Recombinant Anti-IRBIT antibody [EPR13409] (ab178693)
![Western blot - Anti-IRBIT antibody [EPR13409] (ab178693)](https://www.abcam.com/ps/products/178/ab178693/Images/ab178693-206839-anti-irbit-antibody-epr13409-western-blot.jpg "Western blot - Anti-IRBIT antibody [EPR13409] (ab178693)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13409] to IRBIT
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IRBIT antibody [EPR13409]
See all IRBIT primary antibodies -
Description
Rabbit monoclonal [EPR13409] to IRBIT -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human IRBIT aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: O43865 -
Positive control
- Human thymus, HeLa, Ramos and 293T lysates; HeLa cells; 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13409 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178693 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/10000 - 1/50000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
|
|
| ICC/IF |
1/250 - 1/500.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa). |
|
ICC/IF
1/250 - 1/500. |
Target
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Tissue specificity
Expressed in dendritic cells. -
Pathway
Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. -
Sequence similarities
Belongs to the adenosylhomocysteinase family. -
Developmental stage
Expression increases markedly during activation of blood and skin DC (Langerhans cells), but is diminished in terminally differentiated tonsil DC. -
Domain
The PEST region is essential for the interaction with ITPR1. The PDZ-binding region is required for maximal interaction with ITPR1 and is also responsible for the IP3-insensitive interaction with ITPR1. -
Cellular localization
Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 10768 Human
- Entrez Gene: 229709 Mouse
- Entrez Gene: 362013 Rat
- Omim: 607826 Human
- SwissProt: O43865 Human
- SwissProt: Q80SW1 Mouse
- Unigene: 725986 Human
- Unigene: 220328 Mouse
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Alternative names
- AA409031 antibody
- AdoHcyase 2 antibody
- Ahcy-rs3 antibody
see all
Images
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Western blot - Anti-IRBIT antibody [EPR13409] (ab178693)All lanes : Anti-IRBIT antibody [EPR13409] (ab178693) at 1/10000 dilution
Lane 1 : Human thymus lysate
Lane 2 : HeLa cell lysate
Lane 3 : Ramos lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 59 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-IRBIT antibody [EPR13409] (ab178693)](https://www.abcam.com/ps/products/178/ab178693/Images/ab178693-206841-anti-irbit-antibody-epr13409-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IRBIT antibody [EPR13409] (ab178693)Immunofluorescent analysis of HeLa cells labeling IRBIT with ab178693 at 1/250 (red) and DAPI staining (blue).
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![Flow Cytometry (Intracellular) - Anti-IRBIT antibody [EPR13409] (ab178693)](https://www.abcam.com/ps/products/178/ab178693/Images/ab178693-206842-anti-irbit-antibody-epr13409-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-IRBIT antibody [EPR13409] (ab178693)Intracellular flow cytometrical analysis of permeabilized 293T cells labeling IRBIT with ab178693 at 1/10 (red) or a rabbit IgG (negative) (green).
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![Anti-IRBIT antibody [EPR13409] (ab178693)](https://www.abcam.com/ps/products/178/ab178693/Images/ab178693-4-benefits-of-recombinant-antibodies.png)
Anti-IRBIT antibody [EPR13409] (ab178693)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab178693 has been referenced in 3 publications.
- Li X et al. AHCYL1 Is a Novel Biomarker for Predicting Prognosis and Immunotherapy Response in Colorectal Cancer. J Oncol 2022:5054324 (2022). PubMed: 35578598
- García-Berrocoso T et al. Single Cell Immuno-Laser Microdissection Coupled to Label-Free Proteomics to Reveal the Proteotypes of Human Brain Cells After Ischemia. Mol Cell Proteomics 17:175-189 (2018). PubMed: 29133510
- Kruse M et al. Dynamics of Phosphoinositide-Dependent Signaling in Sympathetic Neurons. J Neurosci 36:1386-400 (2016). PubMed: 26818524