Recombinant Anti-IQGAP1 antibody [EPR5220] (ab133490)
![Western blot - Anti-IQGAP1 antibody [EPR5220] (ab133490)](https://www.abcam.com/ps/products/133/ab133490/Images/ab133490-257909-anti-iqgap1-antibody-epr5220-western-blot.jpg "Western blot - Anti-IQGAP1 antibody [EPR5220] (ab133490)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5220] to IQGAP1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-IQGAP1 antibody [EPR5220]
See all IQGAP1 primary antibodies -
Description
Rabbit monoclonal [EPR5220] to IQGAP1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, 293T, Human placenta, Mouse thymus and F9 cell lysates; Human kidney tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5220 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/10000. Detects a band of approximately 195 kDa (predicted molecular weight: 189 kDa).
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/50 - 1/100.
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| Notes |
|---|
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Flow Cyt (Intra)
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 195 kDa (predicted molecular weight: 189 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/50 - 1/100. |
Target
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Function
Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth. -
Tissue specificity
Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas. -
Sequence similarities
Contains 1 CH (calponin-homology) domain.
Contains 4 IQ domains.
Contains 1 Ras-GAP domain.
Contains 1 WW domain. -
Domain
Regions C1 and C2 can either interact with nucleotide-free CDC42, or interact together, depending on the phosphorylation state of Ser-1443. When Ser-1443 is not phosphorylated, C1 and C2 interact, which prevents binding of nucleotide-free CDC42 and promotes binding of GTP-bound CDC42. Phosphorylation of Ser-1443 prevents interaction between C1 and C2, which opens the structure of the C-terminus and allows binding and sequestration of nucleotide-free CDC42 on both C1 and C2. -
Post-translational
modificationsPhosphorylation of Ser-1443 by PKC prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser-1443 phosphorylation enhances the ability to promote neurite outgrowth. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 8826 Human
- Entrez Gene: 29875 Mouse
- Omim: 603379 Human
- SwissProt: P46940 Human
- SwissProt: Q9JKF1 Mouse
- Unigene: 430551 Human
- Unigene: 207619 Mouse
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Alternative names
- HUMORFA01 antibody
- IQ motif containing GTPase activating protein 1 antibody
- IQGA1_HUMAN antibody
see all
Images
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Western blot - Anti-IQGAP1 antibody [EPR5220] (ab133490)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: IQGAP knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HEK293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133490 observed at 190 kDa. Red - loading control, ab7291, observed at 52 kDa.
ab133490 was shown to specifically react with IQGAP1 when IQGAP1 knockout samples were used. Wild-type and IQGAP1 knockout samples were subjected to SDS-PAGE. ab133490 and ab7291 (loading control to alpha tubulin) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging. -
![Immunocytochemistry/ Immunofluorescence - Anti-IQGAP1 antibody [EPR5220] (ab133490)](https://www.abcam.com/ps/products/133/ab133490/Images/ab133490-273179-anti-iqgap1-antibody-epr5220-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IQGAP1 antibody [EPR5220] (ab133490)Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling IQGAP1 with Purified ab133490 at 1/500 dilution (5 µg/ml). Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP1 antibody [EPR5220] (ab133490)](https://www.abcam.com/ps/products/133/ab133490/Images/ab133490-147146-anti-iqgap1-antibody-epr5220-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP1 antibody [EPR5220] (ab133490)Immunohistochemical analysis of IQGAP1 in paraffin embedded Human kidney tissue, using ab133490 at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-IQGAP1 antibody [EPR5220] (ab133490)](https://www.abcam.com/ps/products/133/ab133490/Images/ab133490-271178-anti-iqgap1-antibody-epr5220-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-IQGAP1 antibody [EPR5220] (ab133490)Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling IQGAP1 with unpurified ab133490 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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![Western blot - Anti-IQGAP1 antibody [EPR5220] (ab133490)](https://www.abcam.com/ps/products/133/ab133490/Images/ab133490-147147-anti-iqgap1-antibody-epr5220-western-blot.jpg)
Western blot - Anti-IQGAP1 antibody [EPR5220] (ab133490)All lanes : Anti-IQGAP1 antibody [EPR5220] (ab133490) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Human placenta lysate
Lane 4 : Mouse thymus lysate
Lane 5 : F9 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 189 kDa
Observed band size: 195 kDa why is the actual band size different from the predicted? -
![Anti-IQGAP1 antibody [EPR5220] (ab133490)](https://www.abcam.com/ps/products/133/ab133490/Images/ab133490-6-benefits-of-recombinant-antibodies.png)
Anti-IQGAP1 antibody [EPR5220] (ab133490)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (12)
ab133490 has been referenced in 12 publications.
- Hoeprich GJ et al. Single-molecule imaging of IQGAP1 regulating actin filament dynamics. Mol Biol Cell 33:ar2 (2022). PubMed: 34731043
- Ma Y et al. Silencing IQGAP1 alleviates hepatic fibrogenesis via blocking bone marrow mesenchymal stromal cell recruitment to fibrotic liver. Mol Ther Nucleic Acids 27:471-483 (2022). PubMed: 35036058
- Hebert JD et al. The scaffold protein IQGAP1 is crucial for extravasation and metastasis. Sci Rep 10:2439 (2020). PubMed: 32051509
- Sabo Y et al. IQGAP1 Negatively Regulates HIV-1 Gag Trafficking and Virion Production. Cell Rep 30:4065-4081.e4 (2020). PubMed: 32209469
- He C et al. Engineering of HN3 increases the tumor targeting specificity of exosomes and upgrade the anti-tumor effect of sorafenib on HuH-7 cells. PeerJ 8:e9524 (2020). PubMed: 33062407