Recombinant Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] - BSA and Azide free (ab247992)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6244] to Interferon alpha/beta receptor 1 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] - BSA and Azide free
See all Interferon alpha/beta receptor 1 primary antibodies -
Description
Rabbit monoclonal [EPR6244] to Interferon alpha/beta receptor 1 - BSA and Azide free -
Host species
Rabbit -
Specificity
In mouse and rat tissue lysates this product detects a band in the region of 100 kDa, however we believe this band is non-specific and is not interferon receptor alpha as the immunogen for this antibody shares only 55% homology with the mouse and rat protein. In addition this band also migrates at a lower molecular weight than that detected in human samples.
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Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247992 is the carrier-free version of ab124764.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 2.40 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6244 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab247992 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 90-130 kDa (predicted molecular weight: 64 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 90-130 kDa (predicted molecular weight: 64 kDa). |
Target
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Function
Associates with IFNAR2 to form the type I interferon receptor. Receptor for interferons alpha and beta. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta-subunits themselves. -
Tissue specificity
IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266S. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R. -
Sequence similarities
Belongs to the type II cytokine receptor family.
Contains 3 fibronectin type-III domains. -
Post-translational
modificationsPhosphorylated on tyrosine residues by TYK2 tyrosine kinase. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3454 Human
- Omim: 107450 Human
- SwissProt: P17181 Human
- Unigene: 529400 Human
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Alternative names
- Alpha type antiviral protein antibody
- Antiviral protein, alpha-type antibody
- Antiviral protein, beta-type antibody
see all
Images
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All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : IFNAR1 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 116 kDa why is the actual band size different from the predicted?This data was developed using ab124764, the same antibody clone in a different buffer formulation.
Western blot: Anti-IFNAR1 antibody [EPR6244] (ab124764) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab124764 was shown to bind specifically to IFNAR1. A band was observed at 116 kDa in wild-type HeLa cell lysates with no signal observed at this size in IFNAR1 knockout cell line. To generate this image, wild-type and IFNAR1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit monoclonal [EPR6244] to Interferon alpha/beta receptor 1 (ab124764) at 1/1000 dilution
Predicted band size: 64 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?This data was developed using ab124764, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
In mouse and rat tissue lysates this product detects a band in the region of 100 kDa, however we believe this band is non-specific and is not interferon receptor alpha as the immunogen for this antibody shares only 55% homology with the mouse and rat protein. In addition this band also migrates at a lower molecular weight than that detected in human samples.
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All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (purified)
Lane 1 : HEK-293 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : LNCaP whole cell lysate
Lane 4 : SH-SY5Y whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 90-130 kDa why is the actual band size different from the predicted?This data was developed using ab124764, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (unpurified)
Lane 1 : Mouse brain whole tissue lysate
Lane 2 : Mouse heart whole tissue lysate
Lane 3 : Mouse kidney whole tissue lysate
Lane 4 : Mouse spleen whole tissue lysate
Lane 5 : Rat brain whole tissue lysate
Lane 6 : Rat heart whole cell lysate
Lane 7 : Rat kidney whole cell lysate
Lane 8 : Rat spleen whole tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (H+L), peroxidase conjugated at 1/2000 dilution
Predicted band size: 64 kDa
Exposure time: 3 minutesThis data was developed using ab124764, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
In mouse and rat tissue lysates this product detects a band in the region of 100 kDa, however we believe this band is non-specific and is not interferon receptor alpha as the immunogen for this antibody shares only 55% homology with the mouse and rat protein. In addition this band also migrates at a lower molecular weight than that detected in human samples.
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All lanes : Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (ab124764) at 1/1000 dilution (Unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with IFN-alpha
Lane 3 : K562 cell lysate
Lane 4 : U937 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/200 dilution
Predicted band size: 64 kDaThis data was developed using ab124764, the same antibody clone in a different buffer formulation.
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This data was developed using ab124764, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab247992 has not yet been referenced specifically in any publications.