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RecombinantRabMAb

Recombinant Anti-Interferon gamma antibody [EPR21704] (ab231036)

Reviews (2) Submit a question References (5)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [EPR21704] (ab231036)
  • ELISA - Anti-Interferon gamma antibody [EPR21704] (ab231036)
  • Anti-Interferon gamma antibody [EPR21704] (ab231036)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21704] to Interferon gamma
  • Suitable for: IHC-P, ELISA
  • Reacts with: Human, Recombinant fragment

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Interferon gamma antibody [EPR21704]
    See all Interferon gamma primary antibodies

  • Description

    Rabbit monoclonal [EPR21704] to Interferon gamma

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ELISAmore details

  • Species reactivity

    Reacts with: Human, Recombinant fragment

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Ionomycin and BFA, cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab231036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (2)
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ELISA
Use at an assay dependent concentration.
Notes
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ELISA
Use at an assay dependent concentration.

Target

  • Function

    Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

  • Tissue specificity

    Released primarily from activated T lymphocytes.

  • Involvement in disease

    In Caucasians, genetic variation in IFNG is associated with the risk of aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.

  • Sequence similarities

    Belongs to the type II (or gamma) interferon family.

  • Post-translational
    modifications

    Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • IF 1 antibody
      • IFG antibody
      • IFI antibody
      • ifn g antibody
      • IFN gamma antibody
      • IFN immune antibody
      • IFN, immune antibody
      • IFN-gamma antibody
      • IFNG antibody
      • IFNG_HUMAN antibody
      • Immune interferon antibody
      • Interferon gamma antibody
      • Type II Interferon antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [EPR21704] (ab231036)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Interferon gamma antibody [EPR21704] (ab231036)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Interferon gamma with ab231036 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Sporadic cytoplasmic staining in limphocytes of human tonsil is observed (PMID: 21838712). Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

      Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    • ELISA - Anti-Interferon gamma antibody [EPR21704] (ab231036)
      ELISA - Anti-Interferon gamma antibody [EPR21704] (ab231036)
      ELISA analysis of Human IFN Gamma recombinant protein at 1000 ng/mL with ab231036. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
    • Anti-Interferon gamma antibody [EPR21704] (ab231036)
      Anti-Interferon gamma antibody [EPR21704] (ab231036)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (5)

    Publishing research using ab231036? Please let us know so that we can cite the reference in this datasheet.

    ab231036 has been referenced in 5 publications.

    • Liu J  et al. Prediction and identification of CD4+ T cell epitope for the protective antigens of Mycobacterium tuberculosis. Medicine (Baltimore) 100:e24619 (2021). PubMed: 33578573
    • Watanabe Y  et al. Fecal Stream Diversion Changes Intestinal Environment, Modulates Mucosal Barrier, and Attenuates Inflammatory Cells in Crohn's Disease. Dig Dis Sci N/A:N/A (2021). PubMed: 34041649
    • Cremasco F  et al. Cross-linking of T cell to B cell lymphoma by the T cell bispecific antibody CD20-TCB induces IFN?/CXCL10-dependent peripheral T cell recruitment in humanized murine model. PLoS One 16:e0241091 (2021). PubMed: 33406104
    • Yang Z  et al. ILT4 in Colorectal Cancer Cells Induces Suppressive T Cell Contexture and Disease Progression. Onco Targets Ther 14:4239-4254 (2021). PubMed: 34321889
    • Xu Y  et al. MiRNA-340-5p mediates the functional and infiltrative promotion of tumor-infiltrating CD8+ T lymphocytes in human diffuse large B cell lymphoma. J Exp Clin Cancer Res 39:238 (2020). PubMed: 33168024

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Interferon gamma antibody [EPR21704]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (lymph node)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citric acid pH6, 30 min
    Permeabilization
    No
    Specification
    lymph node
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 14 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Interferon gamma antibody [EPR21704]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Syrian hamster Cell (Brain tissue)
    Permeabilization
    Yes - 1% Triton X-100 in PBS
    Specification
    Brain tissue
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 31 2021

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