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    products/primary-antibodies/ionotropic-glutamate-receptor-2-antibody-epr5032-ab133477.pdf

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Neuroscience Neurotransmitter Amino Acids Glutamate
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RecombinantRabMAb

Recombinant Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (18)

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Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477)
  • Immunocytochemistry/ Immunofluorescence - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477)
  • Flow Cytometry (Intracellular) - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477)
  • Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5032] to Ionotropic Glutamate receptor 2
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

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Overview

  • Product name

    Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032]
    See all Ionotropic Glutamate receptor 2 primary antibodies
  • Description

    Rabbit monoclonal [EPR5032] to Ionotropic Glutamate receptor 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Ionotropic Glutamate receptor 2 aa 150-250. The exact sequence is proprietary.

  • Positive control

    • Flow Cyt (intra): SH-SY5Y cells; ICC/IF: Neuro-2a cells WB: Human hypothalamus and midbrain lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5032
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Amino Acids
    • Glutamate
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Glutamate Receptors
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab199777)
    • Alexa Fluor® 647 Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab199832)
    • Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] - BSA and Azide free (ab240061)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/80.
WB (3)
1/1000 - 1/10000. Predicted molecular weight: 98 kDa.
ICC/IF
1/70.
Notes
Flow Cyt (Intra)
1/80.
WB
1/1000 - 1/10000. Predicted molecular weight: 98 kDa.
ICC/IF
1/70.
Application notes
Is unsuitable for IHC-P.

Target

  • Function

    Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate.
  • Sequence similarities

    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily.
  • Post-translational
    modifications

    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis.
  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression.
  • Target information above from: UniProt accession P42262 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2891 Human
    • Entrez Gene: 14800 Mouse
    • Entrez Gene: 29627 Rat
    • Omim: 138247 Human
    • SwissProt: P42262 Human
    • SwissProt: P23819 Mouse
    • SwissProt: P19491 Rat
    • Unigene: 32763 Human
    • Unigene: 220224 Mouse
    • Unigene: 91361 Rat
    see all
  • Alternative names

    • AMPA 2 antibody
    • AMPA selective glutamate receptor 2 antibody
    • AMPA-selective glutamate receptor 2 antibody
    • AMPA2 antibody
    • GluA2 antibody
    • GLUR 2 antibody
    • GLUR B antibody
    • GluR K2 antibody
    • GluR-2 antibody
    • GluR-B antibody
    • GluR-K2 antibody
    • GLUR2 antibody
    • GLURB antibody
    • Glutamate receptor 2 antibody
    • Glutamate receptor ionotropic AMPA 2 antibody
    • Glutamate receptor ionotropic antibody
    • Gria2 antibody
    • GRIA2_HUMAN antibody
    • HBGR2 antibody
    see all

Images

  • Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477)
    Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477)
    All lanes : Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477) at 1/5000 dilution (Purified)

    Lane 1 : Human hypothalamus lysate
    Lane 2 : Human midbrain lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 98 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477)
    Immunocytochemistry/ Immunofluorescence - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477)

    Immunocytochemistry analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling Ionotropic Glutamate receptor 2 with purified ab133477 at 1/70 dilution (10 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477)
    Flow Cytometry (Intracellular) - Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477)

    Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Ionotropic Glutamate receptor 2 (red) with ab133477 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue). 

     

     

  • Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477)
    Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032] (ab133477)

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (18)

Publishing research using ab133477? Please let us know so that we can cite the reference in this datasheet.

ab133477 has been referenced in 18 publications.

  • Gallina AL  et al. AMPA-Type Glutamate Receptors Associated With Vascular Smooth Muscle Cell Subpopulations in Atherosclerosis and Vascular Injury. Front Cardiovasc Med 8:655869 (2021). PubMed: 33959644
  • Hashem J  et al. Inhibition of 2-Arachidonoylglycerol Metabolism Alleviates Neuropathology and Improves Cognitive Function in a Tau Mouse Model of Alzheimer's Disease. Mol Neurobiol 58:4122-4133 (2021). PubMed: 33939165
  • Liu H  et al. Valproic Acid Induces Autism-Like Synaptic and Behavioral Deficits by Disrupting Histone Acetylation of Prefrontal Cortex ALDH1A1 in Rats. Front Neurosci 15:641284 (2021). PubMed: 33994921
  • Santa-Marinha L  et al. Phospholipase D1 Ablation Disrupts Mouse Longitudinal Hippocampal Axis Organization and Functioning. Cell Rep 30:4197-4208.e6 (2020). PubMed: 32209478
  • Yu L  et al. Butyrate, but not propionate, reverses maternal diet-induced neurocognitive deficits in offspring. Pharmacol Res 160:105082 (2020). PubMed: 32679183
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 09 2020

Western blot abreview for Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Dopaminergic Neurons)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Dopaminergic Neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 20 2020

Western blot abreview for Anti-Ionotropic Glutamate receptor 2 antibody [EPR5032]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Mouse Cell lysate - whole cell (Oligodendrocyte- GluR2 WT and Null)
Specification
Oligodendrocyte- GluR2 WT and Null
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MS. Katherine Given

Verified customer

Submitted Mar 17 2015

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