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RecombinantRabMAb

Recombinant Anti-IQGAP2 antibody [EPR13382-9] (ab187153)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)
  • Western blot - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)
  • Western blot - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)
  • Western blot - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)
  • Anti-IQGAP2 antibody [EPR13382-9] (ab187153)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13382-9] to IQGAP2
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-IQGAP2 antibody [EPR13382-9]
    See all IQGAP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR13382-9] to IQGAP2

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2 and Ramos cell lysate, Rat liver and Mouse testis tissue lysate. IHC-P: Human prostatic hyperplasia, colonic carcinoma and Mouse testis tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab187153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 181 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 181 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human prostatic hyperplasia tissue sections labeling IQGAP2 with Purified ab187153 at 1:100 dilution (1.04 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
  • Western blot - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)
    Western blot - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)
    All lanes : Anti-IQGAP2 antibody [EPR13382-9] (ab187153) at 0.1 µg/ml (purified)

    Lane 1 : Rat liver lysates
    Lane 2 : Mouse testis lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 181 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)
    Western blot - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)
    Anti-IQGAP2 antibody [EPR13382-9] (ab187153) at 0.01 µg/ml (purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 181 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)

    Immunohistochemical analysis of paraffin-embedded Mouse testis labeling IQGAP2 with unpurified ab187153 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)

    Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma labeling IQGAP2 with unpurified  ab187153 at 1/100 dilution.

  • Western blot - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)
    Western blot - Anti-IQGAP2 antibody [EPR13382-9] (ab187153)
    All lanes : Anti-IQGAP2 antibody [EPR13382-9] (ab187153) at 1/1000 dilution (Unpurified)

    Lane 1 : HepG2 cell lysate
    Lane 2 : Ramos cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 181 kDa

  • Anti-IQGAP2 antibody [EPR13382-9] (ab187153)
    Anti-IQGAP2 antibody [EPR13382-9] (ab187153)

References (1)

Publishing research using ab187153? Please let us know so that we can cite the reference in this datasheet.

ab187153 has been referenced in 1 publication.

  • Wang D  et al. LncRNA PDCD4-AS1 alleviates triple negative breast cancer by increasing expression of IQGAP2 via miR-10b-5p. Transl Oncol 14:100958 (2021). PubMed: 33248413

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