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  1. Link

    products/primary-antibodies/interferon-alpha-2-antibody-ab193055.pdf

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Immunology Innate Immunity Cytokines Interferons
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Anti-Interferon alpha 2 antibody (ab193055)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (4)

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Western blot - Anti-Interferon alpha 2 antibody (ab193055)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon alpha 2 antibody (ab193055)
  • Immunocytochemistry/ Immunofluorescence - Anti-Interferon alpha 2 antibody (ab193055)
  • Western blot - Anti-Interferon alpha 2 antibody (ab193055)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon alpha 2 antibody (ab193055)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon alpha 2 antibody (ab193055)

Key features and details

  • Rabbit polyclonal to Interferon alpha 2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Interferon alpha 2 antibody [EPR19074] (ab196221)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Interferon alpha 2 antibody
    See all Interferon alpha 2 primary antibodies
  • Description

    Rabbit polyclonal to Interferon alpha 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Interferon alpha 2 aa 1 to the C-terminus.
    Database link: P01563

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Mouse liver tissue lysate, stomach tissue. IHC-P: Human kidney tissue, lung tissue, glioma tissue. ICC/IF: HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituents: 49% PBS, 50% Glycerol, 0.03% Proclin 300
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    >95%
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • Interferons
    • Cancer
    • Tumor immunology
    • Cytokines
    • Interferons
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Interferon alpha 2 protein (Active) (ab200262)
  • Related Products

    • Recombinant human Interferon alpha 2 protein (ab151900)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab193055 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/5000. Predicted molecular weight: 22 kDa.
IHC-P (1)
1/20 - 1/200.
ICC/IF
1/50 - 1/200.
Notes
WB
1/500 - 1/5000. Predicted molecular weight: 22 kDa.
IHC-P
1/20 - 1/200.
ICC/IF
1/50 - 1/200.

Target

  • Function

    Produced by macrophages, IFN-alpha have antiviral activities.
  • Sequence similarities

    Belongs to the alpha/beta interferon family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P01563 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3440 Human
    • Entrez Gene: 15965 Mouse
    • Omim: 147562 Human
    • SwissProt: P01563 Human
    • SwissProt: P01573 Mouse
    • Unigene: 211575 Human
    • Unigene: 14091 Mouse
    • Alternative names

      • Alpha 2a interferon antibody
      • IFN alpha 2 antibody
      • IFN alphaA antibody
      • IFN antibody
      • IFN-alpha-2 antibody
      • IFNA antibody
      • Ifna2 antibody
      • IFNA2_HUMAN antibody
      • IFNA2B antibody
      • Interferon alpha 2a antibody
      • Interferon alpha 2b antibody
      • Interferon alpha A antibody
      • Interferon alpha-2 antibody
      • Interferon alpha-A antibody
      • Interferon antibody
      • LeIF A antibody
      • LeIFA antibody
      • MGC125764 antibody
      • MGC125765 antibody
      see all

    Images

    • Western blot - Anti-Interferon alpha 2 antibody (ab193055)
      Western blot - Anti-Interferon alpha 2 antibody (ab193055)
      Anti-Interferon alpha 2 antibody (ab193055) at 2 µg/ml + Mouse liver tissue lysate

      Secondary
      Polyclonal goat anti-rabbit at 1/10000 dilution

      Predicted band size: 22 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon alpha 2 antibody (ab193055)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon alpha 2 antibody (ab193055)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Interferon alpha 2 with ab193055 at a dilution of 1/100.

    • Immunocytochemistry/ Immunofluorescence - Anti-Interferon alpha 2 antibody (ab193055)
      Immunocytochemistry/ Immunofluorescence - Anti-Interferon alpha 2 antibody (ab193055)

      Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cells from cervix adenocarcinoma cell line) cells labeling Interferon alpha 2 with ab193055 at 1/100 dilution. The nuclear counterstain is DAPI (blue).

    • Western blot - Anti-Interferon alpha 2 antibody (ab193055)
      Western blot - Anti-Interferon alpha 2 antibody (ab193055)
      Anti-Interferon alpha 2 antibody (ab193055) at 3 µg/ml + Mouse stomach tissue

      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution

      Predicted band size: 22 kDa
      Observed band size: 22 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon alpha 2 antibody (ab193055)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon alpha 2 antibody (ab193055)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labeling Interferon alpha 2 with ab193055 at 1/100 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon alpha 2 antibody (ab193055)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon alpha 2 antibody (ab193055)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue labelling Interferon alpha 2 with ab193055 at a dilution of 1/100.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (4)

    Publishing research using ab193055? Please let us know so that we can cite the reference in this datasheet.

    ab193055 has been referenced in 4 publications.

    • Zhan X  et al. Glioma stem-like cells evade interferon suppression through MBD3/NuRD complex-mediated STAT1 downregulation. J Exp Med 217:N/A (2020). PubMed: 32181805
    • Liu X  et al. Overexpression of microRNA-216a-3p Accelerates the Inflammatory Response in Cardiomyocytes in Type 2 Diabetes Mellitus by Targeting IFN-a2. Front Endocrinol (Lausanne) 11:522340 (2020). PubMed: 33329376
    • Zhang C  et al. Mycobacterium abscessus-Bronchial Epithelial Cells Cross-Talk Through Type I Interferon Signaling. Front Immunol 10:2888 (2019). PubMed: 31921151
    • Marcial GE  et al. Lactobacillus johnsonii N6.2 Modulates the Host Immune Responses: A Double-Blind, Randomized Trial in Healthy Adults. Front Immunol 8:655 (2017). PubMed: 28659913

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Interferon alpha 2 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA buffer pH 9.0
    Permeabilization
    No
    Specification
    skin
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 24 2022

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