conditions

Reduced Denaturing (4-12)

Loading amount

10 µg

Specification

heart

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 17 2021

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Human prostate tissue)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris-EDTA buffer (pH 9.0)

Permeabilization

Yes - 0.2% Triton X-100 in PBS

Specification

Human prostate tissue

Blocking step

Blocking according to ab210061 - Double stain IHC kit plus goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C

Fixative

No fixation

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

Submitted Aug 11 2021

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (Mice prostate tissue)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris-EDTA buffer (pH 9.0)

Permeabilization

Yes - 0.2% Triton X-100 in PBS

Specification

Mice prostate tissue

Blocking step

Blocking according to ab210061 - Double stain IHC kit plus goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C

Fixative

No fixation

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

Submitted Aug 11 2021

Application

Western blot

Sample

Rat Cell lysate - whole cell (Rat hepatocytes)

Gel Running Conditions

Reduced Denaturing (8% SDS-PAGE)

Loading amount

35 µg

Specification

Rat hepatocytes

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

Submitted Aug 11 2021

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (RM-1 - mouse prostate cancer cells)

Permeabilization

Yes - 0.2% Triton X-100 in PBS

Specification

RM-1 - mouse prostate cancer cells

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

Submitted Dec 03 2020

Application

Immunocytochemistry/ Immunofluorescence

Sample

Rat Cell (Rat hepatocytes)

Permeabilization

Yes - 0.2% Triton X-100 in PBS

Specification

Rat hepatocytes

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

Submitted Sep 10 2020

Application

Western blot

Sample

Human Cell lysate - whole cell (22Rv1 - prostate cancer cells)

Gel Running Conditions

Reduced Denaturing (8% SDS-PAGE)

Loading amount

25 µg

Specification

22Rv1 - prostate cancer cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

Submitted Aug 31 2020

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (PC-3 - prostate cancer cells)

Permeabilization

Yes - 0.2% Triton X-100 in PBS

Specification

PC-3 - prostate cancer cells

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

Submitted Aug 28 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Human skin SCC)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate buffer

Permeabilization

Specification

Human skin SCC

Blocking step

Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Yong Sun Lee

Verified customer

Submitted Jun 27 2018

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Mouse skin)

Gel Running Conditions

Reduced Denaturing (8%)

Loading amount

40 µg

Specification

Mouse skin

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 20 2018

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (57)

Customer reviews and Q&As

Western blot abreview for Anti-Integrin alpha V antibody [EPR16800]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha V antibody [EPR16800]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha V antibody [EPR16800]

Western blot abreview for Anti-Integrin alpha V antibody [EPR16800]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Integrin alpha V antibody [EPR16800]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Integrin alpha V antibody [EPR16800]

Western blot abreview for Anti-Integrin alpha V antibody [EPR16800]

Immunocytochemistry/ Immunofluorescence abreview for Anti-Integrin alpha V antibody [EPR16800]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha V antibody [EPR16800]

Western blot abreview for Anti-Integrin alpha V antibody [EPR16800]