Recombinant Anti-IP10 antibody [EPR27520-63] (ab308461)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMC (human peripheral blood mononuclear cell) cells labelling IP10 with AB308461 at 1/100 dilution (5.33 ug/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/mL) (Green).

Confocal image showing cytoplasmic staining in subsets of human PBMC treated with INF-gamma (10 ng/mL) for 1 h, then with Brefeldin A (300 ng/mL) for 3 h.

Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling IP10 with AB308461 at 1/100 dilution (5.33 ug/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/mL) (Green).

Confocal image showing cytoplasmic staining in THP-1 cells treated with INF-gamma (200 ng/mL) and lipopolysaccharides (LPS, 50 ng/mL) for 24 h, with addition of brefeldin A (1 ug/mL) for the last 21 h.

Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labelling IP10 with AB308461 at 1/100 dilution (5.33 ug/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/mL) (Green).

Confocal image showing cytoplasmic staining in parental A549 cells treated with INF-gamma (100 ng/mL) and TNF-alpha (10 ng/mL) for 32 h, with addition of brefeldin A (5 ug/mL) for the last 6 h, and no staining in CXCL10 knockout A549 cells with the same condition.

Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized isotype control (Left) / Untreated human PBMC (Middle) / Human PBMC (human peripheral blood mononuclear cell) treated with 10ng/ml IFN-gamma for 1h then treated with 300ng/ml BFA for 3h (Right) cells labelling IP10 with AB308461 at 1/500 dilution (0.1 ug). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were stained with anti-CD14 conjugated to 647. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or ab308461.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (human monocytic leukemia monocyte) treated with INF-gamma (200 ng/mL) and lipopolysaccharides (LPS, 50 ng/mL) for 24 h, with addition of brefeldin A (1 ug/mL) for the last 21 h (Red) / Untreated THP-1 cell (Green) cells labelling IP10 with AB308461 at 1/5000 dilution (0.01 ug)/Red and Green (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.