Recombinant Anti-Integrin alpha V antibody [EPR19669] (ab208012)
![Western blot - Anti-Integrin alpha V antibody [EPR19669] (ab208012)](https://www.abcam.com/ps/products/208/ab208012/Images/ab208012-261174-anti-integrin-alpha-v-antibody-epr19669-western-blot.jpg "Western blot - Anti-Integrin alpha V antibody [EPR19669] (ab208012)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19669] to Integrin alpha V
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Integrin alpha V antibody [EPR19669]
See all Integrin alpha V primary antibodies -
Description
Rabbit monoclonal [EPR19669] to Integrin alpha V -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A549, A431, HT-29, HUVEC, C6, PC-12, NIH/3T3 and RAW 264.7 whole cell lysates; human fetal brain, fetal heart and fetal kidney lysates; mouse brain, kidney and spleen lysates; rat kidney and spleen lysates. IHC-P: Human kidney and bladder cancer tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19669 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab208012 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC is recommended for human only. |
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| WB |
1/2000. Detects a band of approximately 130-140 kDa (predicted molecular weight: 87 kDa).
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| Notes |
|---|
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IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC is recommended for human only. |
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WB
1/2000. Detects a band of approximately 130-140 kDa (predicted molecular weight: 87 kDa). |
Target
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Function
The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3685 Human
- Entrez Gene: 16410 Mouse
- Entrez Gene: 296456 Rat
- Omim: 193210 Human
- SwissProt: P06756 Human
- SwissProt: P43406 Mouse
- Unigene: 436873 Human
- Unigene: 227 Mouse
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Alternative names
- antigen identified by monoclonal antibody L230 antibody
- CD 51 antibody
- CD51 antibody
see all
Images
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Western blot - Anti-Integrin alpha V antibody [EPR19669] (ab208012)All lanes : Anti-Integrin alpha V antibody [EPR19669] (ab208012) at 1/2000 dilution
Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lane 4 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 87 kDa
Observed band size: 130-140 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 22740495)
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![Western blot - Anti-Integrin alpha V antibody [EPR19669] (ab208012)](https://www.abcam.com/ps/products/208/ab208012/Images/ab208012-261173-anti-integrin-alpha-v-antibody-epr19669-western-blot.jpg)
Western blot - Anti-Integrin alpha V antibody [EPR19669] (ab208012)All lanes : Anti-Integrin alpha V antibody [EPR19669] (ab208012) at 1/2000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human fetal heart tissue lysate
Lane 3 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 87 kDa
Observed band size: 130-140 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Integrin alpha V antibody [EPR19669] (ab208012)](https://www.abcam.com/ps/products/208/ab208012/Images/ab208012-261172-anti-integrin-alpha-v-antibody-epr19669-western-blot.jpg)
Western blot - Anti-Integrin alpha V antibody [EPR19669] (ab208012)All lanes : Anti-Integrin alpha V antibody [EPR19669] (ab208012) at 1/2000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 5 : Mouse brain tissue lysate
Lane 6 : Mouse kidney tissue lysate
Lane 7 : Mouse spleen tissue lysate
Lane 8 : Rat kidney tissue lysate
Lane 9 : Rat spleen tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 100000 µg
Predicted band size: 87 kDa
Observed band size: 130-140 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-3: 5 seconds; Lane 4/9: 30 seconds; Lane 5-7: 1 minute; Lane 8: 15 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR19669] (ab208012)](https://www.abcam.com/ps/products/208/ab208012/Images/ab208012-261171-anti-integrin-alpha-v-antibody-epr19669-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR19669] (ab208012)Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling CD51 with ab208012 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic and membrane staining on human kidney is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR19669] (ab208012)](https://www.abcam.com/ps/products/208/ab208012/Images/ab208012-261170-anti-integrin-alpha-v-antibody-epr19669-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha V antibody [EPR19669] (ab208012)Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling CD51 with ab208012 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Membrane staining on human bladder cancer is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Anti-Integrin alpha V antibody [EPR19669] (ab208012)](https://www.abcam.com/ps/products/208/ab208012/Images/ab208012-6-benefits-of-recombinant-antibodies.png)
Anti-Integrin alpha V antibody [EPR19669] (ab208012)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab208012 has been referenced in 2 publications.
- Endo Y et al. T-DM1-resistant cells gain high invasive activity via EGFR and integrin cooperated pathways. MAbs 10:1003-1017 (2018). PubMed: 30130447
- Pan F et al. Dendritic Cells from Rheumatoid Arthritis Patient Peripheral Blood Induce Th17 Cell Differentiation via miR-363/Integrin av/TGF-ß Axis. Scand J Immunol 85:441-449 (2017). PubMed: 28376277