Anti-Interferon alpha 2 antibody (ab193055)
Key features and details
- Rabbit polyclonal to Interferon alpha 2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Interferon alpha 2 antibody
See all Interferon alpha 2 primary antibodies -
Description
Rabbit polyclonal to Interferon alpha 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human Interferon alpha 2 aa 1 to the C-terminus.
Database link: P01563 -
Positive control
- WB: Mouse liver tissue lysate, stomach tissue. IHC-P: Human kidney tissue, lung tissue, glioma tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 49% PBS, 50% Glycerol, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
>95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/5000. Predicted molecular weight: 22 kDa.
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| IHC-P | (1) |
1/20 - 1/200.
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| ICC/IF |
1/50 - 1/200.
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| Notes |
|---|
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WB
1/500 - 1/5000. Predicted molecular weight: 22 kDa. |
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IHC-P
1/20 - 1/200. |
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ICC/IF
1/50 - 1/200. |
Target
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Function
Produced by macrophages, IFN-alpha have antiviral activities. -
Sequence similarities
Belongs to the alpha/beta interferon family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3440 Human
- Entrez Gene: 15965 Mouse
- Omim: 147562 Human
- SwissProt: P01563 Human
- SwissProt: P01573 Mouse
- Unigene: 211575 Human
- Unigene: 14091 Mouse
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Alternative names
- Alpha 2a interferon antibody
- IFN alpha 2 antibody
- IFN alphaA antibody
see all
Images
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Western blot - Anti-Interferon alpha 2 antibody (ab193055)Anti-Interferon alpha 2 antibody (ab193055) at 2 µg/ml + Mouse liver tissue lysate
Secondary
Polyclonal goat anti-rabbit at 1/10000 dilution
Predicted band size: 22 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon alpha 2 antibody (ab193055)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Interferon alpha 2 with ab193055 at a dilution of 1/100.
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Immunocytochemistry/ Immunofluorescence - Anti-Interferon alpha 2 antibody (ab193055)Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cells from cervix adenocarcinoma cell line) cells labeling Interferon alpha 2 with ab193055 at 1/100 dilution. The nuclear counterstain is DAPI (blue).
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Western blot - Anti-Interferon alpha 2 antibody (ab193055)Anti-Interferon alpha 2 antibody (ab193055) at 3 µg/ml + Mouse stomach tissue
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon alpha 2 antibody (ab193055)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labeling Interferon alpha 2 with ab193055 at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon alpha 2 antibody (ab193055)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue labelling Interferon alpha 2 with ab193055 at a dilution of 1/100.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab193055 has been referenced in 6 publications.
- Xia P et al. NLRP3 inflammasome up-regulates major histocompatibility complex class I expression and promotes inflammatory infiltration in polymyositis. BMC Immunol 23:39 (2022). PubMed: 35965334
- Urabe A et al. Klotho deficiency intensifies hypoxia-induced expression of IFN-α/β through upregulation of RIG-I in kidneys. PLoS One 16:e0258856 (2021). PubMed: 34673800
- Zhan X et al. Glioma stem-like cells evade interferon suppression through MBD3/NuRD complex-mediated STAT1 downregulation. J Exp Med 217:N/A (2020). PubMed: 32181805
- Liu X et al. Overexpression of microRNA-216a-3p Accelerates the Inflammatory Response in Cardiomyocytes in Type 2 Diabetes Mellitus by Targeting IFN-a2. Front Endocrinol (Lausanne) 11:522340 (2020). PubMed: 33329376
- Zhang C et al. Mycobacterium abscessus-Bronchial Epithelial Cells Cross-Talk Through Type I Interferon Signaling. Front Immunol 10:2888 (2019). PubMed: 31921151
- Marcial GE et al. Lactobacillus johnsonii N6.2 Modulates the Host Immune Responses: A Double-Blind, Randomized Trial in Healthy Adults. Front Immunol 8:655 (2017). PubMed: 28659913