Recombinant Anti-IP3 receptor antibody [EPR4536] (ab125076)
![Western blot - Anti-IP3 receptor antibody [EPR4536] (ab125076)](https://www.abcam.com/ps/products/125/ab125076/Images/ab125076-267097-anti-ip3-receptor-antibody-epr4536-western-blot.jpg "Western blot - Anti-IP3 receptor antibody [EPR4536] (ab125076)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4536] to IP3 receptor
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IP3 receptor antibody [EPR4536]
See all IP3 receptor primary antibodies -
Description
Rabbit monoclonal [EPR4536] to IP3 receptor -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt (Intra)more details
Unsuitable for: IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human IP3 receptor aa 1-100. The exact sequence is proprietary.
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Positive control
- WB: SH-SY5Y, HeLa and HuT78 cell lysates; IHC-P: Human brain tissue; Flow Cyt (intra): SH-SY5Y cells;
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4536 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125076 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 320 kDa (predicted molecular weight: 314 kDa).
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Flow Cyt (Intra) |
1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 320 kDa (predicted molecular weight: 314 kDa). |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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Flow Cyt (Intra)
1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate. -
Tissue specificity
Widely expressed. -
Involvement in disease
Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15) (SCA15) [MIM:606658]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA15 is an autosomal dominant cerebellar ataxia (ADCA). It is very slow progressing form with a wide range of onset, ranging from childhood to adult. Most patients remain ambulatory. -
Sequence similarities
Belongs to the InsP3 receptor family.
Contains 5 MIR domains. -
Domain
The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region. -
Post-translational
modificationsPhosphorylated by cAMP kinase. Phosphorylation prevents the ligand-induced opening of the calcium channels.
Phosphorylated on tyrosine residues. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 3708 Human
- Omim: 147265 Human
- SwissProt: Q14643 Human
- Unigene: 567295 Human
- Unigene: 715765 Human
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Alternative names
- 4 antibody
- 5-trisphosphate receptor antibody
- 5-trisphosphate receptor type 1 antibody
see all
Images
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Western blot - Anti-IP3 receptor antibody [EPR4536] (ab125076)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: IP3 receptor knockout HAP1 cell lysate (20 µg)
Lane 3: Human brain tissue lysate (20 µg)
Lane 4: SH-SY5Y cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab125076 observed at 270 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab125076 was shown to specifically recognize IP3 receptor in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when IP3 receptor knockout samples were examined. Wild-type and IP3 receptor knockout samples were subjected to SDS-PAGE. ab125076 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4536] (ab125076)](https://www.abcam.com/ps/products/125/ab125076/Images/ab125076-139223-anti-ip3-receptor-antibody-epr4536-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4536] (ab125076)ab125076, at 1/50, staining ITPR1 in paraffin-embedded Human brain tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-IP3 receptor antibody [EPR4536] (ab125076)](https://www.abcam.com/ps/products/125/ab125076/Images/ab125076-5-anti-ip3-receptor-antibody-epr4536-flowcytometry-shsy5y-human.jpg)
Flow Cytometry (Intracellular) - Anti-IP3 receptor antibody [EPR4536] (ab125076)Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling IP3 receptor with purified ab125076 at 1/500 dilution (1 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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![Western blot - Anti-IP3 receptor antibody [EPR4536] (ab125076)](https://www.abcam.com/ps/products/125/ab125076/Images/ab125076-139224-anti-ip3-receptor-antibody-epr4536-western-blot.jpg)
Western blot - Anti-IP3 receptor antibody [EPR4536] (ab125076)All lanes : Anti-IP3 receptor antibody [EPR4536] (ab125076) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HuT78 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 314 kDa -
![Anti-IP3 receptor antibody [EPR4536] (ab125076)](https://www.abcam.com/ps/products/125/ab125076/Images/ab125076-4-benefits-of-recombinant-antibodies.png)
Anti-IP3 receptor antibody [EPR4536] (ab125076)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab125076 has been referenced in 2 publications.
- Rodríguez LR et al. Oxidative stress modulates rearrangement of endoplasmic reticulum-mitochondria contacts and calcium dysregulation in a Friedreich's ataxia model. Redox Biol 37:101762 (2020). PubMed: 33128998
- Gao Y et al. Calcium Receptor and Nitric Oxide Synthase Expression in Circular Muscle of Lower Esophagus from Patients with Achalasia. Chin Med J (Engl) 131:2882-2885 (2018). PubMed: 30511698