Recombinant Anti-Integrin alpha V antibody [EPR5583] (ab124968)
![Western blot - Anti-Integrin alpha V antibody [EPR5583] (ab124968)](https://www.abcam.com/ps/products/124/ab124968/Images/ab124968-468737-anti-integrin-alpha-v-antibody-epr5583-westernblot-human-mouse-rat.jpg "Western blot - Anti-Integrin alpha V antibody [EPR5583] (ab124968)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5583] to Integrin alpha V
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Integrin alpha V antibody [EPR5583]
See all Integrin alpha V primary antibodies -
Description
Rabbit monoclonal [EPR5583] to Integrin alpha V -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Integrin alpha V aa 1000-1100 (intracellular). The exact sequence is proprietary.
Database link: P06756 -
Positive control
- A549, A431, C6, RAW264.7, PC12, and NIH 3T3 cell lysates; A549 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Dissociation constant (KD)
KD = 3.20 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5583 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 25, 150 kDa (predicted molecular weight: 86, 116 kDa).
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| Notes |
|---|
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 25, 150 kDa (predicted molecular weight: 86, 116 kDa). |
Target
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Function
The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3685 Human
- Entrez Gene: 16410 Mouse
- Entrez Gene: 296456 Rat
- Omim: 193210 Human
- SwissProt: P06756 Human
- SwissProt: P43406 Mouse
- Unigene: 436873 Human
- Unigene: 227 Mouse
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Alternative names
- antigen identified by monoclonal antibody L230 antibody
- CD 51 antibody
- CD51 antibody
see all
Images
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Western blot - Anti-Integrin alpha V antibody [EPR5583] (ab124968)All lanes : Anti-Integrin alpha V antibody [EPR5583] (ab124968) at 1/1000 dilution (Purified)
Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 3 : HUVEC (Human umbilical vein endothelial cell) whole cell lysate
Lane 4 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 86, 116 kDa
Observed band size: 150, 27-30 kDa why is the actual band size different from the predicted?The bands detected are consistent with what have been described in the literatures (PMID: 11741954, 27033701). Full-length Integrin alpha V: 150kDa; Integrin alpha V light chain: 27-30kDa
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![Anti-Integrin alpha V antibody [EPR5583] (ab124968)](https://www.abcam.com/ps/products/124/ab124968/Images/ab124968-4-benefits-of-recombinant-antibodies.png)
Anti-Integrin alpha V antibody [EPR5583] (ab124968)
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab124968 has been referenced in 7 publications.
- Nguyen SL et al. Integrins mediate placental extracellular vesicle trafficking to lung and liver in vivo. Sci Rep 11:4217 (2021). PubMed: 33602965
- Aseervatham J & Ogbureke KUE Effects of DSPP and MMP20 Silencing on Adhesion, Metastasis, Angiogenesis, and Epithelial-Mesenchymal Transition Proteins in Oral Squamous Cell Carcinoma Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32630820
- Kamarajan P et al. Periodontal pathogens promote cancer aggressivity via TLR/MyD88 triggered activation of Integrin/FAK signaling that is therapeutically reversible by a probiotic bacteriocin. PLoS Pathog 16:e1008881 (2020). PubMed: 33002094
- Mygind KJ et al. Loss of ADAM9 expression impairs ß1 integrin endocytosis, focal adhesion formation and cancer cell migration. J Cell Sci 131:N/A (2018). PubMed: 29142101
- Barr S et al. Development, fabrication and evaluation of a novel biomimetic human breast tissue derived breast implant surface. Acta Biomater 49:260-271 (2017). PubMed: 27919840
- Winkler J et al. Cellular apoptosis susceptibility (CAS) is linked to integrin ß1 and required for tumor cell migration and invasion in hepatocellular carcinoma (HCC). Oncotarget 7:22883-92 (2016). WB . PubMed: 27015362
- Zhang C et al. Molecular magnetic resonance imaging of activated hepatic stellate cells with ultrasmall superparamagnetic iron oxide targeting integrin avß3 for staging liver fibrosis in rat model. Int J Nanomedicine 11:1097-108 (2016). WB . PubMed: 27051285