Recombinant Anti-IP10 antibody [EPR27520-63] (ab308461)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR27520-63] to IP10
- Suitable for: ICC/IF, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IP10 antibody [EPR27520-63]
See all IP10 primary antibodies -
Description
Rabbit monoclonal [EPR27520-63] to IP10 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, Flow Cyt (Intra)more details
Unsuitable for: IHC-P,IP or WB -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: PBMC treated with INF-? (10 ng/mL) for 1h, then with Brefeldin A (300 ng/mL) for 3h, THP-1 cells treated with INF-? (200 ng/mL) and LPS (50 ng/mL) for 24h, brefeldin A (1 µg/mL) for 21 h, A549 treated with INF-? (100 ng/mL) and TNF-a (10 ng/mL) for 32h, brefeldin A (5 µg/mL) for 6h. Flow Cyt (Intra): PBMC treated with 10ng/ml IFN-r for 1h then treated with 300ng/ml BFA for 3h and THP-1 treated with INF-? (200 ng/mL) and LPS (50 ng/mL) for 24h, brefeldin A (1 µg/mL) for 21h.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR27520-63 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab308461 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100.
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|
Flow Cyt (Intra) |
1/500.
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Notes |
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ICC/IF
1/100. |
Flow Cyt (Intra)
1/500. |
Target
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Function
Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsCXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3627 Human
- Omim: 147310 Human
- SwissProt: P02778 Human
- Unigene: 632586 Human
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Alternative names
- Interferon gamma induced factor MOB1, mouse, homolog of antibody
- Interferon gamma induced protein 10 antibody
- 10 kDa interferon gamma induced protein antibody
see all
Images
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized human PBMC (human peripheral blood mononuclear cell) cells labelling IP10 with AB308461 at 1/100 dilution (5.33 ug/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/mL) (Green).
Confocal image showing cytoplasmic staining in subsets of human PBMC treated with INF-gamma (10 ng/mL) for 1 h, then with Brefeldin A (300 ng/mL) for 3 h.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling IP10 with AB308461 at 1/100 dilution (5.33 ug/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/mL) (Green).
Confocal image showing cytoplasmic staining in THP-1 cells treated with INF-gamma (200 ng/mL) and lipopolysaccharides (LPS, 50 ng/mL) for 24 h, with addition of brefeldin A (1 ug/mL) for the last 21 h.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labelling IP10 with AB308461 at 1/100 dilution (5.33 ug/ml), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/mL) (Green).
Confocal image showing cytoplasmic staining in parental A549 cells treated with INF-gamma (100 ng/mL) and TNF-alpha (10 ng/mL) for 32 h, with addition of brefeldin A (5 ug/mL) for the last 6 h, and no staining in CXCL10 knockout A549 cells with the same condition.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (2.5 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 ug/mL).
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Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized isotype control (Left) / Untreated human PBMC (Middle) / Human PBMC (human peripheral blood mononuclear cell) treated with 10ng/ml IFN-gamma for 1h then treated with 300ng/ml BFA for 3h (Right) cells labelling IP10 with AB308461 at 1/500 dilution (0.1 ug). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were stained with anti-CD14 conjugated to 647. Fixed with 2% PFA for 10 min followed by intracellularly staining with rabbit IgG or ab308461.
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Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (human monocytic leukemia monocyte) treated with INF-gamma (200 ng/mL) and lipopolysaccharides (LPS, 50 ng/mL) for 24 h, with addition of brefeldin A (1 ug/mL) for the last 21 h (Red) / Untreated THP-1 cell (Green) cells labelling IP10 with AB308461 at 1/5000 dilution (0.01 ug)/Red and Green (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab308461 has not yet been referenced specifically in any publications.