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    products/primary-antibodies/integrin-linked-ilk-antibody-epr1592-ab76468.pdf

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Signal Transduction Protein Phosphorylation Tyrosine Kinases Other
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Integrin linked ILK antibody [EPR1592] (ab76468)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (13)

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Western blot - Anti-Integrin linked ILK antibody [EPR1592] (ab76468)
  • Immunocytochemistry/ Immunofluorescence - Anti-Integrin linked ILK antibody [EPR1592] (ab76468)
  • Western blot - Anti-Integrin linked ILK antibody [EPR1592] (ab76468)
  • Immunocytochemistry/ Immunofluorescence - Anti-Integrin linked ILK antibody [EPR1592] (ab76468)
  • Immunocytochemistry/ Immunofluorescence - Anti-Integrin linked ILK antibody [EPR1592] (ab76468)
  • Flow Cytometry (Intracellular) - Anti-Integrin linked ILK antibody [EPR1592] (ab76468)
  • Anti-Integrin linked ILK antibody [EPR1592] (ab76468)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1592] to Integrin linked ILK
  • Suitable for: WB, ICC/IF, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-Integrin linked ILK antibody [EPR1592]
    See all Integrin linked ILK primary antibodies
  • Description

    Rabbit monoclonal [EPR1592] to Integrin linked ILK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt (Intra)more details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human Integrin linked ILK. The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, A431 and Jurkat whole cell lysate (ab7899); HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1592
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other

Associated products

  • Alternative Versions

    • PE Anti-Integrin linked ILK antibody [EPR1592] (ab211694)
    • Anti-Integrin linked ILK antibody [EPR1592] - BSA and Azide free (ab239884)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Jurkat cytoplasmic extract lysate (ab14842)
  • Recombinant Protein

    • Recombinant Human Integrin linked ILK protein (ab83119)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/5000 - 1/50000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
ICC/IF
1/500.

For unpurified use at 1/100 - 1/250

Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Notes
WB
1/5000 - 1/50000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
ICC/IF
1/500.

For unpurified use at 1/100 - 1/250

Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Application notes
Is unsuitable for IHC-P or IP.

Target

  • Function

    Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
  • Tissue specificity

    Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver.
  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 5 ANK repeats.
    Contains 1 protein kinase domain.
  • Domain

    A PH-like domain is involved in phosphatidylinositol phosphate binding.
  • Post-translational
    modifications

    Autophosphorylated on serine residues.
  • Cellular localization

    Cell junction > focal adhesion. Cell membrane.
  • Target information above from: UniProt accession Q13418 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3611 Human
    • Entrez Gene: 16202 Mouse
    • Entrez Gene: 170922 Rat
    • Omim: 602366 Human
    • SwissProt: Q13418 Human
    • SwissProt: O55222 Mouse
    • SwissProt: Q99J82 Rat
    • Unigene: 5158 Human
    • Unigene: 706355 Human
    • Unigene: 274846 Mouse
    • Unigene: 95042 Rat
    see all
  • Alternative names

    • 59 kDa serine/threonine protein kinase antibody
    • 59 kDa serine/threonine-protein kinase antibody
    • DKFZp686F1765 antibody
    • Epididymis secretory protein Li 28 antibody
    • HEL S 28 antibody
    • ILK 1 antibody
    • ILK 2 antibody
    • ILK antibody
    • ILK-1 antibody
    • ILK-2 antibody
    • ILK_HUMAN antibody
    • ILK1 antibody
    • ILK2 antibody
    • Integrin linked kinase 2 antibody
    • Integrin linked Kinase antibody
    • Integrin linked protein kinase antibody
    • Integrin-linked protein kinase antibody
    • p59 antibody
    • p59ILK antibody
    see all

Images

  • Western blot - Anti-Integrin linked ILK antibody [EPR1592] (ab76468)
    Western blot - Anti-Integrin linked ILK antibody [EPR1592] (ab76468)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Integrin linked ILK knockout HAP1 cell lysate (20 µg)
    Lane 3: Hek293 cell lysate (20 µg)
    Lane 4: K562 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab76468 observed at 51 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab76468 was shown to specifically react with Integrin linked ILK when Integrin linked ILK knockout samples were used. Wild-type and Integrin linked ILK knockout samples were subjected to SDS-PAGE. ab76468 and ab8245 (loading control to GAPDH) were diluted at 1/5000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Integrin linked ILK antibody [EPR1592] (ab76468)
    Immunocytochemistry/ Immunofluorescence - Anti-Integrin linked ILK antibody [EPR1592] (ab76468)

    Immunocytochemistry/Immunofluorescence analysis of A431 (human epidermoid carcinoma) cells labelling Integrin linked ILK (green) with purified ab76468 at 1/500. Cells were fixed with 100% methanol. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were stained blue with DAPI.  

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • Western blot - Anti-Integrin linked ILK antibody [EPR1592] (ab76468)
    Western blot - Anti-Integrin linked ILK antibody [EPR1592] (ab76468)
    All lanes : Anti-Integrin linked ILK antibody [EPR1592] (ab76468) at 1/50000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Integrin linked ILK antibody [EPR1592] (ab76468)
    Immunocytochemistry/ Immunofluorescence - Anti-Integrin linked ILK antibody [EPR1592] (ab76468)
    ab76468 at 1/100 dilution staining Integrin linked ILK in HeLa cells by Immunofluorescence.
  • Immunocytochemistry/ Immunofluorescence - Anti-Integrin linked ILK antibody [EPR1592] (ab76468)
    Immunocytochemistry/ Immunofluorescence - Anti-Integrin linked ILK antibody [EPR1592] (ab76468)
    ab76468 at 1/100 dilution staining Integrin linked ILK in HeLa cells by Immunofluorescence.
  • Flow Cytometry (Intracellular) - Anti-Integrin linked ILK antibody [EPR1592] (ab76468)
    Flow Cytometry (Intracellular) - Anti-Integrin linked ILK antibody [EPR1592] (ab76468)

    Overlay histogram showing HEK293 cells stained with ab76468 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76468, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit DyLight® 488 (IgG; H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. 

     

     

  • Anti-Integrin linked ILK antibody [EPR1592] (ab76468)
    Anti-Integrin linked ILK antibody [EPR1592] (ab76468)

Protocols

  • Flow cytometry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (13)

Publishing research using ab76468? Please let us know so that we can cite the reference in this datasheet.

ab76468 has been referenced in 13 publications.

  • Huang LL  et al. Integrin-Linked Kinase (ILK) Regulates Urinary Stem Cells Differentiation into Smooth Muscle via NF-?B Signal Pathway. Stem Cells Int 2021:6633111 (2021). PubMed: 33854551
  • Morigi M  et al. C3a receptor blockade protects podocytes from injury in diabetic nephropathy. JCI Insight 5:N/A (2020). PubMed: 32161193
  • Garcia-Keller C  et al. Extracellular Matrix Signaling Through ß3 Integrin Mediates Cocaine Cue-Induced Transient Synaptic Plasticity and Relapse. Biol Psychiatry 86:377-387 (2019). PubMed: 31126696
  • Jacquemet G  et al. Filopodome Mapping Identifies p130Cas as a Mechanosensitive Regulator of Filopodia Stability. Curr Biol 29:202-216.e7 (2019). PubMed: 30639111
  • Choe SR  et al. RCP induces FAK phosphorylation and ovarian cancer cell invasion with inhibition by curcumin. Exp Mol Med 50:52 (2018). WB . PubMed: 29700289
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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Western blot abreview for Anti-Integrin linked ILK antibody [EPR1592]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (platelet)
Loading amount
25 µg
Specification
platelet
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Submitted Jan 04 2012

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