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RecombinantRabMAb

Recombinant Anti-Integrin alpha V antibody [EPR16800] (ab179475)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (10) Submit a question References (44)

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Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
  • Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
  • Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
  • Immunoprecipitation - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
  • Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
  • Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
  • Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
  • Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
  • Anti-Integrin alpha V antibody [EPR16800] (ab179475)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16800] to Integrin alpha V
  • Suitable for: WB, IP, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free HRP PE

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Recombinant Human Integrin alpha V protein (Tagged) (ab114240)
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View more associated products

Overview

  • Product name

    Anti-Integrin alpha V antibody [EPR16800]
    See all Integrin alpha V primary antibodies
  • Description

    Rabbit monoclonal [EPR16800] to Integrin alpha V
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HUVEC, HT-29, A549, C6 and NIH/3T3 whole cell lysates; Human fetal kidney and fetal brain lysates; Mouse brain, kidney and spleen lysates; Rat brain and kidney lysates. IHC: Human kidney, human transitional cell carcinoma of bladder and mouse kidney tissues. ICC/IF: A549 cells. IP: A549 whole cell extract.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16800
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Integrins
    • Cardiovascular
    • Blood
    • Platelets
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Leukocyte recruitment
    • Integrins
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-Integrin alpha V antibody [EPR16800] (ab204684)
    • HRP Anti-Integrin alpha V antibody [EPR16800] (ab205474)
    • Alexa Fluor® 594 Anti-Integrin alpha V antibody [EPR16800] (ab207286)
    • PE Anti-Integrin alpha V antibody [EPR16800] (ab211845)
    • Anti-Integrin alpha V antibody [EPR16800] - Low endotoxin, Azide free (ab222222)
    • Anti-Integrin alpha V antibody [EPR16800] - BSA and Azide free (ab271932)
    • Alexa Fluor® 555 Anti-Integrin alpha V antibody [EPR16800] (ab275351)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Integrin alpha V protein (Tagged) (ab114240)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (4)
1/5000. Detects a band of approximately 125, 135 kDa (predicted molecular weight: 116 kDa).
IP
1/40.
ICC/IF (3)
1/500.
IHC-P (3)
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/5000. Detects a band of approximately 125, 135 kDa (predicted molecular weight: 116 kDa).
IP
1/40.
ICC/IF
1/500.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P06756 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3685 Human
    • Entrez Gene: 16410 Mouse
    • Entrez Gene: 296456 Rat
    • Omim: 193210 Human
    • SwissProt: P06756 Human
    • SwissProt: P43406 Mouse
    • Unigene: 436873 Human
    • Unigene: 227 Mouse
    • Alternative names

      • antigen identified by monoclonal antibody L230 antibody
      • CD 51 antibody
      • CD51 antibody
      • DKFZp686A08142 antibody
      • Integrin alpha five antibody
      • integrin alpha V beta 3 antibody
      • Integrin alpha-5 antibody
      • integrin alpha-V antibody
      • Integrin alpha-V light chain antibody
      • integrin alphaVbeta3 antibody
      • integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) antibody
      • ITAV_HUMAN antibody
      • ITGAV antibody
      • MSK 8 antibody
      • Msk8 antibody
      • Vitronectin receptor subunit alpha antibody
      • VNRA antibody
      • VTNR antibody
      see all

    Images

    • Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
      Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
      All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/1000 dilution

      Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate. with NFDM/TBST
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate treated with PNGase F. with NFDM/TBST

      Lysates/proteins at 15 µg per lane.

      Blocking peptides at 5 % per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated.)

      Predicted band size: 116 kDa
      Observed band size: 116.135 kDa why is the actual band size different from the predicted?


      Exposure time: 20 seconds
    • Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
      Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
      All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/1000 dilution

      Lane 1 : Untreated K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate. with NFDM/TBST
      Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) treated with PNGase F whole cell lysate. with NFDM/TBST

      Lysates/proteins at 15 µg per lane.

      Blocking peptides at 5 % per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated.)

      Predicted band size: 116 kDa
      Observed band size: 116.135 kDa why is the actual band size different from the predicted?


      Exposure time: 80 seconds
    • Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
      Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
      All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/50000 dilution

      Lane 1 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysates
      Lane 2 : HT-29 (Human colorectal adenocarcinoma cells) whole cell lysates
      Lane 3 : A549 (Human lung carcinoma) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 116 kDa
      Observed band size: 125 kDa why is the actual band size different from the predicted?



      Blocking/dilution buffer: 5% NFDM/TBST.

      ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

      Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary antibody at 1/500 dilution.

      Membrane and weak cytoplasm staining on human transitional cell carcinoma of bladder is observed. Counterstained with hematoxylin.

      Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
      Immunoprecipitation - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

      Integrin alpha V was immunoprecipitated from 1 mg of A549 (Human lung carcinoma) whole cell extract with ab179475 at 1/40 dilution. Western blot analysis was performed from the immunoprecipitate using ab179475 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

      Lane 1: A549 whole cell extract.

      Lane 2: PBS instead of A549 whole cell extract.


      Blocking/Dilution buffer: 5% NFDM/TBST.

      ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.

    • Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
      Immunocytochemistry/ Immunofluorescence - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

      Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma) cells labeling Integrin alpha V with ab179475 at 1/500 dilution, followed by Goat anti-rabbit Alexa Fluor® 488 (IgG) (ab150077) secondary antibody at 1/400 dilution (green).

      Confocal image showing membrane and cytoplasm staining on A549 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse AlexaFluor®594 secondary antibody) at 1/500 dilution (red).

      The negative controls are as follows:

      -ve control 1: ab179475 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.

      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

    • Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
      Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
      All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/5000 dilution

      Lane 1 : Human fetal kidney lysates
      Lane 2 : Human fetal brain lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 116 kDa
      Observed band size: 125 kDa why is the actual band size different from the predicted?



      Blocking/dilution buffer: 5% NFDM/TBST.

      ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

      Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary antibody at 1/500 dilution.

      Membrane and cytoplasm staining on human kidney tubules is observed. Counterstained with hematoxylin.

      Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
      Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
      All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/5000 dilution

      Lane 1 : Mouse brain lysates
      Lane 2 : Mouse kidney lysates
      Lane 3 : Mouse spleen lysates
      Lane 4 : Rat brain lysates
      Lane 5 : Rat kidney lysates
      Lane 6 : C6 (Rat glial tumor cells) whole cell lysates
      Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates
      Lane 8 : Raji (Human Burkitt's lymphoma cell line) whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 116 kDa
      Observed band size: 125,135 kDa why is the actual band size different from the predicted?



      Blocking/dilution buffer: 5% NFDM/TBST.

      ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR16800] (ab179475)

      Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary at 1/500 dilution.

      Membrane and cytoplasm staining on mouse kidney tubule and glomerulus is observed. Counterstained with hematoxylin.

      Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)
      Western blot - Anti-Integrin alpha V antibody [EPR16800] (ab179475)This image is courtesy of an anonymous Abreview.
      All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/2000 dilution (8 hours at 4°C)

      All lanes : Mouse skin whole cell lysate

      Lysates/proteins at 40 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG(H+L)-HRP at 1/5000 dilution

      Developed using the ECL technique.

      Predicted band size: 116 kDa


      Exposure time: 25 seconds


      Diluent: 2% BSA in 1X TBST.

      Blocked using 5% BSA for 1 hour at RT.

       

      See Abreview

    • Anti-Integrin alpha V antibody [EPR16800] (ab179475)
      Anti-Integrin alpha V antibody [EPR16800] (ab179475)

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (44)

    Publishing research using ab179475? Please let us know so that we can cite the reference in this datasheet.

    ab179475 has been referenced in 44 publications.

    • Sui S & Hou Y Assessing Doxorubicin-Induced Cardiomyopathy by 99mTc-3PRGD2 Scintigraphy Targeting Integrin avß3 in a Rat Model. Nuklearmedizin 60:289-298 (2021). PubMed: 33638136
    • Xu R  et al. Secreted frizzled-related protein 3 was genetically and functionally associated with developmental dysplasia of the hip. Aging (Albany NY) 13:11281-11295 (2021). PubMed: 33820867
    • Deng C  et al. Targeted apoptosis of macrophages and osteoclasts in arthritic joints is effective against advanced inflammatory arthritis. Nat Commun 12:2174 (2021). PubMed: 33846342
    • Schiesser JV  et al. Integrin avß5 heterodimer is a specific marker of human pancreatic beta cells. Sci Rep 11:8315 (2021). PubMed: 33859325
    • Klingler F  et al. Unveiling the CHO surfaceome: Identification of cell surface proteins reveals cell aggregation-relevant mechanisms. Biotechnol Bioeng 118:3015-3028 (2021). PubMed: 33951178
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 10 Abreviews or Q&A

    Western blot abreview for Anti-Integrin alpha V antibody [EPR16800]

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (heart)
    Gel Running Conditions
    Reduced Denaturing (4-12)
    Loading amount
    10 µg
    Specification
    heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 17 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha V antibody [EPR16800]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human prostate tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA buffer (pH 9.0)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Human prostate tissue
    Blocking step
    Blocking according to ab210061 - Double stain IHC kit plus goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
    Fixative
    No fixation
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    Submitted Aug 11 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha V antibody [EPR16800]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Mice prostate tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA buffer (pH 9.0)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Mice prostate tissue
    Blocking step
    Blocking according to ab210061 - Double stain IHC kit plus goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
    Fixative
    No fixation
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    Submitted Aug 11 2021

    Western blot abreview for Anti-Integrin alpha V antibody [EPR16800]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Rat hepatocytes)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    35 µg
    Specification
    Rat hepatocytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    Submitted Aug 11 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Integrin alpha V antibody [EPR16800]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (RM-1 - mouse prostate cancer cells)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    RM-1 - mouse prostate cancer cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    Submitted Dec 03 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Integrin alpha V antibody [EPR16800]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Rat hepatocytes)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    Rat hepatocytes
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    Submitted Sep 10 2020

    Western blot abreview for Anti-Integrin alpha V antibody [EPR16800]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (22Rv1 - prostate cancer cells)
    Gel Running Conditions
    Reduced Denaturing (8% SDS-PAGE)
    Loading amount
    25 µg
    Specification
    22Rv1 - prostate cancer cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    Submitted Aug 31 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Integrin alpha V antibody [EPR16800]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (PC-3 - prostate cancer cells)
    Permeabilization
    Yes - 0.2% Triton X-100 in PBS
    Specification
    PC-3 - prostate cancer cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Armen Petrosyan

    Verified customer

    Submitted Aug 28 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Integrin alpha V antibody [EPR16800]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human skin SCC)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer
    Permeabilization
    No
    Specification
    Human skin SCC
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Yong Sun Lee

    Verified customer

    Submitted Jun 27 2018

    Western blot abreview for Anti-Integrin alpha V antibody [EPR16800]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse skin)
    Gel Running Conditions
    Reduced Denaturing (8%)
    Loading amount
    40 µg
    Specification
    Mouse skin
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 20 2018

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