Recombinant Anti-IP3 receptor antibody [EPR4537] (ab108517)
![Western blot - Anti-IP3 receptor antibody [EPR4537] (ab108517)](https://www.abcam.com/ps/products/108/ab108517/Images/ab108517-267095-anti-ip3-receptor-antibody-epr4537-western-blot.jpg "Western blot - Anti-IP3 receptor antibody [EPR4537] (ab108517)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4537] to IP3 receptor
- Suitable for: WB, IHC-P, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-IP3 receptor antibody [EPR4537]
See all IP3 receptor primary antibodies -
Description
Rabbit monoclonal [EPR4537] to IP3 receptor -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat brain, SH-SY5Y, Mouse brain, and HeLa lysates IP: Mouse brain cells; IHC-P: Human and Mouse cerebellum tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.10 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4537 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 314 kDa.
|
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| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IP |
1/30.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 314 kDa. |
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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IP
1/30. |
Target
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Function
Intracellular channel that mediates calcium release from the endoplasmic reticulum following stimulation by inositol 1,4,5-trisphosphate. -
Tissue specificity
Widely expressed. -
Involvement in disease
Defects in ITPR1 are the cause of spinocerebellar ataxia type 15 (SCA15) (SCA15) [MIM:606658]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA15 is an autosomal dominant cerebellar ataxia (ADCA). It is very slow progressing form with a wide range of onset, ranging from childhood to adult. Most patients remain ambulatory. -
Sequence similarities
Belongs to the InsP3 receptor family.
Contains 5 MIR domains. -
Domain
The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region. -
Post-translational
modificationsPhosphorylated by cAMP kinase. Phosphorylation prevents the ligand-induced opening of the calcium channels.
Phosphorylated on tyrosine residues. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 3708 Human
- Entrez Gene: 16438 Mouse
- Entrez Gene: 25262 Rat
- Omim: 147265 Human
- SwissProt: Q14643 Human
- SwissProt: P11881 Mouse
- SwissProt: P29994 Rat
- Unigene: 567295 Human
see all -
Alternative names
- 4 antibody
- 5-trisphosphate receptor antibody
- 5-trisphosphate receptor type 1 antibody
see all
Images
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Western blot - Anti-IP3 receptor antibody [EPR4537] (ab108517)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: IP3 receptor knockout HAP1 cell lysate (20 µg)
Lane 3: Human brain tissue lysate (20 µg)
Lane 4: SH-SY5Y cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab108517 observed at 270 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab108517 was shown to specifically recognize IP3 receptor in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when IP3 receptor knockout samples were examined. Wild-type and IP3 receptor knockout samples were subjected to SDS-PAGE. ab108517 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4537] (ab108517)](https://www.abcam.com/ps/products/108/ab108517/Images/ab108517-353499-anti-ip3-receptor-antibody-epr4537-immunohistochemistry-cerebellum-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4537] (ab108517)Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling IP3 with ab108517 at 1/1000 dilution (0.139 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebellum. The section was incubated with ab108517 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4537] (ab108517)](https://www.abcam.com/ps/products/108/ab108517/Images/ab108517-353498-anti-ip3-receptor-antibody-epr4537-immunohistochemistry-cerebellum-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IP3 receptor antibody [EPR4537] (ab108517)Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling IP3 with ab108517 at 1/1000 dilution (0.139 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebellum. The section was incubated with ab108517 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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![Immunoprecipitation - Anti-IP3 receptor antibody [EPR4537] (ab108517)](https://www.abcam.com/ps/products/108/ab108517/Images/ab108517-4-anti-ip3-receptor-antibody-epr4537-immunoprecipitation-brain-mouse.jpg)
Immunoprecipitation - Anti-IP3 receptor antibody [EPR4537] (ab108517)ab108517 (purified) at 1:500 dilution (1.124 µg/ml) immunoprecipitating IP3 receptor in Mouse brain lysate.
Lane 1 (input): Mouse brain lysate 10µg
Lane 2 (+): ab108517 & Mouse brain lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32061 in HeLa whole cell lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM /TBST. -
![Western blot - Anti-IP3 receptor antibody [EPR4537] (ab108517)](https://www.abcam.com/ps/products/108/ab108517/Images/ab108517-121232-anti-ip3-receptor-antibody-epr4537-western-blot.jpg)
Western blot - Anti-IP3 receptor antibody [EPR4537] (ab108517)All lanes : Anti-IP3 receptor antibody [EPR4537] (ab108517) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : SH-SY5Y lysate
Lane 3 : Mouse brain lysate
Lane 4 : HeLa brain lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 314 kDa -
![OI-RD Scanning - Anti-IP3 receptor antibody [EPR4537] (ab108517)](https://www.abcam.com/ps/products/108/ab108517/Images/ab108517-121231-anti-ip3-receptor-antibody-epr4537.jpg)
OI-RD Scanning - Anti-IP3 receptor antibody [EPR4537] (ab108517) -
![Anti-IP3 receptor antibody [EPR4537] (ab108517)](https://www.abcam.com/ps/products/108/ab108517/Images/ab108517-7-benefits-of-recombinant-antibodies.png)
Anti-IP3 receptor antibody [EPR4537] (ab108517)
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab108517 has been referenced in 4 publications.
- Yoneda A et al. Heat shock protein 47 confers chemoresistance on pancreatic cancer cells by interacting with calreticulin and IRE1a. Cancer Sci 112:2803-2820 (2021). PubMed: 34109710
- Huang X et al. Gli1+ Cells Residing in Bone Sutures Respond to Mechanical Force via IP3R to Mediate Osteogenesis. Stem Cells Int 2021:8138374 (2021). PubMed: 34434241
- Bowie E & Goetz SC TTBK2 and primary cilia are essential for the connectivity and survival of cerebellar Purkinje neurons. Elife 9:N/A (2020). PubMed: 31934864
- da Rosa MS et al. 3-Hydroxy-3-Methylglutaric Acid Impairs Redox and Energy Homeostasis, Mitochondrial Dynamics, and Endoplasmic Reticulum-Mitochondria Crosstalk in Rat Brain. Neurotox Res 37:314-325 (2020). PubMed: 31721046